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"Stretching the intestine by just walking briskly or even a 10sec jog will produce serotonin. Bacteria are the main factor at rest but mechanical stimulation will also make gut cells produce serotonin. Peristalsis is not driven only by serotonin. Serotonin's role is simply to signal a toxic event and thus "urge" bowel emptying. You can digest fine with even 1% of the baseline serotonin in people with gut flora."