I might not be an expert in anti-aging, but I know a fair amount and have 33 solutions. Plus, I’m good with Photoshop and can build Discord servers, so I can definitely contribute.
He needs to DIY more stuff. He can't be slaying what's wrong with him? It's more important to impress Dalits on .org.
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Not really, I guess just diet and exercise can help a bit.
Its about t4 İ think
Nigga copied my profile picture in his signature
yeah ngl. i got no idea what base pair means. i just got the millions of dollars thing from ur old thread about shapeshifting into sean opry if u get his sweat or something like that.
So a genetic code that is ATGUAGCAUACGA costs like $2.40 maximum to manufacture.
so is this the theory in this thread realistic or no?
I agree
it looked so cool i needed it in my signNigga copied my profile picture in his signature
intredasting
I'm taking nutrition and physiological chem rn
Only 4k? I'm sure thus is more expensive than that. Are you including costs such as pcr machine etc.
why do you say this is crazy?
not doin allatHeightmaxxing Breakthrough: Activating Growth Plate Stem Cells to Grow Taller!
Alright guys, so @mathis was going to release a heightmaxxing guide, but he mentioned it was experimental and expensive. I did some digging to figure out what he could be talking about and quickly realized he was likely referring to activating growth plate stem cells to grow taller. They’ve only done it on rats so far, but the results have been really good. Since his thread is going to take a couple of weeks, I figured I’d get my thread out first:
I will drop THE BIGGEST HEIGHTMAXXING SECRET this week ( NUCLEAR THREAD )
I just discovered the biggest shit ever. You can grow ( actual TARGETED bones growth ) after puberty. Not 1 or 2 cm. 10cm easy. Just remember Its purely theory even thought it probably work you’ll need to be very careful. Some of this shit may cost a lot or may be difficult to find thought. I...looksmax.org
The Study:
View attachment 3134381
TLDR:
- Hedgehog Signaling Pathway: Activating this pathway can stimulate bone growth, but it's most effective when growth plates are still open.
- Heightmaxxing with closed growth plates: This won't work for adults with closed growth plates, as they no longer have the cartilage necessary for bone growth. While it’s a promising avenue for younger individuals with active growth plates, it won’t lead to significant height increases in adults.
In short, it will increase your height by a shit ton and it might even be possible to DIY HYPOTHETICALLY!!!
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DIY METHOD (I doubt anyone will do this it's too expensive and too complicated for most people but I just wanted to put it out there that it is possible.)
Here is everything you need to know about doing it at home. (I DO NOT CONDONE DOING THIS AT HOME. THIS IS HYPOTHETICAL.)
What is CRISPR-Cas9? (Super Important)
CRISPR-Cas9 is an incredible tool that allows scientists to make super precise edits to DNA in living cells.
- CRISPR stands for Clustered Regularly Interspaced Short Palindromic Repeats. It’s a natural defense system used by bacteria to protect themselves from viruses.
- Cas9 is an enzyme that acts like molecular scissors, cutting DNA at specific locations.
CRISPR-Cas9 Components and Cas9 Nuclease
Function:
Amount Needed:
Tools and Equipment:
Best Option:
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Plasmids
Plasmids are like the delivery dudes for gRNA and Cas9 into cells. You need to use about 1-10 µg of plasmid DNA per experiment. To make sure you’ve got the right stuff, you’ll have to use a Gel Electrophoresis Setup, which is basically a way to check if your DNA is good to go. You measure the plasmid DNA and then run it through the gel to verify it. If you’re trying not to blow all your cash, you could even build your own gel setup or hunt for cheap options online.
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Delivery Vectors
Viral vectors, like Adenovirus or Lentivirus, are used to deliver CRISPR components into cells. You’ll need 10^6 to 10^9 viral particles per mL to make it work. To purify these viral vectors, a centrifuge is required. The process involves diluting the vectors and then using the centrifuge to clean them up. If you’re trying to save some money, mini-centrifuges or second-hand equipment can be solid options.
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Study: https://pubmed.ncbi.nlm.nih.gov/26469046/
Growing Cells
To grow cells, you’ll need about 500 mL to 1 L of culture media, like DMEM or RPMI 1640, for each culture. Keeping things sterile is important, so using a Laminar Flow Hood is recommended. But if you don’t have one, setting up a clean workspace in a well-ventilated room can work too. The process is pretty simple: just pour the media into a flask, then add some Fetal Bovine Serum (FBS) and antibiotics.
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- FBS: Add 5-20% of the total media volume.
- Antibiotics (Penicillin/Streptomycin): Add 1% of the media volume to keep bacteria from messing things up.
It’s taking me too long to find a source for everything. I’m sure you can find a source somewhere for these ingredients.
Growth Factors and Chemicals
SAG (Smoothened Agonist): This stuff kicks off pathways that help cells grow. You’ll want to use a concentration of 1-10 µM. If you want to see how it’s working, you can use a Microplate Reader, but if you’re on a budget, a regular spectrophotometer works too. Just dilute the SAG and add it to your cell cultures.
IGF-1 (Recombinant Human IGF-1): This promotes cell growth and keeps them alive. You’ll need to add 10-100 ng/mL to your culture media.
Tamoxifen: This controls gene expression by hooking up with estrogen receptors. Use a concentration of 1-10 µM for this one.
Tamoxifen, 98%, Thermo Scientific Chemicals
Tamoxifen, 10540-29-1, is an antineoplastic agent that acts by competitively binding estrogen receptors to reduce DNA synthesis. Learn more at Thermo Fisher Scientific.www.thermofisher.com
Verification and Analysis
PCR (Polymerase Chain Reaction): PCR is a technique used to make a bunch of copies of a specific DNA segment so you can analyze it. To do this, you'll need a concentration of about 0.1-1 µM and a machine called a Thermal Cycler
Sequencing (Sanger or Next-Generation): Sequencing is the method used to figure out the exact order of DNA bases, which helps you confirm any changes or edits you made. The amount of DNA you’ll need depends on which sequencing service you’re using.
View attachment 3134620
1. Getting the Solution Ready
First off, you need to mix up the solution:
- Sterilization: Make sure all your gear (like syringes, needles, and vials) is clean and sterilized to prevent contamination.
- Mixing It Up: Combine the SAG, IGF-1, and Tamoxifen in a clean vial. Give it a gentle swirl to mix everything—don’t shake it too hard, or you might mess up the mixture!
2. Filling the Syringe
Next, you’ve gotta get the solution into the syringe:
- Draw It Up: Attach the needle to the syringe and carefully draw the solution into it. Be sure to avoid any air bubbles.
- Check the Amount: Double-check that you’ve got the right amount in the syringe. Accurate dosing is crucial.
- Example Dosages: If you’re injecting 100 µL per site, ensure your mix has the correct amount of each ingredient.
3. Prepping the Injection Spot
Now, let’s get the injection site ready:
View attachment 3134721
- Keep It Clean: Perform this step in a very clean environment to avoid any contamination.
- Find the Secondary Ossification Center: While it’s best to use imaging tools like X-rays, if that’s not an option, you can locate the general area based on anatomical landmarks. The secondary ossification center is typically located near the ends of long bones, such as the femur, close to the joint. You might feel for the softer areas at the ends of bones where growth plates are located.
- Mark It: Once you’ve identified the area near the joint where the secondary ossification center is likely located, mark the spot carefully to ensure accurate injection.
4. Doing the Injection
Here’s how you do the injection:
- Clean the Area: Wipe down the marked spot with antiseptic to keep it clean.
- Stick the Needle In: Carefully insert the needle into the marked spot, aiming for the softer, slightly indented area near the joint, which is indicative of the secondary ossification center.
- Inject the Solution: Slowly inject the solution into the area. Do it steadily to ensure it goes in smoothly.
- How Much to Use: Typically, 100 µL per secondary ossification center should be sufficient, as suggested by the study. (Since you're a human and not a rat, the dosages might not be sufficient. This is all hypothetical, though, lol)
Well, this was fun to make. I doubt anyone will actually do this it's all hypothetical and costs a shit ton of money. Plus, it hasn't been tested in human trials yet, so the dosages might be off depending on your weight and other factors. I'm just going by the study, but yeah. I probably messed up a few times because I'm not a scientist, but let me know your thoughts do you think this would work on humans?
Gonna raise a mogger kid who'll never understand what a blessing I brought to him
It's a good one, but our hopes are on the @mathis topic and not on this one
Ur not going to do it
If this in a Uk uni I am willing to fund and source this potentially
serms agonise the estrogen receptors in the growth plates no?
Tell me if yall make it happen or if i can help. Sound wonderfull.
You guys can go ahead. I have other things to do.
