melatonin dosage

[orgㅤ]

whats the max amount i can take that wont have any crazy sides.

some say 5mg some say 10mg some say fucking 50mg and some say 1mg im confused

 

[orgㅤ]

b

 

[Aiwass]

1 mg is all u need for sleep, anything more jut has antioxidant effects

too much antioxidants are pro-oxidant

only take high amounts after drinking

 

[orgㅤ]

1 mg is all u need for sleep, anything more jut has antioxidant effects

too much antioxidants are pro-oxidant

only take high amounts after drinking

1mg doesn't do shit for me tho man

 

[Aiwass]

@NoaA99 why jfl? i am correct

1 mg is enough to saturate the mt1/2 receptors

 

[Aiwass]

1mg doesn't do shit for me tho man

because melatonin just helps ur circadian rhythm, its not a sedative

 

[orgㅤ]

because melatonin just helps ur circadian rhythm, its not a sedative

so 1mg and 5 mg are the same?

 

[Aiwass]

so 1mg and 5 mg are the same?

for sleep, yeah (for most people depending on receptor sensitivity)

 

[NoaA99]

@NoaA99 why jfl? i am correct

1 mg is enough to saturate the mt1/2 receptors

Do you know how a free antioxidant works? Receptor saturation = no further effects -> False.
There is no evidence that melatonin becomes pro-oxidant, unlike certain other antioxidants.

„Melatonin is considered a potent natural antioxidant, not only due to the direct inhibition of ROS, but also due to the mobilization of the intracellular antioxidative enzyme system.

Melatonin can protect MSCs against oxidation-induced apoptosis by reducing ROS production, enhancing cell viability and promoting continued differentiation.“

„Melatonin can increase solute carrier family 39 member 1 expression, activate the mitogen-activated protein kinase (MAPK)/ERK pathway, increase phosphorylated-ERK1/2/5 levels and significantly inhibit the production of ROS; moreover, zinc uptake in cells is increased“

„Melatonin treatment has been found to elevate the level of VEGF during granulation tissue formation and accelerate the angiogenic process, indicating that melatonin can provide beneficial effects to bone defect repair with vascular injury.“

„Melatonin can reduce the phosphorylation of p65 and IκBα, thereby inhibiting downstream NF-κB signaling pathway activation, which plays a key role in metabolism, inflammation and apoptosis

Take it if you tolerate it.

 

[NoaA99]

for sleep, yeah (for most people depending on receptor sensitivity)

For sleep yes.

 

[Ahmed88]

200mg for 8ius of hgh

 

[Aiwass]

Do you know how a free antioxidant works? Receptor saturation = no further effects -> False.
There is no evidence that melatonin becomes pro-oxidant, unlike certain other antioxidants.

„Melatonin is considered a potent natural antioxidant, not only due to the direct inhibition of ROS, but also due to the mobilization of the intracellular antioxidative enzyme system.

Melatonin can protect MSCs against oxidation-induced apoptosis by reducing ROS production, enhancing cell viability and promoting continued differentiation.“

„Melatonin can increase solute carrier family 39 member 1 expression, activate the mitogen-activated protein kinase (MAPK)/ERK pathway, increase phosphorylated-ERK1/2/5 levels and significantly inhibit the production of ROS; moreover, zinc uptake in cells is increased“

„Melatonin treatment has been found to elevate the level of VEGF during granulation tissue formation and accelerate the angiogenic process, indicating that melatonin can provide beneficial effects to bone defect repair with vascular injury.“

„Melatonin can reduce the phosphorylation of p65 and IκBα, thereby inhibiting downstream NF-κB signaling pathway activation, which plays a key role in metabolism, inflammation and apoptosis

Take it if you tolerate it.

all antioxidants can become pro-oxidant at high doses, retard

Evidence of prooxidant and antioxidant action of melatonin on human liver cell line HepG2

The aim of this study was to evaluate melatonin cytotoxicity by measuring its effects on various cellular targets. Cell viability, intracellular reduc…

www.sciencedirect.com

after mt1/2 receptors are saturated melatonin works on the mt3 receptor which is responsible for the antioxidant effects

also just lol at copy and pasting a bunch of chatgpt bullshit that is completely irrelevant to my point

For sleep yes.

for sleep, yeah

 

[NoaA99]

all antioxidants can become pro-oxidant at high doses, retard

Evidence of prooxidant and antioxidant action of melatonin on human liver cell line HepG2

The aim of this study was to evaluate melatonin cytotoxicity by measuring its effects on various cellular targets. Cell viability, intracellular reduc…

www.sciencedirect.com

after mt1/2 receptors are saturated melatonin works on the mt3 receptor which is responsible for the antioxidant effects

also just lol at copy and pasting a bunch of chatgpt bullshit that is completely irrelevant to my point

Those are quotes from studies. Why would GPT word it like that? It says more about you, whether you noticed or not.

Lmao, those are from in vitro studies on cancer cells, not actual human data.

Melatonin has a Methoxy group that basically acts like a safety cap. This structure prevents MLT from becoming pro-oxidant
After neutralizing a radical, the antioxidant becomes a radical. Most antioxidants get recycled, and during that phase, they can become unstable and pro-oxidative.
MLT never even lives long enough to become what you make it out to be, unlike in your artificial vitro study, which literally has almost zero relevance for practical purposes in humans.

https://www.researchgate.net/publication/12418973_Significance_of_Melatonin_in_Antioxidative_Defense_System_Reactions_and_Products

In the HepG2 study, they put a massive continuous amount of Melatonin into a plastic dish with isolated cancer cells for 96 hours!!! They literally wanted to force a reaction.
There is no liver to process/ metabolize the Melatonin, no kidneys to filter it, no blood flow to distribute it asf. It is only relevant within that specific framework.
Melatonin is supposed to be a pro-oxidant in cancer cells, In normal cells, it is overwhelmingly an antioxidant.

Even at high supplemental doses, melatonin consistently reduces oxidative stress and subsequently inflammation in humans, and you are, for whatever reason, attempting to scare people away from using 10mg+/ daily.

---

 

[Aiwass]

Those are quotes from studies. Why would GPT word it like that? It says more about you, whether you noticed or not.

doesnt negate the fact that its all completely irrelevant

Lmao, those are from in vitro studies on cancer cells, not actual human data.

Melatonin has a Methoxy group that basically acts like a safety cap. This structure prevents MLT from becoming pro-oxidant
After neutralizing a radical, the antioxidant becomes a radical. Most antioxidants get recycled, and during that phase, they can become unstable and pro-oxidative.

thats an oversimplification

melatonin can still turn pro-oxidant, the methoxy group just makes it slightly harder

MLT never even lives long enough to become what you make it out to be, unlike in your artificial vitro study, which literally has almost zero relevance for practical purposes in humans.

https://www.researchgate.net/publication/12418973_Significance_of_Melatonin_in_Antioxidative_Defense_System_Reactions_and_Products

at least quote the study, im not reading it all

In the HepG2 study, they put a massive continuous amount of Melatonin into a plastic dish with isolated cancer cells for 96 hours!!! They literally wanted to force a reaction.
There is no liver to process/ metabolize the Melatonin, no kidneys to filter it, no blood flow to distribute it asf. It is only relevant within that specific framework.
Melatonin is supposed to be a pro-oxidant in cancer cells, In normal cells, it is overwhelmingly an antioxidant.

Even at high supplemental doses, melatonin consistently reduces oxidative stress and subsequently inflammation in humans, and you are, for whatever reason, attempting to scare people away from using 10mg+/ daily.

---

im just cautioning people that more =/= better and that taking very high doses such as 100 mg for antioxidant properties can be counterproductive

 

[rraymond]

whats the max amount i can take that wont have any crazy sides.

some say 5mg some say 10mg some say fucking 50mg and some say 1mg im confused

probably between 5 and 10. who the fuck said 50 and 1
10 is a lot and can like fuck up your dreams or something (im considering doing this just to see if i dream what will it be like, my prescription is 2mg but i normally take 3+)
apparently youll also feel like shit the next day.

so probably about 5mg or a bit over.

 

[NoaA99]

melatonin can still turn pro-oxidant, the methoxy group just makes it slightly harder

"An important contribution of the methoxy group is to prohibit pro-oxidative reactions of the indoles.
The protective methoxy group at 5 position completely prevents quinone-imine formation and radical and redoxreaction-promoting activities of the hydroquinone-semiquinone-quinone-imine type.
This type of reactivity is common for the pro- and antioxidative activities of antioxidants containing hydroxyl groups"
-> That pathway is the main reason antioxidants flip pro-oxidant btw, so why "slightly"?
Melatonin lacks a free-HO group, which is a major reason it does not undergo the usual pro-oxidant cycling.
You suggest a false equivalence (to other hydroxyll-based antioxidants), that is not there.

im just cautioning people that more =/= better and that taking very high doses such as 100 mg for antioxidant properties can be counterproductive

"The antioxidant capacity of melatonin is in fact a combination of the levels of melatonin and its related metabolites. In the studies cited above, the free radical generating agents were also administered in pharmacological concentrations.
It is not practical to expect that physiological levels of any antioxidant can protect the damage induced by pharmacological levels of oxidants"
-> Obvious, no physiological dosage of an antioxidant can necessarily neutralize suprapyhsiological levels of ROS, which would be important for PEDs enhanced people for instance.

Can you send me a vivo study, that proves, that it does?

"We found that melatonin attenuates glutamate-induced cell death of cultured motoneurons. In SOD1(G93A)-transgenic mice, high-dose oral melatonin delayed disease progression and extended survival.
In a clinical safety study, chronic high-dose (300 mg/day) rectal melatonin was well tolerated during an observation period of up to 2 yr.
Importantly, circulating serum protein carbonyls, which provide a surrogate marker for oxidative stress, were elevated in ALS patients, but were normalized to control values by melatonin treatment. This combination of preclinical effectiveness and proven safety in humans suggests that high-dose melatonin is suitable for clinical trials aimed at neuroprotection through antioxidation in ALS"

thats an oversimplification

Simplification is unavoidable for most in biochemistry. Simplifying without distorting the underlying biology is obviously important, though.

at least quote the study, im not reading it all

You literally sent me an abstract with the option to buy the study mate. What did you expect?