enchanted_elixir
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The Most Powerful & Versatile Looksmaxing Technique Revealed
by @enchanted_elixir
Table Of Contentsby @enchanted_elixir
- Introduction
- What are induced pluripotent stem cells?
- How can this be done?
- The power of combining pluripotent stem cells with genetic/epigenetic editing
- Conclusion
As an apology to the members of the forum for "blue-balling" everyone
. I believe that you deserve this knowledge. So here we go. I will try my best to dumb things down for you all. Note: Unless you have a decent amount of money and smart enough to replicate what Shinya Yamanaka did, this won't be applicable as of now. Also, this will be oversimplified so DYOR.What are induced pluripotent stem cells?
To dumb it down for you, let's get into the basics. I assume everyone knows what a cell is already so... What is a stem cell? Well a stem cell is a cell with the ability to turn into any kind of cell into the body, whether it is a nerve cell, an iris cell, a muscle cell, a "growth plate" cell, etc.
With stem cells, there are certain tiers to these cells' potency.
- Totipotent - Can turn into any cell, including an embryo
- Pluripotent - Can turn into any cell, excluding an embryo
- Multipotent - Can turn into a lot of cells, but not every cell
- Oligopotent - Can turn into a few types of cells
- Unipotent - Can turn into one kind of cell
Shinya Yamanaka discovered four genes that the biotech space now calls "Yamanaka" factors. The Yamanaka factors (Oct3/4, Sox2, Klf4, c-Myc) are a group of protein transcription factors (which means these genes control how other genes get turned on or off) that play a vital role in the creation of induced pluripotent stem cells (cells that have the ability to become any cell in the body), often called iPSCs. They control how DNA is copied for translation into other proteins.
This is literally one of the best discoveries of all time! He won the Nobel Prize of Medicine in 2012 for this. This is REALLY POWERFUL!
Papers on this topic:
- https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6682501/
- https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3584308/
- https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4313779/
- https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3278994/
- https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5118044/
How can this be done?
So basically, I plan on following studies that I can find published on induced pluripotent stem cells on https://www.ncbi.nlm.nih.gov. But here is the basic guidelines on how to do it (oversimplified, because if it is not, you won't get it)
- Extract your blood or skin cells (deep in your skin I believe, not the top layer) and insert them in a petri dish
- Make a genetic virus with these Yamanaka Factor genes to infiltrate the the cells you've extracted, using CRISPR-Cas9
- Wait a few weeks and you'll get a sizeable amount of the iPSCs (induced pluripotent stem cells)
The power of combining pluripotent stem cells with genetic/epigenetic editing
With this, you can literally modify ANYTHING about your body, like I genuinely can't think of something you can't do with this. The beautiful thing is that there is an INFINITE SUPPLY as you can continually extract from your own blood and/or skin cells.
There are risks though, if growth goes uncontrolled, the cells can turn cancerous and generate a tumor.
Also, I may or may not be mistaken about guaranteed differentiation (the stem cells turning into the intended cells). Since this is an answer I can not give you a definitive answer on, DYOR. Stem cells do differentiate into the cells they are very close to, so if you inject into your skin, they'll turn into skin cells, etc. And according to what I can see on the internet, they are very darn good at it. Your best bet is to get it as close as you can to the target area.
Some applications that I thought of with this technology combined with genetic editing (adding and removing genes) and epigenetic editing (dialing the activity of a gene up or down, think of a dial on a stove) include...
- Height: Inject these stem cells in your growth plates in a way where you can turn these stem cells into new growth plate cells, and you can grow again/have more time to grow. Bonus if you upregulate the IGF-1 gene (with CRISPRa) (a gene responsible for IGF-1 production) in the cells before injecting.
- Changing Your Ethnicity/Phenotype?: I am not too sure, this is a hypothesis on this but if you extract cells from someone of another ethnicity, your body will slowly turn into their body, as your cells' genes get slowly replaced by theirs. @Tinydragon98, escape your Filipino genes?
- Muscles: Modify the stem cells to inhibit or outright get rid of myostatin and inject into your muscles, now you can be lean/jacked w/o working out or working out less.
- Stamina: Modifying the stem cells via CRISPRa by upregulating the EPOR gene to have higher oxygen retention, meaning your physical endurance can be greatly enhanced, and inject. Imagine sprinting for two minutes straight w/o getting tired
- Eye Color Change: Modify the stem cells to downregulate (CRISPRi) or knockout (CRISPR Cas9) the OCA2 gene (one of the things this gene is responsible for is eye pigmentation depends on the eye color you are aiming for) and inject into your iris, or in your eyeballs.
- Reversing Aging and RAPID Healing anywhere: Inject into your body and you'll slowly become younger as these cells multiply in your body. They are devoid of damage, unlike your current ones. These cells are literally on par with the cells of you fresh out of the womb in terms of quality.
- Glow In The Dark Irises: Modify the stem cells and using CRISPR Cas9, add the gene for a certain fluorescent protein, ex. Blue/Green/Red Fluorescent Protein and inject SPECIFICALLY into your iris. We don't want the whites of our eyes to be glowing.
- Curing Balding: You would have the stem cells to differentiate into hair follicles, and take it to a hair transplant doctor to transplant it in.
- Changing Hair Texture: It is easier to ask someone for their blood than to genetically modify your own stem cells to change your hair texture, so again, you'd differentiate the cells into hair follicles and you'd implant them in your hair.
- Making twins of you w/o women?: My theory is that you can differentiate it into an egg, cum on it, keep it safe and implant it in a surrogate mother and it'll come out.
- And much more...
Conclusion
Thoughts? Also, don't say this isn't possible because scientists have already made iPSCs and genetically modified them.
Sources on the claim above:
Induced pluripotent stem cells meet genome editing
It is extremely rare for a single experiment to be so impactful and timely that it shapes and forecasts the experiments of the next decade. Here, we review how two such experiments --the generation of human induced pluripotent stem cells (iPSCs) and the ...
www.ncbi.nlm.nih.gov
Genetic Modification in Human Pluripotent Stem Cells by Homologous Recombination and CRISPR/Cas9 System - PubMed
Genetic modification is an indispensable tool to study gene function in normal development and disease. The recent breakthrough of creating human induced pluripotent stem cells (iPSCs) by defined factors (Takahashi et al., Cell 131:861-872, 2007) provides a renewable source of patient autologous...
pubmed.ncbi.nlm.nih.gov
Frontiers | Genome Editing in iPSC-Based Neural Systems: From Disease Models to Future Therapeutic Strategies
Therapeutic advances for neurological disorders are challenging due to limited accessibility of the human central nervous system and incomplete understanding...
www.frontiersin.org
Engineering of human induced pluripotent stem cells via human artificial chromosome vectors for cell therapy and disease modeling
Engineering of human iPSCs has great potential for cell therapy and drug discovery. Kazuki and colleagues demonstrate engineering of human iPSCs via human artificial chromosome vectors with a large cargo capacity for biomedical research, such as gene and cell therapies and generation of isogenic...
www.cell.com
Ex vivo Generation of Genetically Modified Macrophages from Human Induced Pluripotent Stem Cells
Background: Pluripotent stem cells, including induced pluripotent stem cells (iPSCs), have the capacity to differentiate towards all three germ layers and have been highlighted as an attractive cell source for the field of regenerative medicine. Thus, stable expression of therapeutic transgenes...
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