Injectable period blood DIY!!

[ENROIDCELL]

Ok, to start this off you need a source that is obviously willing to do this. Personally I already have one which is my gf so if you have one just ask it may take some convincing but just tell her the benefits and everything.

also if you were willing to buy it heres a source u can actually buy menstrual blood if you really can't source it (yes its expensive asf and if you were to buy it, it would be over 5k to get even a small optimal dosage so yeah i don't recommend): Menstrual blood



Here im just gonna list the benefits:

---

1.Systemic IV stem cell infusion: its intended to promote tissue repair throughout the body, reduce inflammation, and actually this potentially aids recovery from injury or just degenerative conditions.

2.Intramuscular (IM) stem cell injections: it is intended to regenerate or strengthen specific muscles or nearby tissues.

3.local clav /epiphyseal injections: stimulating growth plate cartilage or bone remodeling, with the goal of increasing clavicle length.

4.TGF-β, dexamethasone, AA2P, and L-proline mixture: can push stem cells toward cartilage formation (chondrogenesis) before they become bone;

4.topical / oral menstrual stem cells (MenSCs): having regenerative effects through absorption, although not lots of convincing evidence that orally or topically applied stem cells survive, or enter the bloodstream, or can produce systemic benefits but theoretically i believe it can.

5. extremely beneficial when used with synergistic compounds which enhance stem cell survival, differentiation, or tissue regeneration. so theoretically, the topical/ oral menstrual stem cells could work.

Heres the administration protocol that would be the most optimal scientifically, but realizing a lot of this you wouldn't be able to do so you can dnr it!!​

---

Step 1 – Donor Testing​

The donor is screened via laboratory testing and should be negative for:

• HIV-1/2
• HBV (Hepatitis B Virus)
• HCV (Hepatitis C Virus)
• Treponema pallidum (Syphilis)
• HTLV-1/2
• CMV
• WNV antibodies

The image also references collection of approximately 10 mL of menstrual blood;

---

Step 2 – Preparation​

Prepare a pre-sterilized 50 mL aliquot tube containing:

ComponentConcentrationPBSBase solutionAmphotericin B0.25 mg/mLPenicillin/Streptomycin1%EDTA2 mM

---

Step 3 – Sample Collection​

• Donor collects menstrual blood during Days 1–3 of menstruation.
• Sample is transferred into the prepared tube.
• Sample mixture is maintained at 2–8°C.
• Storage period referenced: approximately 48 hours.

---

Step 4 – Isolation​

The image describes:

1. Transfer mixture into a closed processing bag.
2. Centrifuge at 100 × g for 3 minutes.
3. Separate fluid fraction and pellet (solid fraction).
4. Perform red blood cell lysis using RBC lysis buffer.
5. Incubate for approximately 5–10 minutes.
6. Centrifuge at 300 × g for 5 minutes.
7. Discard fluid fraction.
8. Resuspend pellet in 10 mL PBS.
9. Repeat washing and centrifugation steps.

Materials Referenced​

• Closed processing bag
• RBC lysis buffer
• PBS
• Centrifuge

---

Step 5 – Tissue Digestion​

Performed under ISO 5 airflow conditions.

The image references:

• Collagenase I + II
• Concentration: 1–2 mg/mL
• Volume: 5–10 mL
• Closed digestion vessel
• Incubation at 37°C
• Duration: 30–60 minutes

Following digestion:

• Add equal volume of growth medium.
• Filter multiple times through a 40 µm cell strainer.

Materials Referenced​

• ISO 5 airflow workstation
• Closed digestion vessel
• Collagenase I
• Collagenase II
• Growth medium
• 40 µm filter/strainer

---

Step 6 – Combination and Recovery​

• Combine products from Steps 4 and 5.
• Centrifuge at 300 × g for 5 minutes.
• Discard supernatant.
• Add 10 mL growth medium.

---

Step 7 – Cell Counting​

• Remove approximately 10 µL aliquot.
• Count cells.
• Multiply by total sample volume to estimate yield.

Target yield referenced:

• Greater than 1 × 10⁵ cells considered acceptable.

Equipment Referenced​

• Cell counting system
• Hemocytometer or equivalent counter (not specifically stated)

---

Step 8 – Expansion (Primary Culture)​

Transfer sample from Step 6 into a 25 cm² culture vessel.

Culture conditions:

ParameterValueTemperature37°CCO₂5%O₂2–5%

After Day 1:

• Replace spent medium.
• Continue replacing medium every other day through approximately Day 10.

Equipment Referenced​

• Cell culture incubator
• 25 cm² culture flask/plate

---

Step 9 – P0 Harvest​

At approximately 70–80% confluence:

1. Detach cells using trypsin.
2. Centrifuge at 300 × g for 5 minutes.
3. Count cells.

Referenced timing:

• Around Day 10.

---

Step 10 – Expansion Phase 2​

Transfer cells into a 75 cm² culture vessel.

Referenced seeding density:

• 5,000 cells/cm²

Continue culture until:

• Approximately 1 × 10⁶ cells are obtained.

Equipment Referenced​

• 75 cm² culture flask
• Incubator

---

Step 11 – Scale-Up​

Continue repeating expansion cycles until target cell quantities are reached.

The image references various target cell counts for different administration methods, including:

• Clavicular epiphyseal injection
• Intravenous (IV) injection
• Local intramuscular (IM) injection

Specific quantities are stated in the image but are not reproduced here because they are tied directly to proposed administration procedures.

---

Step 12 – Final Harvest​

After desired cell count is reached:

1. Wash cells with PBS.
2. Detach using trypsin at 37°C for 3–5 minutes.
3. Neutralize trypsin.
4. Resuspend cells.
5. Wash and centrifuge at 300 × g for 5 minutes.
6. Repeat wash/resuspension cycle three times.

Final concentration referenced:

• Approximately 1 × 10⁷ cells/mL

Materials Referenced​

• PBS
• Trypsin
• Neutralization medium
• Centrifuge

---

Step 13 – Quality Control​

The image lists testing of a representative aliquot for:

• Sterility
• Endotoxins
• Karyotype
• Additional quality-control assays

---

Equipment and Materials Mentioned in the Image​

Containers and Vessels​

• 50 mL sterile aliquot tube
• Closed processing bag
• Closed digestion vessel
• 25 cm² culture vessel
• 75 cm² culture vessel

Reagents​

• PBS
• Amphotericin B
• Penicillin/Streptomycin
• EDTA
• RBC lysis buffer
• Collagenase I
• Collagenase II
• Growth medium
• Trypsin

Equipment​

• Refrigerated storage (2–8°C)
• Centrifuge
• ISO 5 airflow workstation
• Cell culture incubator
• Cell counter
• 40 µm cell strainer

Quality Control​

• Sterility testing
• Endotoxin testing
• Karyotype analysis

---

Now lets get to the real stuff​

---

1. 1. IV administration -> stem cells travel to desired locations, marked via mechanotransduction:
   - Dose 2.5*10^6 cells/kg bw * x kg of bw - Dose ÷ 10^7 cells/mL => desired mL for infusion - Put into 100 mL bag with 95 mL 0.9% Saline and recount an aliquot before release - Infusion over 30–60 min w monitoring

---

2. IM administration:

- Low dose start at 5*10^5 cells/kg bw, divide dose between injection sides (bilateral deltoid ± bilateral biceps), divide by 10 spots per muscle - Calculate desired mg/mL - Inject 3–5 sec per inj. (this hurts a lot)

---

3. Local clavicular epiphyseal injection:

- Low dose start 5*10^6 cells = 0.5 mL volume (confirm via imaging beforehand) - Titrate up to 4*10^7 cells, keep at the same volume (lower suspension volume used in Step 12) -

Inject ideally with US real time, but at least with anaesthesia - DO NOT inject intraarticular - Inject the 0.5 mL volume over 1 min

High risk approach: Inject 10 ng/mL TGF-β1/3 + 10^-7 Dexamethasone + 50 mcg/mL AA2P + 40 mcg/mL L-Proline per 5×10^5 cells directly or preculture cells in this mix (+α-MEM + ITS-G)

---

4. (jfl)Topical/oral:

- Use original period or MenSCs orally or topically after Dermaroller/stamp or absorb sublingually Synergistic compounds for all administrations: - Breastmilk 500–1000 mL/day split with every meal, sublingually resting 5 min - also the blood can be better when its virgin

Vaginal discharge 0.5 mL/day split a.m./p.m. topical/oral - Female saliva 10+ mL/day topical/oral (optional ofc)

Synergistic compounds for clavicle:

High doses of gh throughout the day.

FGFRi for FGFR1/2 inhibition to prevent osteogenesis at plate rims

KY19382 or I30

- Methylation, pentaosmo and senescence nuke stack

- Compounds ad libitum for EZH1/2, TET, TBX15, ALX4, GLI3, PAX9 and HMGA2



I really spend hours writing that guide, hopefully its helpful!!
NOW GO ASK FOIDS FOR THER MAGICAL LIQUID BOYOOSS

 

[kbj]

dnr fag

 

[GandysOrbitals]

posting straight bs

 

[ghxstsofthepast]

my ratio is nice

 

[itsover369]

Ok, to start this off you need a source that is obviously willing to do this. Personally I already have one which is my gf so if you have one just ask it may take some convincing but just tell her the benefits and everything.

also if you were willing to buy it heres a source u can actually buy menstrual blood if you really can't source it (yes its expensive asf and if you were to buy it, it would be over 5k to get even a small optimal dosage so yeah i don't recommend): Menstrual blood



Here im just gonna list the benefits:

---

1.Systemic IV stem cell infusion: its intended to promote tissue repair throughout the body, reduce inflammation, and actually this potentially aids recovery from injury or just degenerative conditions.

2.Intramuscular (IM) stem cell injections: it is intended to regenerate or strengthen specific muscles or nearby tissues.

3.local clav /epiphyseal injections: stimulating growth plate cartilage or bone remodeling, with the goal of increasing clavicle length.

4.TGF-β, dexamethasone, AA2P, and L-proline mixture: can push stem cells toward cartilage formation (chondrogenesis) before they become bone;

4.topical / oral menstrual stem cells (MenSCs): having regenerative effects through absorption, although not lots of convincing evidence that orally or topically applied stem cells survive, or enter the bloodstream, or can produce systemic benefits but theoretically i believe it can.

5. extremely beneficial when used with synergistic compounds which enhance stem cell survival, differentiation, or tissue regeneration. so theoretically, the topical/ oral menstrual stem cells could work.

Heres the administration protocol that would be the most optimal scientifically, but realizing a lot of this you wouldn't be able to do so you can dnr it!!​

---

Step 1 – Donor Testing​

The donor is screened via laboratory testing and should be negative for:

• HIV-1/2
• HBV (Hepatitis B Virus)
• HCV (Hepatitis C Virus)
• Treponema pallidum (Syphilis)
• HTLV-1/2
• CMV
• WNV antibodies

The image also references collection of approximately 10 mL of menstrual blood;

---

Step 2 – Preparation​

Prepare a pre-sterilized 50 mL aliquot tube containing:

ComponentConcentrationPBSBase solutionAmphotericin B0.25 mg/mLPenicillin/Streptomycin1%EDTA2 mM

---

Step 3 – Sample Collection​

• Donor collects menstrual blood during Days 1–3 of menstruation.
• Sample is transferred into the prepared tube.
• Sample mixture is maintained at 2–8°C.
• Storage period referenced: approximately 48 hours.

---

Step 4 – Isolation​

The image describes:

1. Transfer mixture into a closed processing bag.
2. Centrifuge at 100 × g for 3 minutes.
3. Separate fluid fraction and pellet (solid fraction).
4. Perform red blood cell lysis using RBC lysis buffer.
5. Incubate for approximately 5–10 minutes.
6. Centrifuge at 300 × g for 5 minutes.
7. Discard fluid fraction.
8. Resuspend pellet in 10 mL PBS.
9. Repeat washing and centrifugation steps.

Materials Referenced​

• Closed processing bag
• RBC lysis buffer
• PBS
• Centrifuge

---

Step 5 – Tissue Digestion​

Performed under ISO 5 airflow conditions.

The image references:

• Collagenase I + II
• Concentration: 1–2 mg/mL
• Volume: 5–10 mL
• Closed digestion vessel
• Incubation at 37°C
• Duration: 30–60 minutes

Following digestion:

• Add equal volume of growth medium.
• Filter multiple times through a 40 µm cell strainer.

Materials Referenced​

• ISO 5 airflow workstation
• Closed digestion vessel
• Collagenase I
• Collagenase II
• Growth medium
• 40 µm filter/strainer

---

Step 6 – Combination and Recovery​

• Combine products from Steps 4 and 5.
• Centrifuge at 300 × g for 5 minutes.
• Discard supernatant.
• Add 10 mL growth medium.

---

Step 7 – Cell Counting​

• Remove approximately 10 µL aliquot.
• Count cells.
• Multiply by total sample volume to estimate yield.

Target yield referenced:

• Greater than 1 × 10⁵ cells considered acceptable.

Equipment Referenced​

• Cell counting system
• Hemocytometer or equivalent counter (not specifically stated)

---

Step 8 – Expansion (Primary Culture)​

Transfer sample from Step 6 into a 25 cm² culture vessel.

Culture conditions:

ParameterValueTemperature37°CCO₂5%O₂2–5%

After Day 1:

• Replace spent medium.
• Continue replacing medium every other day through approximately Day 10.

Equipment Referenced​

• Cell culture incubator
• 25 cm² culture flask/plate

---

Step 9 – P0 Harvest​

At approximately 70–80% confluence:

1. Detach cells using trypsin.
2. Centrifuge at 300 × g for 5 minutes.
3. Count cells.

Referenced timing:

• Around Day 10.

---

Step 10 – Expansion Phase 2​

Transfer cells into a 75 cm² culture vessel.

Referenced seeding density:

• 5,000 cells/cm²

Continue culture until:

• Approximately 1 × 10⁶ cells are obtained.

Equipment Referenced​

• 75 cm² culture flask
• Incubator

---

Step 11 – Scale-Up​

Continue repeating expansion cycles until target cell quantities are reached.

The image references various target cell counts for different administration methods, including:

• Clavicular epiphyseal injection
• Intravenous (IV) injection
• Local intramuscular (IM) injection

Specific quantities are stated in the image but are not reproduced here because they are tied directly to proposed administration procedures.

---

Step 12 – Final Harvest​

After desired cell count is reached:

1. Wash cells with PBS.
2. Detach using trypsin at 37°C for 3–5 minutes.
3. Neutralize trypsin.
4. Resuspend cells.
5. Wash and centrifuge at 300 × g for 5 minutes.
6. Repeat wash/resuspension cycle three times.

Final concentration referenced:

• Approximately 1 × 10⁷ cells/mL

Materials Referenced​

• PBS
• Trypsin
• Neutralization medium
• Centrifuge

---

Step 13 – Quality Control​

The image lists testing of a representative aliquot for:

• Sterility
• Endotoxins
• Karyotype
• Additional quality-control assays

---

Equipment and Materials Mentioned in the Image​

Containers and Vessels​

• 50 mL sterile aliquot tube
• Closed processing bag
• Closed digestion vessel
• 25 cm² culture vessel
• 75 cm² culture vessel

Reagents​

• PBS
• Amphotericin B
• Penicillin/Streptomycin
• EDTA
• RBC lysis buffer
• Collagenase I
• Collagenase II
• Growth medium
• Trypsin

Equipment​

• Refrigerated storage (2–8°C)
• Centrifuge
• ISO 5 airflow workstation
• Cell culture incubator
• Cell counter
• 40 µm cell strainer

Quality Control​

• Sterility testing
• Endotoxin testing
• Karyotype analysis

---

Now lets get to the real stuff​

---

1. 1. IV administration -> stem cells travel to desired locations, marked via mechanotransduction:
   - Dose 2.5*10^6 cells/kg bw * x kg of bw - Dose ÷ 10^7 cells/mL => desired mL for infusion - Put into 100 mL bag with 95 mL 0.9% Saline and recount an aliquot before release - Infusion over 30–60 min w monitoring

---

2. IM administration:

- Low dose start at 5*10^5 cells/kg bw, divide dose between injection sides (bilateral deltoid ± bilateral biceps), divide by 10 spots per muscle - Calculate desired mg/mL - Inject 3–5 sec per inj. (this hurts a lot)

---

3. Local clavicular epiphyseal injection:

- Low dose start 5*10^6 cells = 0.5 mL volume (confirm via imaging beforehand) - Titrate up to 4*10^7 cells, keep at the same volume (lower suspension volume used in Step 12) -

Inject ideally with US real time, but at least with anaesthesia - DO NOT inject intraarticular - Inject the 0.5 mL volume over 1 min

High risk approach: Inject 10 ng/mL TGF-β1/3 + 10^-7 Dexamethasone + 50 mcg/mL AA2P + 40 mcg/mL L-Proline per 5×10^5 cells directly or preculture cells in this mix (+α-MEM + ITS-G)

---

4. (jfl)Topical/oral:

- Use original period or MenSCs orally or topically after Dermaroller/stamp or absorb sublingually Synergistic compounds for all administrations: - Breastmilk 500–1000 mL/day split with every meal, sublingually resting 5 min - also the blood can be better when its virgin

Vaginal discharge 0.5 mL/day split a.m./p.m. topical/oral - Female saliva 10+ mL/day topical/oral (optional ofc)

Synergistic compounds for clavicle:

High doses of gh throughout the day.

FGFRi for FGFR1/2 inhibition to prevent osteogenesis at plate rims

KY19382 or I30

- Methylation, pentaosmo and senescence nuke stack

- Compounds ad libitum for EZH1/2, TET, TBX15, ALX4, GLI3, PAX9 and HMGA2



I really spend hours writing that guide, hopefully its helpful!!
NOW GO ASK FOIDS FOR THER MAGICAL LIQUID BOYOOSS

Eww

 

[true leanmaxxer]

 

[iblamejordan1]

Ok, to start this off you need a source that is obviously willing to do this. Personally I already have one which is my gf so if you have one just ask it may take some convincing but just tell her the benefits and everything.

also if you were willing to buy it heres a source u can actually buy menstrual blood if you really can't source it (yes its expensive asf and if you were to buy it, it would be over 5k to get even a small optimal dosage so yeah i don't recommend): Menstrual blood



Here im just gonna list the benefits:

---

1.Systemic IV stem cell infusion: its intended to promote tissue repair throughout the body, reduce inflammation, and actually this potentially aids recovery from injury or just degenerative conditions.

2.Intramuscular (IM) stem cell injections: it is intended to regenerate or strengthen specific muscles or nearby tissues.

3.local clav /epiphyseal injections: stimulating growth plate cartilage or bone remodeling, with the goal of increasing clavicle length.

4.TGF-β, dexamethasone, AA2P, and L-proline mixture: can push stem cells toward cartilage formation (chondrogenesis) before they become bone;

4.topical / oral menstrual stem cells (MenSCs): having regenerative effects through absorption, although not lots of convincing evidence that orally or topically applied stem cells survive, or enter the bloodstream, or can produce systemic benefits but theoretically i believe it can.

5. extremely beneficial when used with synergistic compounds which enhance stem cell survival, differentiation, or tissue regeneration. so theoretically, the topical/ oral menstrual stem cells could work.

Heres the administration protocol that would be the most optimal scientifically, but realizing a lot of this you wouldn't be able to do so you can dnr it!!​

---

Step 1 – Donor Testing​

The donor is screened via laboratory testing and should be negative for:

• HIV-1/2
• HBV (Hepatitis B Virus)
• HCV (Hepatitis C Virus)
• Treponema pallidum (Syphilis)
• HTLV-1/2
• CMV
• WNV antibodies

The image also references collection of approximately 10 mL of menstrual blood;

---

Step 2 – Preparation​

Prepare a pre-sterilized 50 mL aliquot tube containing:

ComponentConcentrationPBSBase solutionAmphotericin B0.25 mg/mLPenicillin/Streptomycin1%EDTA2 mM

---

Step 3 – Sample Collection​

• Donor collects menstrual blood during Days 1–3 of menstruation.
• Sample is transferred into the prepared tube.
• Sample mixture is maintained at 2–8°C.
• Storage period referenced: approximately 48 hours.

---

Step 4 – Isolation​

The image describes:

1. Transfer mixture into a closed processing bag.
2. Centrifuge at 100 × g for 3 minutes.
3. Separate fluid fraction and pellet (solid fraction).
4. Perform red blood cell lysis using RBC lysis buffer.
5. Incubate for approximately 5–10 minutes.
6. Centrifuge at 300 × g for 5 minutes.
7. Discard fluid fraction.
8. Resuspend pellet in 10 mL PBS.
9. Repeat washing and centrifugation steps.

Materials Referenced​

• Closed processing bag
• RBC lysis buffer
• PBS
• Centrifuge

---

Step 5 – Tissue Digestion​

Performed under ISO 5 airflow conditions.

The image references:

• Collagenase I + II
• Concentration: 1–2 mg/mL
• Volume: 5–10 mL
• Closed digestion vessel
• Incubation at 37°C
• Duration: 30–60 minutes

Following digestion:

• Add equal volume of growth medium.
• Filter multiple times through a 40 µm cell strainer.

Materials Referenced​

• ISO 5 airflow workstation
• Closed digestion vessel
• Collagenase I
• Collagenase II
• Growth medium
• 40 µm filter/strainer

---

Step 6 – Combination and Recovery​

• Combine products from Steps 4 and 5.
• Centrifuge at 300 × g for 5 minutes.
• Discard supernatant.
• Add 10 mL growth medium.

---

Step 7 – Cell Counting​

• Remove approximately 10 µL aliquot.
• Count cells.
• Multiply by total sample volume to estimate yield.

Target yield referenced:

• Greater than 1 × 10⁵ cells considered acceptable.

Equipment Referenced​

• Cell counting system
• Hemocytometer or equivalent counter (not specifically stated)

---

Step 8 – Expansion (Primary Culture)​

Transfer sample from Step 6 into a 25 cm² culture vessel.

Culture conditions:

ParameterValueTemperature37°CCO₂5%O₂2–5%

After Day 1:

• Replace spent medium.
• Continue replacing medium every other day through approximately Day 10.

Equipment Referenced​

• Cell culture incubator
• 25 cm² culture flask/plate

---

Step 9 – P0 Harvest​

At approximately 70–80% confluence:

1. Detach cells using trypsin.
2. Centrifuge at 300 × g for 5 minutes.
3. Count cells.

Referenced timing:

• Around Day 10.

---

Step 10 – Expansion Phase 2​

Transfer cells into a 75 cm² culture vessel.

Referenced seeding density:

• 5,000 cells/cm²

Continue culture until:

• Approximately 1 × 10⁶ cells are obtained.

Equipment Referenced​

• 75 cm² culture flask
• Incubator

---

Step 11 – Scale-Up​

Continue repeating expansion cycles until target cell quantities are reached.

The image references various target cell counts for different administration methods, including:

• Clavicular epiphyseal injection
• Intravenous (IV) injection
• Local intramuscular (IM) injection

Specific quantities are stated in the image but are not reproduced here because they are tied directly to proposed administration procedures.

---

Step 12 – Final Harvest​

After desired cell count is reached:

1. Wash cells with PBS.
2. Detach using trypsin at 37°C for 3–5 minutes.
3. Neutralize trypsin.
4. Resuspend cells.
5. Wash and centrifuge at 300 × g for 5 minutes.
6. Repeat wash/resuspension cycle three times.

Final concentration referenced:

• Approximately 1 × 10⁷ cells/mL

Materials Referenced​

• PBS
• Trypsin
• Neutralization medium
• Centrifuge

---

Step 13 – Quality Control​

The image lists testing of a representative aliquot for:

• Sterility
• Endotoxins
• Karyotype
• Additional quality-control assays

---

Equipment and Materials Mentioned in the Image​

Containers and Vessels​

• 50 mL sterile aliquot tube
• Closed processing bag
• Closed digestion vessel
• 25 cm² culture vessel
• 75 cm² culture vessel

Reagents​

• PBS
• Amphotericin B
• Penicillin/Streptomycin
• EDTA
• RBC lysis buffer
• Collagenase I
• Collagenase II
• Growth medium
• Trypsin

Equipment​

• Refrigerated storage (2–8°C)
• Centrifuge
• ISO 5 airflow workstation
• Cell culture incubator
• Cell counter
• 40 µm cell strainer

Quality Control​

• Sterility testing
• Endotoxin testing
• Karyotype analysis

---

Now lets get to the real stuff​

---

1. 1. IV administration -> stem cells travel to desired locations, marked via mechanotransduction:
   - Dose 2.5*10^6 cells/kg bw * x kg of bw - Dose ÷ 10^7 cells/mL => desired mL for infusion - Put into 100 mL bag with 95 mL 0.9% Saline and recount an aliquot before release - Infusion over 30–60 min w monitoring

---

2. IM administration:

- Low dose start at 5*10^5 cells/kg bw, divide dose between injection sides (bilateral deltoid ± bilateral biceps), divide by 10 spots per muscle - Calculate desired mg/mL - Inject 3–5 sec per inj. (this hurts a lot)

---

3. Local clavicular epiphyseal injection:

- Low dose start 5*10^6 cells = 0.5 mL volume (confirm via imaging beforehand) - Titrate up to 4*10^7 cells, keep at the same volume (lower suspension volume used in Step 12) -

Inject ideally with US real time, but at least with anaesthesia - DO NOT inject intraarticular - Inject the 0.5 mL volume over 1 min

High risk approach: Inject 10 ng/mL TGF-β1/3 + 10^-7 Dexamethasone + 50 mcg/mL AA2P + 40 mcg/mL L-Proline per 5×10^5 cells directly or preculture cells in this mix (+α-MEM + ITS-G)

---

4. (jfl)Topical/oral:

- Use original period or MenSCs orally or topically after Dermaroller/stamp or absorb sublingually Synergistic compounds for all administrations: - Breastmilk 500–1000 mL/day split with every meal, sublingually resting 5 min - also the blood can be better when its virgin

Vaginal discharge 0.5 mL/day split a.m./p.m. topical/oral - Female saliva 10+ mL/day topical/oral (optional ofc)

Synergistic compounds for clavicle:

High doses of gh throughout the day.

FGFRi for FGFR1/2 inhibition to prevent osteogenesis at plate rims

KY19382 or I30

- Methylation, pentaosmo and senescence nuke stack

- Compounds ad libitum for EZH1/2, TET, TBX15, ALX4, GLI3, PAX9 and HMGA2



I really spend hours writing that guide, hopefully its helpful!!
NOW GO ASK FOIDS FOR THER MAGICAL LIQUID BOYOOSS

All started with mewing btw

 

[User7]

We made too many humans...

 

[Organ]

Ok, to start this off you need a source that is obviously willing to do this. Personally I already have one which is my gf so if you have one just ask it may take some convincing but just tell her the benefits and everything.

also if you were willing to buy it heres a source u can actually buy menstrual blood if you really can't source it (yes its expensive asf and if you were to buy it, it would be over 5k to get even a small optimal dosage so yeah i don't recommend): Menstrual blood



Here im just gonna list the benefits:

---

1.Systemic IV stem cell infusion: its intended to promote tissue repair throughout the body, reduce inflammation, and actually this potentially aids recovery from injury or just degenerative conditions.

2.Intramuscular (IM) stem cell injections: it is intended to regenerate or strengthen specific muscles or nearby tissues.

3.local clav /epiphyseal injections: stimulating growth plate cartilage or bone remodeling, with the goal of increasing clavicle length.

4.TGF-β, dexamethasone, AA2P, and L-proline mixture: can push stem cells toward cartilage formation (chondrogenesis) before they become bone;

4.topical / oral menstrual stem cells (MenSCs): having regenerative effects through absorption, although not lots of convincing evidence that orally or topically applied stem cells survive, or enter the bloodstream, or can produce systemic benefits but theoretically i believe it can.

5. extremely beneficial when used with synergistic compounds which enhance stem cell survival, differentiation, or tissue regeneration. so theoretically, the topical/ oral menstrual stem cells could work.

Heres the administration protocol that would be the most optimal scientifically, but realizing a lot of this you wouldn't be able to do so you can dnr it!!​

---

Step 1 – Donor Testing​

The donor is screened via laboratory testing and should be negative for:

• HIV-1/2
• HBV (Hepatitis B Virus)
• HCV (Hepatitis C Virus)
• Treponema pallidum (Syphilis)
• HTLV-1/2
• CMV
• WNV antibodies

The image also references collection of approximately 10 mL of menstrual blood;

---

Step 2 – Preparation​

Prepare a pre-sterilized 50 mL aliquot tube containing:

ComponentConcentrationPBSBase solutionAmphotericin B0.25 mg/mLPenicillin/Streptomycin1%EDTA2 mM

---

Step 3 – Sample Collection​

• Donor collects menstrual blood during Days 1–3 of menstruation.
• Sample is transferred into the prepared tube.
• Sample mixture is maintained at 2–8°C.
• Storage period referenced: approximately 48 hours.

---

Step 4 – Isolation​

The image describes:

1. Transfer mixture into a closed processing bag.
2. Centrifuge at 100 × g for 3 minutes.
3. Separate fluid fraction and pellet (solid fraction).
4. Perform red blood cell lysis using RBC lysis buffer.
5. Incubate for approximately 5–10 minutes.
6. Centrifuge at 300 × g for 5 minutes.
7. Discard fluid fraction.
8. Resuspend pellet in 10 mL PBS.
9. Repeat washing and centrifugation steps.

Materials Referenced​

• Closed processing bag
• RBC lysis buffer
• PBS
• Centrifuge

---

Step 5 – Tissue Digestion​

Performed under ISO 5 airflow conditions.

The image references:

• Collagenase I + II
• Concentration: 1–2 mg/mL
• Volume: 5–10 mL
• Closed digestion vessel
• Incubation at 37°C
• Duration: 30–60 minutes

Following digestion:

• Add equal volume of growth medium.
• Filter multiple times through a 40 µm cell strainer.

Materials Referenced​

• ISO 5 airflow workstation
• Closed digestion vessel
• Collagenase I
• Collagenase II
• Growth medium
• 40 µm filter/strainer

---

Step 6 – Combination and Recovery​

• Combine products from Steps 4 and 5.
• Centrifuge at 300 × g for 5 minutes.
• Discard supernatant.
• Add 10 mL growth medium.

---

Step 7 – Cell Counting​

• Remove approximately 10 µL aliquot.
• Count cells.
• Multiply by total sample volume to estimate yield.

Target yield referenced:

• Greater than 1 × 10⁵ cells considered acceptable.

Equipment Referenced​

• Cell counting system
• Hemocytometer or equivalent counter (not specifically stated)

---

Step 8 – Expansion (Primary Culture)​

Transfer sample from Step 6 into a 25 cm² culture vessel.

Culture conditions:

ParameterValueTemperature37°CCO₂5%O₂2–5%

After Day 1:

• Replace spent medium.
• Continue replacing medium every other day through approximately Day 10.

Equipment Referenced​

• Cell culture incubator
• 25 cm² culture flask/plate

---

Step 9 – P0 Harvest​

At approximately 70–80% confluence:

1. Detach cells using trypsin.
2. Centrifuge at 300 × g for 5 minutes.
3. Count cells.

Referenced timing:

• Around Day 10.

---

Step 10 – Expansion Phase 2​

Transfer cells into a 75 cm² culture vessel.

Referenced seeding density:

• 5,000 cells/cm²

Continue culture until:

• Approximately 1 × 10⁶ cells are obtained.

Equipment Referenced​

• 75 cm² culture flask
• Incubator

---

Step 11 – Scale-Up​

Continue repeating expansion cycles until target cell quantities are reached.

The image references various target cell counts for different administration methods, including:

• Clavicular epiphyseal injection
• Intravenous (IV) injection
• Local intramuscular (IM) injection

Specific quantities are stated in the image but are not reproduced here because they are tied directly to proposed administration procedures.

---

Step 12 – Final Harvest​

After desired cell count is reached:

1. Wash cells with PBS.
2. Detach using trypsin at 37°C for 3–5 minutes.
3. Neutralize trypsin.
4. Resuspend cells.
5. Wash and centrifuge at 300 × g for 5 minutes.
6. Repeat wash/resuspension cycle three times.

Final concentration referenced:

• Approximately 1 × 10⁷ cells/mL

Materials Referenced​

• PBS
• Trypsin
• Neutralization medium
• Centrifuge

---

Step 13 – Quality Control​

The image lists testing of a representative aliquot for:

• Sterility
• Endotoxins
• Karyotype
• Additional quality-control assays

---

Equipment and Materials Mentioned in the Image​

Containers and Vessels​

• 50 mL sterile aliquot tube
• Closed processing bag
• Closed digestion vessel
• 25 cm² culture vessel
• 75 cm² culture vessel

Reagents​

• PBS
• Amphotericin B
• Penicillin/Streptomycin
• EDTA
• RBC lysis buffer
• Collagenase I
• Collagenase II
• Growth medium
• Trypsin

Equipment​

• Refrigerated storage (2–8°C)
• Centrifuge
• ISO 5 airflow workstation
• Cell culture incubator
• Cell counter
• 40 µm cell strainer

Quality Control​

• Sterility testing
• Endotoxin testing
• Karyotype analysis

---

Now lets get to the real stuff​

---

1. 1. IV administration -> stem cells travel to desired locations, marked via mechanotransduction:
   - Dose 2.5*10^6 cells/kg bw * x kg of bw - Dose ÷ 10^7 cells/mL => desired mL for infusion - Put into 100 mL bag with 95 mL 0.9% Saline and recount an aliquot before release - Infusion over 30–60 min w monitoring

---

2. IM administration:

- Low dose start at 5*10^5 cells/kg bw, divide dose between injection sides (bilateral deltoid ± bilateral biceps), divide by 10 spots per muscle - Calculate desired mg/mL - Inject 3–5 sec per inj. (this hurts a lot)

---

3. Local clavicular epiphyseal injection:

- Low dose start 5*10^6 cells = 0.5 mL volume (confirm via imaging beforehand) - Titrate up to 4*10^7 cells, keep at the same volume (lower suspension volume used in Step 12) -

Inject ideally with US real time, but at least with anaesthesia - DO NOT inject intraarticular - Inject the 0.5 mL volume over 1 min

High risk approach: Inject 10 ng/mL TGF-β1/3 + 10^-7 Dexamethasone + 50 mcg/mL AA2P + 40 mcg/mL L-Proline per 5×10^5 cells directly or preculture cells in this mix (+α-MEM + ITS-G)

---

4. (jfl)Topical/oral:

- Use original period or MenSCs orally or topically after Dermaroller/stamp or absorb sublingually Synergistic compounds for all administrations: - Breastmilk 500–1000 mL/day split with every meal, sublingually resting 5 min - also the blood can be better when its virgin

Vaginal discharge 0.5 mL/day split a.m./p.m. topical/oral - Female saliva 10+ mL/day topical/oral (optional ofc)

Synergistic compounds for clavicle:

High doses of gh throughout the day.

FGFRi for FGFR1/2 inhibition to prevent osteogenesis at plate rims

KY19382 or I30

- Methylation, pentaosmo and senescence nuke stack

- Compounds ad libitum for EZH1/2, TET, TBX15, ALX4, GLI3, PAX9 and HMGA2



I really spend hours writing that guide, hopefully its helpful!!
NOW GO ASK FOIDS FOR THER MAGICAL LIQUID BOYOOSS

Contributing negative information to this forum

 

[larpsahurcel]

Rich get richer huh

 

[ENROIDCELL]

Contributing negative information to this forum

WHAT IS NEGATIV ABT FUCKASS NGGA

 

[ENROIDCELL]

View attachment 5493899