No, it doesn’t. Only synthesizing large DNA sequences costs that much. CRISPR synthesizes shorter DNA sequences, making it cheaper. I somewhat agree that it isn’t completely realistic, but I just want people to know that it is possible to do.
but hes already height maxxed
He needs to DIY more stuff. He can't be slaying what's wrong with him? It's more important to impress Dalits on .org.If anyone is gonna diy this its gonna be @Clavicular
goat.I’m 6’2 aswell
Honestly, I have no clue, and I’m not going to pretend I do. There haven’t been any human trials, so who knows? But if it actually works they will probably grow a significant amount imo
What did you send?
@enchanted_elixir thoughts? I remember you made a thread about practical applications of CRISPR, do you think this specifically is possible?Heightmaxxing Breakthrough: Activating Growth Plate Stem Cells to Grow Taller!
Alright guys, so @mathis was going to release a heightmaxxing guide, but he mentioned it was experimental and expensive. I did some digging to figure out what he could be talking about and quickly realized he was likely referring to activating growth plate stem cells to grow taller. They’ve only done it on rats so far, but the results have been really good. Since his thread is going to take a couple of weeks, I figured I’d get my thread out first:
I will drop THE BIGGEST HEIGHTMAXXING SECRET this week ( NUCLEAR THREAD )
I just discovered the biggest shit ever. You can grow ( actual TARGETED bones growth ) after puberty. Not 1 or 2 cm. 10cm easy. Just remember Its purely theory even thought it probably work you’ll need to be very careful. Some of this shit may cost a lot or may be difficult to find thought. I...looksmax.org
The Study:
View attachment 3134381
TLDR:
- Hedgehog Signaling Pathway: Activating this pathway can stimulate bone growth, but it's most effective when growth plates are still open.
- Heightmaxxing with closed growth plates: This won't work for adults with closed growth plates, as they no longer have the cartilage necessary for bone growth. While it’s a promising avenue for younger individuals with active growth plates, it won’t lead to significant height increases in adults.
In short, it will increase your height by a shit ton and it might even be possible to DIY HYPOTHETICALLY!!!
View attachment 3134376
DIY METHOD (I doubt anyone will do this it's too expensive and too complicated for most people but I just wanted to put it out there that it is possible.)
Here is everything you need to know about doing it at home. (I DO NOT CONDONE DOING THIS AT HOME. THIS IS HYPOTHETICAL.)
What is CRISPR-Cas9? (Super Important)
CRISPR-Cas9 is an incredible tool that allows scientists to make super precise edits to DNA in living cells.
- CRISPR stands for Clustered Regularly Interspaced Short Palindromic Repeats. It’s a natural defense system used by bacteria to protect themselves from viruses.
- Cas9 is an enzyme that acts like molecular scissors, cutting DNA at specific locations.
CRISPR-Cas9 Components and Cas9 Nuclease
Function:
Amount Needed:
Tools and Equipment:
Best Option:
View attachment 3134534
Plasmids
Plasmids are like the delivery dudes for gRNA and Cas9 into cells. You need to use about 1-10 µg of plasmid DNA per experiment. To make sure you’ve got the right stuff, you’ll have to use a Gel Electrophoresis Setup, which is basically a way to check if your DNA is good to go. You measure the plasmid DNA and then run it through the gel to verify it. If you’re trying not to blow all your cash, you could even build your own gel setup or hunt for cheap options online.
View attachment 3134591
Delivery Vectors
Viral vectors, like Adenovirus or Lentivirus, are used to deliver CRISPR components into cells. You’ll need 10^6 to 10^9 viral particles per mL to make it work. To purify these viral vectors, a centrifuge is required. The process involves diluting the vectors and then using the centrifuge to clean them up. If you’re trying to save some money, mini-centrifuges or second-hand equipment can be solid options.
View attachment 3134605
Study: https://pubmed.ncbi.nlm.nih.gov/26469046/
Growing Cells
To grow cells, you’ll need about 500 mL to 1 L of culture media, like DMEM or RPMI 1640, for each culture. Keeping things sterile is important, so using a Laminar Flow Hood is recommended. But if you don’t have one, setting up a clean workspace in a well-ventilated room can work too. The process is pretty simple: just pour the media into a flask, then add some Fetal Bovine Serum (FBS) and antibiotics.
View attachment 3134604
- FBS: Add 5-20% of the total media volume.
- Antibiotics (Penicillin/Streptomycin): Add 1% of the media volume to keep bacteria from messing things up.
It’s taking me too long to find a source for everything. I’m sure you can find a source somewhere for these ingredients.
Growth Factors and Chemicals
SAG (Smoothened Agonist): This stuff kicks off pathways that help cells grow. You’ll want to use a concentration of 1-10 µM. If you want to see how it’s working, you can use a Microplate Reader, but if you’re on a budget, a regular spectrophotometer works too. Just dilute the SAG and add it to your cell cultures.
IGF-1 (Recombinant Human IGF-1): This promotes cell growth and keeps them alive. You’ll need to add 10-100 ng/mL to your culture media.
Tamoxifen: This controls gene expression by hooking up with estrogen receptors. Use a concentration of 1-10 µM for this one.
SAG 1 mg | Buy Online | Thermo Scientific™ | thermofisher.com
SAG. SAG. Available in 1 mgwww.thermofisher.com
Tamoxifen, 98% 500 mg | Buy Online | Thermo Scientific Chemicals | thermofisher.com
Tamoxifen, 98%. Tamoxifen, 10540-29-1, is an antineoplastic agent that acts by competitively binding estrogen receptors to reduce DNA synthesis. Learn more at Thermo Fisher Scientific. Available in 500 mgwww.thermofisher.com
Verification and Analysis
PCR (Polymerase Chain Reaction): PCR is a technique used to make a bunch of copies of a specific DNA segment so you can analyze it. To do this, you'll need a concentration of about 0.1-1 µM and a machine called a Thermal Cycler
Sequencing (Sanger or Next-Generation): Sequencing is the method used to figure out the exact order of DNA bases, which helps you confirm any changes or edits you made. The amount of DNA you’ll need depends on which sequencing service you’re using.
View attachment 3134620
1. Getting the Solution Ready
First off, you need to mix up the solution:
- Sterilization: Make sure all your gear (like syringes, needles, and vials) is clean and sterilized to prevent contamination.
- Mixing It Up: Combine the SAG, IGF-1, and Tamoxifen in a clean vial. Give it a gentle swirl to mix everything—don’t shake it too hard, or you might mess up the mixture!
2. Filling the Syringe
Next, you’ve gotta get the solution into the syringe:
- Draw It Up: Attach the needle to the syringe and carefully draw the solution into it. Be sure to avoid any air bubbles.
- Check the Amount: Double-check that you’ve got the right amount in the syringe. Accurate dosing is crucial.
- Example Dosages: If you’re injecting 100 µL per site, ensure your mix has the correct amount of each ingredient.
3. Prepping the Injection Spot
Now, let’s get the injection site ready:
View attachment 3134721
- Keep It Clean: Perform this step in a very clean environment to avoid any contamination.
- Find the Secondary Ossification Center: While it’s best to use imaging tools like X-rays, if that’s not an option, you can locate the general area based on anatomical landmarks. The secondary ossification center is typically located near the ends of long bones, such as the femur, close to the joint. You might feel for the softer areas at the ends of bones where growth plates are located.
- Mark It: Once you’ve identified the area near the joint where the secondary ossification center is likely located, mark the spot carefully to ensure accurate injection.
4. Doing the Injection
Here’s how you do the injection:
- Clean the Area: Wipe down the marked spot with antiseptic to keep it clean.
- Stick the Needle In: Carefully insert the needle into the marked spot, aiming for the softer, slightly indented area near the joint, which is indicative of the secondary ossification center.
- Inject the Solution: Slowly inject the solution into the area. Do it steadily to ensure it goes in smoothly.
- How Much to Use: Typically, 100 µL per secondary ossification center should be sufficient, as suggested by the study. (Since you're a human and not a rat, the dosages might not be sufficient. This is all hypothetical, though, lol)
Well, this was fun to make. I doubt anyone will actually do this it's all hypothetical and costs a shit ton of money. Plus, it hasn't been tested in human trials yet, so the dosages might be off depending on your weight and other factors. I'm just going by the study, but yeah. I probably messed up a few times because I'm not a scientist, but let me know your thoughts do you think this would work on humans?
i actually read this, mirin effort and devotionHeightmaxxing Breakthrough: Activating Growth Plate Stem Cells to Grow Taller!
Alright guys, so @mathis was going to release a heightmaxxing guide, but he mentioned it was experimental and expensive. I did some digging to figure out what he could be talking about and quickly realized he was likely referring to activating growth plate stem cells to grow taller. They’ve only done it on rats so far, but the results have been really good. Since his thread is going to take a couple of weeks, I figured I’d get my thread out first:
I will drop THE BIGGEST HEIGHTMAXXING SECRET this week ( NUCLEAR THREAD )
I just discovered the biggest shit ever. You can grow ( actual TARGETED bones growth ) after puberty. Not 1 or 2 cm. 10cm easy. Just remember Its purely theory even thought it probably work you’ll need to be very careful. Some of this shit may cost a lot or may be difficult to find thought. I...
The Study:
View attachment 3134381
TLDR:
- Hedgehog Signaling Pathway: Activating this pathway can stimulate bone growth, but it's most effective when growth plates are still open.
- Heightmaxxing with closed growth plates: This won't work for adults with closed growth plates, as they no longer have the cartilage necessary for bone growth. While it’s a promising avenue for younger individuals with active growth plates, it won’t lead to significant height increases in adults.
In short, it will increase your height by a shit ton and it might even be possible to DIY HYPOTHETICALLY!!!
View attachment 3134376
DIY METHOD (I doubt anyone will do this it's too expensive and too complicated for most people but I just wanted to put it out there that it is possible.)
Here is everything you need to know about doing it at home. (I DO NOT CONDONE DOING THIS AT HOME. THIS IS HYPOTHETICAL.)
What is CRISPR-Cas9? (Super Important)
CRISPR-Cas9 is an incredible tool that allows scientists to make super precise edits to DNA in living cells.
- CRISPR stands for Clustered Regularly Interspaced Short Palindromic Repeats. It’s a natural defense system used by bacteria to protect themselves from viruses.
- Cas9 is an enzyme that acts like molecular scissors, cutting DNA at specific locations.
CRISPR-Cas9 Components and Cas9 Nuclease
Function:
Amount Needed:
Tools and Equipment:
Best Option:
View attachment 3134534
Plasmids
Plasmids are like the delivery dudes for gRNA and Cas9 into cells. You need to use about 1-10 µg of plasmid DNA per experiment. To make sure you’ve got the right stuff, you’ll have to use a Gel Electrophoresis Setup, which is basically a way to check if your DNA is good to go. You measure the plasmid DNA and then run it through the gel to verify it. If you’re trying not to blow all your cash, you could even build your own gel setup or hunt for cheap options online.
View attachment 3134591
Delivery Vectors
Viral vectors, like Adenovirus or Lentivirus, are used to deliver CRISPR components into cells. You’ll need 10^6 to 10^9 viral particles per mL to make it work. To purify these viral vectors, a centrifuge is required. The process involves diluting the vectors and then using the centrifuge to clean them up. If you’re trying to save some money, mini-centrifuges or second-hand equipment can be solid options.
View attachment 3134605
Study: https://pubmed.ncbi.nlm.nih.gov/26469046/
Growing Cells
To grow cells, you’ll need about 500 mL to 1 L of culture media, like DMEM or RPMI 1640, for each culture. Keeping things sterile is important, so using a Laminar Flow Hood is recommended. But if you don’t have one, setting up a clean workspace in a well-ventilated room can work too. The process is pretty simple: just pour the media into a flask, then add some Fetal Bovine Serum (FBS) and antibiotics.
View attachment 3134604
- FBS: Add 5-20% of the total media volume.
- Antibiotics (Penicillin/Streptomycin): Add 1% of the media volume to keep bacteria from messing things up.
It’s taking me too long to find a source for everything. I’m sure you can find a source somewhere for these ingredients.
Growth Factors and Chemicals
SAG (Smoothened Agonist): This stuff kicks off pathways that help cells grow. You’ll want to use a concentration of 1-10 µM. If you want to see how it’s working, you can use a Microplate Reader, but if you’re on a budget, a regular spectrophotometer works too. Just dilute the SAG and add it to your cell cultures.
IGF-1 (Recombinant Human IGF-1): This promotes cell growth and keeps them alive. You’ll need to add 10-100 ng/mL to your culture media.
Tamoxifen: This controls gene expression by hooking up with estrogen receptors. Use a concentration of 1-10 µM for this one.
SAG 1 mg | Buy Online | Thermo Scientific™ | thermofisher.com
SAG. SAG. Available in 1 mgwww.thermofisher.com
Tamoxifen, 98% 500 mg | Buy Online | Thermo Scientific Chemicals | thermofisher.com
Tamoxifen, 98%. Tamoxifen, 10540-29-1, is an antineoplastic agent that acts by competitively binding estrogen receptors to reduce DNA synthesis. Learn more at Thermo Fisher Scientific. Available in 500 mgwww.thermofisher.com
Verification and Analysis
PCR (Polymerase Chain Reaction): PCR is a technique used to make a bunch of copies of a specific DNA segment so you can analyze it. To do this, you'll need a concentration of about 0.1-1 µM and a machine called a Thermal Cycler
Sequencing (Sanger or Next-Generation): Sequencing is the method used to figure out the exact order of DNA bases, which helps you confirm any changes or edits you made. The amount of DNA you’ll need depends on which sequencing service you’re using.
View attachment 3134620
1. Getting the Solution Ready
First off, you need to mix up the solution:
- Sterilization: Make sure all your gear (like syringes, needles, and vials) is clean and sterilized to prevent contamination.
- Mixing It Up: Combine the SAG, IGF-1, and Tamoxifen in a clean vial. Give it a gentle swirl to mix everything—don’t shake it too hard, or you might mess up the mixture!
2. Filling the Syringe
Next, you’ve gotta get the solution into the syringe:
- Draw It Up: Attach the needle to the syringe and carefully draw the solution into it. Be sure to avoid any air bubbles.
- Check the Amount: Double-check that you’ve got the right amount in the syringe. Accurate dosing is crucial.
- Example Dosages: If you’re injecting 100 µL per site, ensure your mix has the correct amount of each ingredient.
3. Prepping the Injection Spot
Now, let’s get the injection site ready:
View attachment 3134721
- Keep It Clean: Perform this step in a very clean environment to avoid any contamination.
- Find the Secondary Ossification Center: While it’s best to use imaging tools like X-rays, if that’s not an option, you can locate the general area based on anatomical landmarks. The secondary ossification center is typically located near the ends of long bones, such as the femur, close to the joint. You might feel for the softer areas at the ends of bones where growth plates are located.
- Mark It: Once you’ve identified the area near the joint where the secondary ossification center is likely located, mark the spot carefully to ensure accurate injection.
4. Doing the Injection
Here’s how you do the injection:
- Clean the Area: Wipe down the marked spot with antiseptic to keep it clean.
- Stick the Needle In: Carefully insert the needle into the marked spot, aiming for the softer, slightly indented area near the joint, which is indicative of the secondary ossification center.
- Inject the Solution: Slowly inject the solution into the area. Do it steadily to ensure it goes in smoothly.
- How Much to Use: Typically, 100 µL per secondary ossification center should be sufficient, as suggested by the study. (Since you're a human and not a rat, the dosages might not be sufficient. This is all hypothetical, though, lol)
Well, this was fun to make. I doubt anyone will actually do this it's all hypothetical and costs a shit ton of money. Plus, it hasn't been tested in human trials yet, so the dosages might be off depending on your weight and other factors. I'm just going by the study, but yeah. I probably messed up a few times because I'm not a scientist, but let me know your thoughts do you think this would work on humans?
Good thread bumpHeightmaxxing Breakthrough: Activating Growth Plate Stem Cells to Grow Taller!
Alright guys, so @mathis was going to release a heightmaxxing guide, but he mentioned it was experimental and expensive. I did some digging to figure out what he could be talking about and quickly realized he was likely referring to activating growth plate stem cells to grow taller. They’ve only done it on rats so far, but the results have been really good. Since his thread is going to take a couple of weeks, I figured I’d get my thread out first:
I will drop THE BIGGEST HEIGHTMAXXING SECRET this week ( NUCLEAR THREAD )
I just discovered the biggest shit ever. You can grow ( actual TARGETED bones growth ) after puberty. Not 1 or 2 cm. 10cm easy. Just remember Its purely theory even thought it probably work you’ll need to be very careful. Some of this shit may cost a lot or may be difficult to find thought. I...
The Study:
View attachment 3134381
TLDR:
- Hedgehog Signaling Pathway: Activating this pathway can stimulate bone growth, but it's most effective when growth plates are still open.
- Heightmaxxing with closed growth plates: This won't work for adults with closed growth plates, as they no longer have the cartilage necessary for bone growth. While it’s a promising avenue for younger individuals with active growth plates, it won’t lead to significant height increases in adults.
In short, it will increase your height by a shit ton and it might even be possible to DIY HYPOTHETICALLY!!!
View attachment 3134376
DIY METHOD (I doubt anyone will do this it's too expensive and too complicated for most people but I just wanted to put it out there that it is possible.)
Here is everything you need to know about doing it at home. (I DO NOT CONDONE DOING THIS AT HOME. THIS IS HYPOTHETICAL.)
What is CRISPR-Cas9? (Super Important)
CRISPR-Cas9 is an incredible tool that allows scientists to make super precise edits to DNA in living cells.
- CRISPR stands for Clustered Regularly Interspaced Short Palindromic Repeats. It’s a natural defense system used by bacteria to protect themselves from viruses.
- Cas9 is an enzyme that acts like molecular scissors, cutting DNA at specific locations.
CRISPR-Cas9 Components and Cas9 Nuclease
Function:
Amount Needed:
Tools and Equipment:
Best Option:
View attachment 3134534
Plasmids
Plasmids are like the delivery dudes for gRNA and Cas9 into cells. You need to use about 1-10 µg of plasmid DNA per experiment. To make sure you’ve got the right stuff, you’ll have to use a Gel Electrophoresis Setup, which is basically a way to check if your DNA is good to go. You measure the plasmid DNA and then run it through the gel to verify it. If you’re trying not to blow all your cash, you could even build your own gel setup or hunt for cheap options online.
View attachment 3134591
Delivery Vectors
Viral vectors, like Adenovirus or Lentivirus, are used to deliver CRISPR components into cells. You’ll need 10^6 to 10^9 viral particles per mL to make it work. To purify these viral vectors, a centrifuge is required. The process involves diluting the vectors and then using the centrifuge to clean them up. If you’re trying to save some money, mini-centrifuges or second-hand equipment can be solid options.
View attachment 3134605
Study: https://pubmed.ncbi.nlm.nih.gov/26469046/
Growing Cells
To grow cells, you’ll need about 500 mL to 1 L of culture media, like DMEM or RPMI 1640, for each culture. Keeping things sterile is important, so using a Laminar Flow Hood is recommended. But if you don’t have one, setting up a clean workspace in a well-ventilated room can work too. The process is pretty simple: just pour the media into a flask, then add some Fetal Bovine Serum (FBS) and antibiotics.
View attachment 3134604
- FBS: Add 5-20% of the total media volume.
- Antibiotics (Penicillin/Streptomycin): Add 1% of the media volume to keep bacteria from messing things up.
It’s taking me too long to find a source for everything. I’m sure you can find a source somewhere for these ingredients.
Growth Factors and Chemicals
SAG (Smoothened Agonist): This stuff kicks off pathways that help cells grow. You’ll want to use a concentration of 1-10 µM. If you want to see how it’s working, you can use a Microplate Reader, but if you’re on a budget, a regular spectrophotometer works too. Just dilute the SAG and add it to your cell cultures.
IGF-1 (Recombinant Human IGF-1): This promotes cell growth and keeps them alive. You’ll need to add 10-100 ng/mL to your culture media.
Tamoxifen: This controls gene expression by hooking up with estrogen receptors. Use a concentration of 1-10 µM for this one.
SAG 1 mg | Buy Online | Thermo Scientific™ | thermofisher.com
SAG. SAG. Available in 1 mgwww.thermofisher.com
Tamoxifen, 98% 500 mg | Buy Online | Thermo Scientific Chemicals | thermofisher.com
Tamoxifen, 98%. Tamoxifen, 10540-29-1, is an antineoplastic agent that acts by competitively binding estrogen receptors to reduce DNA synthesis. Learn more at Thermo Fisher Scientific. Available in 500 mgwww.thermofisher.com
Verification and Analysis
PCR (Polymerase Chain Reaction): PCR is a technique used to make a bunch of copies of a specific DNA segment so you can analyze it. To do this, you'll need a concentration of about 0.1-1 µM and a machine called a Thermal Cycler
Sequencing (Sanger or Next-Generation): Sequencing is the method used to figure out the exact order of DNA bases, which helps you confirm any changes or edits you made. The amount of DNA you’ll need depends on which sequencing service you’re using.
View attachment 3134620
1. Getting the Solution Ready
First off, you need to mix up the solution:
- Sterilization: Make sure all your gear (like syringes, needles, and vials) is clean and sterilized to prevent contamination.
- Mixing It Up: Combine the SAG, IGF-1, and Tamoxifen in a clean vial. Give it a gentle swirl to mix everything—don’t shake it too hard, or you might mess up the mixture!
2. Filling the Syringe
Next, you’ve gotta get the solution into the syringe:
- Draw It Up: Attach the needle to the syringe and carefully draw the solution into it. Be sure to avoid any air bubbles.
- Check the Amount: Double-check that you’ve got the right amount in the syringe. Accurate dosing is crucial.
- Example Dosages: If you’re injecting 100 µL per site, ensure your mix has the correct amount of each ingredient.
3. Prepping the Injection Spot
Now, let’s get the injection site ready:
View attachment 3134721
- Keep It Clean: Perform this step in a very clean environment to avoid any contamination.
- Find the Secondary Ossification Center: While it’s best to use imaging tools like X-rays, if that’s not an option, you can locate the general area based on anatomical landmarks. The secondary ossification center is typically located near the ends of long bones, such as the femur, close to the joint. You might feel for the softer areas at the ends of bones where growth plates are located.
- Mark It: Once you’ve identified the area near the joint where the secondary ossification center is likely located, mark the spot carefully to ensure accurate injection.
4. Doing the Injection
Here’s how you do the injection:
- Clean the Area: Wipe down the marked spot with antiseptic to keep it clean.
- Stick the Needle In: Carefully insert the needle into the marked spot, aiming for the softer, slightly indented area near the joint, which is indicative of the secondary ossification center.
- Inject the Solution: Slowly inject the solution into the area. Do it steadily to ensure it goes in smoothly.
- How Much to Use: Typically, 100 µL per secondary ossification center should be sufficient, as suggested by the study. (Since you're a human and not a rat, the dosages might not be sufficient. This is all hypothetical, though, lol)
Well, this was fun to make. I doubt anyone will actually do this it's all hypothetical and costs a shit ton of money. Plus, it hasn't been tested in human trials yet, so the dosages might be off depending on your weight and other factors. I'm just going by the study, but yeah. I probably messed up a few times because I'm not a scientist, but let me know your thoughts do you think this would work on humans?
I admire your dedication to the topic of height growthHeightmaxxing Breakthrough: Activating Growth Plate Stem Cells to Grow Taller!
Alright guys, so @mathis was going to release a heightmaxxing guide, but he mentioned it was experimental and expensive. I did some digging to figure out what he could be talking about and quickly realized he was likely referring to activating growth plate stem cells to grow taller. They’ve only done it on rats so far, but the results have been really good. Since his thread is going to take a couple of weeks, I figured I’d get my thread out first:
I will drop THE BIGGEST HEIGHTMAXXING SECRET this week ( NUCLEAR THREAD )
I just discovered the biggest shit ever. You can grow ( actual TARGETED bones growth ) after puberty. Not 1 or 2 cm. 10cm easy. Just remember Its purely theory even thought it probably work you’ll need to be very careful. Some of this shit may cost a lot or may be difficult to find thought. I...
The Study:
View attachment 3134381
TLDR:
- Hedgehog Signaling Pathway: Activating this pathway can stimulate bone growth, but it's most effective when growth plates are still open.
- Heightmaxxing with closed growth plates: This won't work for adults with closed growth plates, as they no longer have the cartilage necessary for bone growth. While it’s a promising avenue for younger individuals with active growth plates, it won’t lead to significant height increases in adults.
In short, it will increase your height by a shit ton and it might even be possible to DIY HYPOTHETICALLY!!!
View attachment 3134376
DIY METHOD (I doubt anyone will do this it's too expensive and too complicated for most people but I just wanted to put it out there that it is possible.)
Here is everything you need to know about doing it at home. (I DO NOT CONDONE DOING THIS AT HOME. THIS IS HYPOTHETICAL.)
What is CRISPR-Cas9? (Super Important)
CRISPR-Cas9 is an incredible tool that allows scientists to make super precise edits to DNA in living cells.
- CRISPR stands for Clustered Regularly Interspaced Short Palindromic Repeats. It’s a natural defense system used by bacteria to protect themselves from viruses.
- Cas9 is an enzyme that acts like molecular scissors, cutting DNA at specific locations.
CRISPR-Cas9 Components and Cas9 Nuclease
Function:
Amount Needed:
Tools and Equipment:
Best Option:
View attachment 3134534
Plasmids
Plasmids are like the delivery dudes for gRNA and Cas9 into cells. You need to use about 1-10 µg of plasmid DNA per experiment. To make sure you’ve got the right stuff, you’ll have to use a Gel Electrophoresis Setup, which is basically a way to check if your DNA is good to go. You measure the plasmid DNA and then run it through the gel to verify it. If you’re trying not to blow all your cash, you could even build your own gel setup or hunt for cheap options online.
View attachment 3134591
Delivery Vectors
Viral vectors, like Adenovirus or Lentivirus, are used to deliver CRISPR components into cells. You’ll need 10^6 to 10^9 viral particles per mL to make it work. To purify these viral vectors, a centrifuge is required. The process involves diluting the vectors and then using the centrifuge to clean them up. If you’re trying to save some money, mini-centrifuges or second-hand equipment can be solid options.
View attachment 3134605
Study: https://pubmed.ncbi.nlm.nih.gov/26469046/
Growing Cells
To grow cells, you’ll need about 500 mL to 1 L of culture media, like DMEM or RPMI 1640, for each culture. Keeping things sterile is important, so using a Laminar Flow Hood is recommended. But if you don’t have one, setting up a clean workspace in a well-ventilated room can work too. The process is pretty simple: just pour the media into a flask, then add some Fetal Bovine Serum (FBS) and antibiotics.
View attachment 3134604
- FBS: Add 5-20% of the total media volume.
- Antibiotics (Penicillin/Streptomycin): Add 1% of the media volume to keep bacteria from messing things up.
It’s taking me too long to find a source for everything. I’m sure you can find a source somewhere for these ingredients.
Growth Factors and Chemicals
SAG (Smoothened Agonist): This stuff kicks off pathways that help cells grow. You’ll want to use a concentration of 1-10 µM. If you want to see how it’s working, you can use a Microplate Reader, but if you’re on a budget, a regular spectrophotometer works too. Just dilute the SAG and add it to your cell cultures.
IGF-1 (Recombinant Human IGF-1): This promotes cell growth and keeps them alive. You’ll need to add 10-100 ng/mL to your culture media.
Tamoxifen: This controls gene expression by hooking up with estrogen receptors. Use a concentration of 1-10 µM for this one.
SAG 1 mg | Buy Online | Thermo Scientific™ | thermofisher.com
SAG. SAG. Available in 1 mgwww.thermofisher.com
Tamoxifen, 98% 500 mg | Buy Online | Thermo Scientific Chemicals | thermofisher.com
Tamoxifen, 98%. Tamoxifen, 10540-29-1, is an antineoplastic agent that acts by competitively binding estrogen receptors to reduce DNA synthesis. Learn more at Thermo Fisher Scientific. Available in 500 mgwww.thermofisher.com
Verification and Analysis
PCR (Polymerase Chain Reaction): PCR is a technique used to make a bunch of copies of a specific DNA segment so you can analyze it. To do this, you'll need a concentration of about 0.1-1 µM and a machine called a Thermal Cycler
Sequencing (Sanger or Next-Generation): Sequencing is the method used to figure out the exact order of DNA bases, which helps you confirm any changes or edits you made. The amount of DNA you’ll need depends on which sequencing service you’re using.
View attachment 3134620
1. Getting the Solution Ready
First off, you need to mix up the solution:
- Sterilization: Make sure all your gear (like syringes, needles, and vials) is clean and sterilized to prevent contamination.
- Mixing It Up: Combine the SAG, IGF-1, and Tamoxifen in a clean vial. Give it a gentle swirl to mix everything—don’t shake it too hard, or you might mess up the mixture!
2. Filling the Syringe
Next, you’ve gotta get the solution into the syringe:
- Draw It Up: Attach the needle to the syringe and carefully draw the solution into it. Be sure to avoid any air bubbles.
- Check the Amount: Double-check that you’ve got the right amount in the syringe. Accurate dosing is crucial.
- Example Dosages: If you’re injecting 100 µL per site, ensure your mix has the correct amount of each ingredient.
3. Prepping the Injection Spot
Now, let’s get the injection site ready:
View attachment 3134721
- Keep It Clean: Perform this step in a very clean environment to avoid any contamination.
- Find the Secondary Ossification Center: While it’s best to use imaging tools like X-rays, if that’s not an option, you can locate the general area based on anatomical landmarks. The secondary ossification center is typically located near the ends of long bones, such as the femur, close to the joint. You might feel for the softer areas at the ends of bones where growth plates are located.
- Mark It: Once you’ve identified the area near the joint where the secondary ossification center is likely located, mark the spot carefully to ensure accurate injection.
4. Doing the Injection
Here’s how you do the injection:
- Clean the Area: Wipe down the marked spot with antiseptic to keep it clean.
- Stick the Needle In: Carefully insert the needle into the marked spot, aiming for the softer, slightly indented area near the joint, which is indicative of the secondary ossification center.
- Inject the Solution: Slowly inject the solution into the area. Do it steadily to ensure it goes in smoothly.
- How Much to Use: Typically, 100 µL per secondary ossification center should be sufficient, as suggested by the study. (Since you're a human and not a rat, the dosages might not be sufficient. This is all hypothetical, though, lol)
Well, this was fun to make. I doubt anyone will do this it's all hypothetical and costs a shit ton of money. Plus, it hasn't been tested in human trials yet, so the dosages might be off depending on your weight and other factors. I'm just going by the study, but yeah. I probably messed up a few times because I'm not a scientist, but let me know your thoughts do you think this would work on humans?
This better be good bhai
This is not what you imagine @mathis 's topic will be about
i swear bones usually fuse in ascending order from feet up
Not possible yet. If it’s being done on animals now estimate 3-5 years before humansHeightmaxxing Breakthrough: Activating Growth Plate Stem Cells to Grow Taller!
Alright guys, so @mathis was going to release a heightmaxxing guide, but he mentioned it was experimental and expensive. I did some digging to figure out what he could be talking about and quickly realized he was likely referring to activating growth plate stem cells to grow taller. They’ve only done it on rats so far, but the results have been really good. Since his thread is going to take a couple of weeks, I figured I’d get my thread out first:
I will drop THE BIGGEST HEIGHTMAXXING SECRET this week ( NUCLEAR THREAD )
I just discovered the biggest shit ever. You can grow ( actual TARGETED bones growth ) after puberty. Not 1 or 2 cm. 10cm easy. Just remember Its purely theory even thought it probably work you’ll need to be very careful. Some of this shit may cost a lot or may be difficult to find thought. I...
The Study:
View attachment 3134381
TLDR:
- Hedgehog Signaling Pathway: Activating this pathway can stimulate bone growth, but it's most effective when growth plates are still open.
- Heightmaxxing with closed growth plates: This won't work for adults with closed growth plates, as they no longer have the cartilage necessary for bone growth. While it’s a promising avenue for younger individuals with active growth plates, it won’t lead to significant height increases in adults.
In short, it will increase your height by a shit ton and it might even be possible to DIY HYPOTHETICALLY!!!
View attachment 3134376
DIY METHOD (I doubt anyone will do this it's too expensive and too complicated for most people but I just wanted to put it out there that it is possible.)
Here is everything you need to know about doing it at home. (I DO NOT CONDONE DOING THIS AT HOME. THIS IS HYPOTHETICAL.)
What is CRISPR-Cas9? (Super Important)
CRISPR-Cas9 is an incredible tool that allows scientists to make super precise edits to DNA in living cells.
- CRISPR stands for Clustered Regularly Interspaced Short Palindromic Repeats. It’s a natural defense system used by bacteria to protect themselves from viruses.
- Cas9 is an enzyme that acts like molecular scissors, cutting DNA at specific locations.
CRISPR-Cas9 Components and Cas9 Nuclease
Function:
Amount Needed:
Tools and Equipment:
Best Option:
View attachment 3134534
Plasmids
Plasmids are like the delivery dudes for gRNA and Cas9 into cells. You need to use about 1-10 µg of plasmid DNA per experiment. To make sure you’ve got the right stuff, you’ll have to use a Gel Electrophoresis Setup, which is basically a way to check if your DNA is good to go. You measure the plasmid DNA and then run it through the gel to verify it. If you’re trying not to blow all your cash, you could even build your own gel setup or hunt for cheap options online.
View attachment 3134591
Delivery Vectors
Viral vectors, like Adenovirus or Lentivirus, are used to deliver CRISPR components into cells. You’ll need 10^6 to 10^9 viral particles per mL to make it work. To purify these viral vectors, a centrifuge is required. The process involves diluting the vectors and then using the centrifuge to clean them up. If you’re trying to save some money, mini-centrifuges or second-hand equipment can be solid options.
View attachment 3134605
Study: https://pubmed.ncbi.nlm.nih.gov/26469046/
Growing Cells
To grow cells, you’ll need about 500 mL to 1 L of culture media, like DMEM or RPMI 1640, for each culture. Keeping things sterile is important, so using a Laminar Flow Hood is recommended. But if you don’t have one, setting up a clean workspace in a well-ventilated room can work too. The process is pretty simple: just pour the media into a flask, then add some Fetal Bovine Serum (FBS) and antibiotics.
View attachment 3134604
- FBS: Add 5-20% of the total media volume.
- Antibiotics (Penicillin/Streptomycin): Add 1% of the media volume to keep bacteria from messing things up.
It’s taking me too long to find a source for everything. I’m sure you can find a source somewhere for these ingredients.
Growth Factors and Chemicals
SAG (Smoothened Agonist): This stuff kicks off pathways that help cells grow. You’ll want to use a concentration of 1-10 µM. If you want to see how it’s working, you can use a Microplate Reader, but if you’re on a budget, a regular spectrophotometer works too. Just dilute the SAG and add it to your cell cultures.
IGF-1 (Recombinant Human IGF-1): This promotes cell growth and keeps them alive. You’ll need to add 10-100 ng/mL to your culture media.
Tamoxifen: This controls gene expression by hooking up with estrogen receptors. Use a concentration of 1-10 µM for this one.
SAG 1 mg | Buy Online | Thermo Scientific™ | thermofisher.com
SAG. SAG. Available in 1 mgwww.thermofisher.com
Tamoxifen, 98% 500 mg | Buy Online | Thermo Scientific Chemicals | thermofisher.com
Tamoxifen, 98%. Tamoxifen, 10540-29-1, is an antineoplastic agent that acts by competitively binding estrogen receptors to reduce DNA synthesis. Learn more at Thermo Fisher Scientific. Available in 500 mgwww.thermofisher.com
Verification and Analysis
PCR (Polymerase Chain Reaction): PCR is a technique used to make a bunch of copies of a specific DNA segment so you can analyze it. To do this, you'll need a concentration of about 0.1-1 µM and a machine called a Thermal Cycler
Sequencing (Sanger or Next-Generation): Sequencing is the method used to figure out the exact order of DNA bases, which helps you confirm any changes or edits you made. The amount of DNA you’ll need depends on which sequencing service you’re using.
View attachment 3134620
1. Getting the Solution Ready
First off, you need to mix up the solution:
- Sterilization: Make sure all your gear (like syringes, needles, and vials) is clean and sterilized to prevent contamination.
- Mixing It Up: Combine the SAG, IGF-1, and Tamoxifen in a clean vial. Give it a gentle swirl to mix everything—don’t shake it too hard, or you might mess up the mixture!
2. Filling the Syringe
Next, you’ve gotta get the solution into the syringe:
- Draw It Up: Attach the needle to the syringe and carefully draw the solution into it. Be sure to avoid any air bubbles.
- Check the Amount: Double-check that you’ve got the right amount in the syringe. Accurate dosing is crucial.
- Example Dosages: If you’re injecting 100 µL per site, ensure your mix has the correct amount of each ingredient.
3. Prepping the Injection Spot
Now, let’s get the injection site ready:
View attachment 3134721
- Keep It Clean: Perform this step in a very clean environment to avoid any contamination.
- Find the Secondary Ossification Center: While it’s best to use imaging tools like X-rays, if that’s not an option, you can locate the general area based on anatomical landmarks. The secondary ossification center is typically located near the ends of long bones, such as the femur, close to the joint. You might feel for the softer areas at the ends of bones where growth plates are located.
- Mark It: Once you’ve identified the area near the joint where the secondary ossification center is likely located, mark the spot carefully to ensure accurate injection.
4. Doing the Injection
Here’s how you do the injection:
- Clean the Area: Wipe down the marked spot with antiseptic to keep it clean.
- Stick the Needle In: Carefully insert the needle into the marked spot, aiming for the softer, slightly indented area near the joint, which is indicative of the secondary ossification center.
- Inject the Solution: Slowly inject the solution into the area. Do it steadily to ensure it goes in smoothly.
- How Much to Use: Typically, 100 µL per secondary ossification center should be sufficient, as suggested by the study. (Since you're a human and not a rat, the dosages might not be sufficient. This is all hypothetical, though, lol)
Well, this was fun to make. I doubt anyone will actually do this it's all hypothetical and costs a shit ton of money. Plus, it hasn't been tested in human trials yet, so the dosages might be off depending on your weight and other factors. I'm just going by the study, but yeah. I probably messed up a few times because I'm not a scientist, but let me know your thoughts do you think this would work on humans?
I'm sorry about that yeah, you're right.
You'd need to be like a graduate student or a Ph.D.
Good luck. You're going to have to make sure you epigenetically signal HH really high, and do that perfectly.Heightmaxxing Breakthrough: Activating Growth Plate Stem Cells to Grow Taller!
Alright guys, so @mathis was going to release a heightmaxxing guide, but he mentioned it was experimental and expensive. I did some digging to figure out what he could be talking about and quickly realized he was likely referring to activating growth plate stem cells to grow taller. They’ve only done it on rats so far, but the results have been really good. Since his thread is going to take a couple of weeks, I figured I’d get my thread out first:
I will drop THE BIGGEST HEIGHTMAXXING SECRET this week ( NUCLEAR THREAD )
I just discovered the biggest shit ever. You can grow ( actual TARGETED bones growth ) after puberty. Not 1 or 2 cm. 10cm easy. Just remember Its purely theory even thought it probably work you’ll need to be very careful. Some of this shit may cost a lot or may be difficult to find thought. I...
The Study:
View attachment 3134381
TLDR:
- Hedgehog Signaling Pathway: Activating this pathway can stimulate bone growth, but it's most effective when growth plates are still open.
- Heightmaxxing with closed growth plates: This won't work for adults with closed growth plates, as they no longer have the cartilage necessary for bone growth. While it’s a promising avenue for younger individuals with active growth plates, it won’t lead to significant height increases in adults.
In short, it will increase your height by a shit ton and it might even be possible to DIY HYPOTHETICALLY!!!
View attachment 3134376
DIY METHOD (I doubt anyone will do this it's too expensive and too complicated for most people but I just wanted to put it out there that it is possible.)
Here is everything you need to know about doing it at home. (I DO NOT CONDONE DOING THIS AT HOME. THIS IS HYPOTHETICAL.)
What is CRISPR-Cas9? (Super Important)
CRISPR-Cas9 is an incredible tool that allows scientists to make super precise edits to DNA in living cells.
- CRISPR stands for Clustered Regularly Interspaced Short Palindromic Repeats. It’s a natural defense system used by bacteria to protect themselves from viruses.
- Cas9 is an enzyme that acts like molecular scissors, cutting DNA at specific locations.
CRISPR-Cas9 Components and Cas9 Nuclease
Function:
Amount Needed:
Tools and Equipment:
Best Option:
View attachment 3134534
Plasmids
Plasmids are like the delivery dudes for gRNA and Cas9 into cells. You need to use about 1-10 µg of plasmid DNA per experiment. To make sure you’ve got the right stuff, you’ll have to use a Gel Electrophoresis Setup, which is basically a way to check if your DNA is good to go. You measure the plasmid DNA and then run it through the gel to verify it. If you’re trying not to blow all your cash, you could even build your own gel setup or hunt for cheap options online.
View attachment 3134591
Delivery Vectors
Viral vectors, like Adenovirus or Lentivirus, are used to deliver CRISPR components into cells. You’ll need 10^6 to 10^9 viral particles per mL to make it work. To purify these viral vectors, a centrifuge is required. The process involves diluting the vectors and then using the centrifuge to clean them up. If you’re trying to save some money, mini-centrifuges or second-hand equipment can be solid options.
View attachment 3134605
Study: https://pubmed.ncbi.nlm.nih.gov/26469046/
Growing Cells
To grow cells, you’ll need about 500 mL to 1 L of culture media, like DMEM or RPMI 1640, for each culture. Keeping things sterile is important, so using a Laminar Flow Hood is recommended. But if you don’t have one, setting up a clean workspace in a well-ventilated room can work too. The process is pretty simple: just pour the media into a flask, then add some Fetal Bovine Serum (FBS) and antibiotics.
View attachment 3134604
- FBS: Add 5-20% of the total media volume.
- Antibiotics (Penicillin/Streptomycin): Add 1% of the media volume to keep bacteria from messing things up.
It’s taking me too long to find a source for everything. I’m sure you can find a source somewhere for these ingredients.
Growth Factors and Chemicals
SAG (Smoothened Agonist): This stuff kicks off pathways that help cells grow. You’ll want to use a concentration of 1-10 µM. If you want to see how it’s working, you can use a Microplate Reader, but if you’re on a budget, a regular spectrophotometer works too. Just dilute the SAG and add it to your cell cultures.
IGF-1 (Recombinant Human IGF-1): This promotes cell growth and keeps them alive. You’ll need to add 10-100 ng/mL to your culture media.
Tamoxifen: This controls gene expression by hooking up with estrogen receptors. Use a concentration of 1-10 µM for this one.
SAG 1 mg | Buy Online | Thermo Scientific™ | thermofisher.com
SAG. SAG. Available in 1 mgwww.thermofisher.com
Tamoxifen, 98% 500 mg | Buy Online | Thermo Scientific Chemicals | thermofisher.com
Tamoxifen, 98%. Tamoxifen, 10540-29-1, is an antineoplastic agent that acts by competitively binding estrogen receptors to reduce DNA synthesis. Learn more at Thermo Fisher Scientific. Available in 500 mgwww.thermofisher.com
Verification and Analysis
PCR (Polymerase Chain Reaction): PCR is a technique used to make a bunch of copies of a specific DNA segment so you can analyze it. To do this, you'll need a concentration of about 0.1-1 µM and a machine called a Thermal Cycler
Sequencing (Sanger or Next-Generation): Sequencing is the method used to figure out the exact order of DNA bases, which helps you confirm any changes or edits you made. The amount of DNA you’ll need depends on which sequencing service you’re using.
View attachment 3134620
1. Getting the Solution Ready
First off, you need to mix up the solution:
- Sterilization: Make sure all your gear (like syringes, needles, and vials) is clean and sterilized to prevent contamination.
- Mixing It Up: Combine the SAG, IGF-1, and Tamoxifen in a clean vial. Give it a gentle swirl to mix everything—don’t shake it too hard, or you might mess up the mixture!
2. Filling the Syringe
Next, you’ve gotta get the solution into the syringe:
- Draw It Up: Attach the needle to the syringe and carefully draw the solution into it. Be sure to avoid any air bubbles.
- Check the Amount: Double-check that you’ve got the right amount in the syringe. Accurate dosing is crucial.
- Example Dosages: If you’re injecting 100 µL per site, ensure your mix has the correct amount of each ingredient.
3. Prepping the Injection Spot
Now, let’s get the injection site ready:
View attachment 3134721
- Keep It Clean: Perform this step in a very clean environment to avoid any contamination.
- Find the Secondary Ossification Center: While it’s best to use imaging tools like X-rays, if that’s not an option, you can locate the general area based on anatomical landmarks. The secondary ossification center is typically located near the ends of long bones, such as the femur, close to the joint. You might feel for the softer areas at the ends of bones where growth plates are located.
- Mark It: Once you’ve identified the area near the joint where the secondary ossification center is likely located, mark the spot carefully to ensure accurate injection.
4. Doing the Injection
Here’s how you do the injection:
- Clean the Area: Wipe down the marked spot with antiseptic to keep it clean.
- Stick the Needle In: Carefully insert the needle into the marked spot, aiming for the softer, slightly indented area near the joint, which is indicative of the secondary ossification center.
- Inject the Solution: Slowly inject the solution into the area. Do it steadily to ensure it goes in smoothly.
- How Much to Use: Typically, 100 µL per secondary ossification center should be sufficient, as suggested by the study. (Since you're a human and not a rat, the dosages might not be sufficient. This is all hypothetical, though, lol)
Well, this was fun to make. I doubt anyone will actually do this it's all hypothetical and costs a shit ton of money. Plus, it hasn't been tested in human trials yet, so the dosages might be off depending on your weight and other factors. I'm just going by the study, but yeah. I probably messed up a few times because I'm not a scientist, but let me know your thoughts do you think this would work on humans?
I've done gel electrophoresis in lab at my college.+1 rep
ive studied genetics so what you're talking about is true
but like you said it is very costly, not only that but the average person will have much difficulty replicating it
i struggled to do basic gel electropheresis lol
good thread
They're like 20 us cents per base pair maximum
This probably works better for clavicles if it does work.
lifefuel if anything works for oldcels like me
You need active growth plates.
Feel bad for @mathis, elite thread though.Heightmaxxing Breakthrough: Activating Growth Plate Stem Cells to Grow Taller!
Alright guys, so @mathis was going to release a heightmaxxing guide, but he mentioned it was experimental and expensive. I did some digging to figure out what he could be talking about and quickly realized he was likely referring to activating growth plate stem cells to grow taller. They’ve only done it on rats so far, but the results have been really good. Since his thread is going to take a couple of weeks, I figured I’d get my thread out first:
I will drop THE BIGGEST HEIGHTMAXXING SECRET this week ( NUCLEAR THREAD )
I just discovered the biggest shit ever. You can grow ( actual TARGETED bones growth ) after puberty. Not 1 or 2 cm. 10cm easy. Just remember Its purely theory even thought it probably work you’ll need to be very careful. Some of this shit may cost a lot or may be difficult to find thought. I...
The Study:
View attachment 3134381
TLDR:
- Hedgehog Signaling Pathway: Activating this pathway can stimulate bone growth, but it's most effective when growth plates are still open.
- Heightmaxxing with closed growth plates: This won't work for adults with closed growth plates, as they no longer have the cartilage necessary for bone growth. While it’s a promising avenue for younger individuals with active growth plates, it won’t lead to significant height increases in adults.
In short, it will increase your height by a shit ton and it might even be possible to DIY HYPOTHETICALLY!!!
View attachment 3134376
DIY METHOD (I doubt anyone will do this it's too expensive and too complicated for most people but I just wanted to put it out there that it is possible.)
Here is everything you need to know about doing it at home. (I DO NOT CONDONE DOING THIS AT HOME. THIS IS HYPOTHETICAL.)
What is CRISPR-Cas9? (Super Important)
CRISPR-Cas9 is an incredible tool that allows scientists to make super precise edits to DNA in living cells.
- CRISPR stands for Clustered Regularly Interspaced Short Palindromic Repeats. It’s a natural defense system used by bacteria to protect themselves from viruses.
- Cas9 is an enzyme that acts like molecular scissors, cutting DNA at specific locations.
CRISPR-Cas9 Components and Cas9 Nuclease
Function:
Amount Needed:
Tools and Equipment:
Best Option:
View attachment 3134534
Plasmids
Plasmids are like the delivery dudes for gRNA and Cas9 into cells. You need to use about 1-10 µg of plasmid DNA per experiment. To make sure you’ve got the right stuff, you’ll have to use a Gel Electrophoresis Setup, which is basically a way to check if your DNA is good to go. You measure the plasmid DNA and then run it through the gel to verify it. If you’re trying not to blow all your cash, you could even build your own gel setup or hunt for cheap options online.
View attachment 3134591
Delivery Vectors
Viral vectors, like Adenovirus or Lentivirus, are used to deliver CRISPR components into cells. You’ll need 10^6 to 10^9 viral particles per mL to make it work. To purify these viral vectors, a centrifuge is required. The process involves diluting the vectors and then using the centrifuge to clean them up. If you’re trying to save some money, mini-centrifuges or second-hand equipment can be solid options.
View attachment 3134605
Study: https://pubmed.ncbi.nlm.nih.gov/26469046/
Growing Cells
To grow cells, you’ll need about 500 mL to 1 L of culture media, like DMEM or RPMI 1640, for each culture. Keeping things sterile is important, so using a Laminar Flow Hood is recommended. But if you don’t have one, setting up a clean workspace in a well-ventilated room can work too. The process is pretty simple: just pour the media into a flask, then add some Fetal Bovine Serum (FBS) and antibiotics.
View attachment 3134604
- FBS: Add 5-20% of the total media volume.
- Antibiotics (Penicillin/Streptomycin): Add 1% of the media volume to keep bacteria from messing things up.
It’s taking me too long to find a source for everything. I’m sure you can find a source somewhere for these ingredients.
Growth Factors and Chemicals
SAG (Smoothened Agonist): This stuff kicks off pathways that help cells grow. You’ll want to use a concentration of 1-10 µM. If you want to see how it’s working, you can use a Microplate Reader, but if you’re on a budget, a regular spectrophotometer works too. Just dilute the SAG and add it to your cell cultures.
IGF-1 (Recombinant Human IGF-1): This promotes cell growth and keeps them alive. You’ll need to add 10-100 ng/mL to your culture media.
Tamoxifen: This controls gene expression by hooking up with estrogen receptors. Use a concentration of 1-10 µM for this one.
SAG 1 mg | Buy Online | Thermo Scientific™ | thermofisher.com
SAG. SAG. Available in 1 mgwww.thermofisher.com
Tamoxifen, 98% 500 mg | Buy Online | Thermo Scientific Chemicals | thermofisher.com
Tamoxifen, 98%. Tamoxifen, 10540-29-1, is an antineoplastic agent that acts by competitively binding estrogen receptors to reduce DNA synthesis. Learn more at Thermo Fisher Scientific. Available in 500 mgwww.thermofisher.com
Verification and Analysis
PCR (Polymerase Chain Reaction): PCR is a technique used to make a bunch of copies of a specific DNA segment so you can analyze it. To do this, you'll need a concentration of about 0.1-1 µM and a machine called a Thermal Cycler
Sequencing (Sanger or Next-Generation): Sequencing is the method used to figure out the exact order of DNA bases, which helps you confirm any changes or edits you made. The amount of DNA you’ll need depends on which sequencing service you’re using.
View attachment 3134620
1. Getting the Solution Ready
First off, you need to mix up the solution:
- Sterilization: Make sure all your gear (like syringes, needles, and vials) is clean and sterilized to prevent contamination.
- Mixing It Up: Combine the SAG, IGF-1, and Tamoxifen in a clean vial. Give it a gentle swirl to mix everything—don’t shake it too hard, or you might mess up the mixture!
2. Filling the Syringe
Next, you’ve gotta get the solution into the syringe:
- Draw It Up: Attach the needle to the syringe and carefully draw the solution into it. Be sure to avoid any air bubbles.
- Check the Amount: Double-check that you’ve got the right amount in the syringe. Accurate dosing is crucial.
- Example Dosages: If you’re injecting 100 µL per site, ensure your mix has the correct amount of each ingredient.
3. Prepping the Injection Spot
Now, let’s get the injection site ready:
View attachment 3134721
- Keep It Clean: Perform this step in a very clean environment to avoid any contamination.
- Find the Secondary Ossification Center: While it’s best to use imaging tools like X-rays, if that’s not an option, you can locate the general area based on anatomical landmarks. The secondary ossification center is typically located near the ends of long bones, such as the femur, close to the joint. You might feel for the softer areas at the ends of bones where growth plates are located.
- Mark It: Once you’ve identified the area near the joint where the secondary ossification center is likely located, mark the spot carefully to ensure accurate injection.
4. Doing the Injection
Here’s how you do the injection:
- Clean the Area: Wipe down the marked spot with antiseptic to keep it clean.
- Stick the Needle In: Carefully insert the needle into the marked spot, aiming for the softer, slightly indented area near the joint, which is indicative of the secondary ossification center.
- Inject the Solution: Slowly inject the solution into the area. Do it steadily to ensure it goes in smoothly.
- How Much to Use: Typically, 100 µL per secondary ossification center should be sufficient, as suggested by the study. (Since you're a human and not a rat, the dosages might not be sufficient. This is all hypothetical, though, lol)
Well, this was fun to make. I doubt anyone will actually do this it's all hypothetical and costs a shit ton of money. Plus, it hasn't been tested in human trials yet, so the dosages might be off depending on your weight and other factors. I'm just going by the study, but yeah. I probably messed up a few times because I'm not a scientist, but let me know your thoughts do you think this would work on humans?
Yep, that's right. They used Tamoxifen in this study to control genetic changes in the animal subjects, rather than for its usual effects on bone tissue. I just stuck to what the study said and didn’t want to change anything in case I was wrong.
Yes, that was what I said at the beginning.
I was replying to someone else.
Stopped reading after this
Yes ik it’s risky and complicated, which is why I don't expect anyone to actually do it. But what would be the best way to go about it to make sure you do this correctly, in your opinion?
I can't say for sure since I don't even have an associates degree yet.
There should be a life maxing server where we all collectively try to figure out how to live forever and stop the agepill
Should we make this ?
We'd need better minds in that chat.
Dndr but rep for the time this tookHeightmaxxing Breakthrough: Activating Growth Plate Stem Cells to Grow Taller!
Alright guys, so @mathis was going to release a heightmaxxing guide, but he mentioned it was experimental and expensive. I did some digging to figure out what he could be talking about and quickly realized he was likely referring to activating growth plate stem cells to grow taller. They’ve only done it on rats so far, but the results have been really good. Since his thread is going to take a couple of weeks, I figured I’d get my thread out first:
I will drop THE BIGGEST HEIGHTMAXXING SECRET this week ( NUCLEAR THREAD )
I just discovered the biggest shit ever. You can grow ( actual TARGETED bones growth ) after puberty. Not 1 or 2 cm. 10cm easy. Just remember Its purely theory even thought it probably work you’ll need to be very careful. Some of this shit may cost a lot or may be difficult to find thought. I...
The Study:
View attachment 3134381
TLDR:
- Hedgehog Signaling Pathway: Activating this pathway can stimulate bone growth, but it's most effective when growth plates are still open.
- Heightmaxxing with closed growth plates: This won't work for adults with closed growth plates, as they no longer have the cartilage necessary for bone growth. While it’s a promising avenue for younger individuals with active growth plates, it won’t lead to significant height increases in adults.
In short, it will increase your height by a shit ton and it might even be possible to DIY HYPOTHETICALLY!!!
View attachment 3134376
DIY METHOD (I doubt anyone will do this it's too expensive and too complicated for most people but I just wanted to put it out there that it is possible.)
Here is everything you need to know about doing it at home. (I DO NOT CONDONE DOING THIS AT HOME. THIS IS HYPOTHETICAL.)
What is CRISPR-Cas9? (Super Important)
CRISPR-Cas9 is an incredible tool that allows scientists to make super precise edits to DNA in living cells.
- CRISPR stands for Clustered Regularly Interspaced Short Palindromic Repeats. It’s a natural defense system used by bacteria to protect themselves from viruses.
- Cas9 is an enzyme that acts like molecular scissors, cutting DNA at specific locations.
CRISPR-Cas9 Components and Cas9 Nuclease
Function:
Amount Needed:
Tools and Equipment:
Best Option:
View attachment 3134534
Plasmids
Plasmids are like the delivery dudes for gRNA and Cas9 into cells. You need to use about 1-10 µg of plasmid DNA per experiment. To make sure you’ve got the right stuff, you’ll have to use a Gel Electrophoresis Setup, which is basically a way to check if your DNA is good to go. You measure the plasmid DNA and then run it through the gel to verify it. If you’re trying not to blow all your cash, you could even build your own gel setup or hunt for cheap options online.
View attachment 3134591
Delivery Vectors
Viral vectors, like Adenovirus or Lentivirus, are used to deliver CRISPR components into cells. You’ll need 10^6 to 10^9 viral particles per mL to make it work. To purify these viral vectors, a centrifuge is required. The process involves diluting the vectors and then using the centrifuge to clean them up. If you’re trying to save some money, mini-centrifuges or second-hand equipment can be solid options.
View attachment 3134605
Study: https://pubmed.ncbi.nlm.nih.gov/26469046/
Growing Cells
To grow cells, you’ll need about 500 mL to 1 L of culture media, like DMEM or RPMI 1640, for each culture. Keeping things sterile is important, so using a Laminar Flow Hood is recommended. But if you don’t have one, setting up a clean workspace in a well-ventilated room can work too. The process is pretty simple: just pour the media into a flask, then add some Fetal Bovine Serum (FBS) and antibiotics.
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- FBS: Add 5-20% of the total media volume.
- Antibiotics (Penicillin/Streptomycin): Add 1% of the media volume to keep bacteria from messing things up.
It’s taking me too long to find a source for everything. I’m sure you can find a source somewhere for these ingredients.
Growth Factors and Chemicals
SAG (Smoothened Agonist): This stuff kicks off pathways that help cells grow. You’ll want to use a concentration of 1-10 µM. If you want to see how it’s working, you can use a Microplate Reader, but if you’re on a budget, a regular spectrophotometer works too. Just dilute the SAG and add it to your cell cultures.
IGF-1 (Recombinant Human IGF-1): This promotes cell growth and keeps them alive. You’ll need to add 10-100 ng/mL to your culture media.
Tamoxifen: This controls gene expression by hooking up with estrogen receptors. Use a concentration of 1-10 µM for this one.
SAG 1 mg | Buy Online | Thermo Scientific™ | thermofisher.com
SAG. SAG. Available in 1 mgwww.thermofisher.com
Tamoxifen, 98% 500 mg | Buy Online | Thermo Scientific Chemicals | thermofisher.com
Tamoxifen, 98%. Tamoxifen, 10540-29-1, is an antineoplastic agent that acts by competitively binding estrogen receptors to reduce DNA synthesis. Learn more at Thermo Fisher Scientific. Available in 500 mgwww.thermofisher.com
Verification and Analysis
PCR (Polymerase Chain Reaction): PCR is a technique used to make a bunch of copies of a specific DNA segment so you can analyze it. To do this, you'll need a concentration of about 0.1-1 µM and a machine called a Thermal Cycler
Sequencing (Sanger or Next-Generation): Sequencing is the method used to figure out the exact order of DNA bases, which helps you confirm any changes or edits you made. The amount of DNA you’ll need depends on which sequencing service you’re using.
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1. Getting the Solution Ready
First off, you need to mix up the solution:
- Sterilization: Make sure all your gear (like syringes, needles, and vials) is clean and sterilized to prevent contamination.
- Mixing It Up: Combine the SAG, IGF-1, and Tamoxifen in a clean vial. Give it a gentle swirl to mix everything—don’t shake it too hard, or you might mess up the mixture!
2. Filling the Syringe
Next, you’ve gotta get the solution into the syringe:
- Draw It Up: Attach the needle to the syringe and carefully draw the solution into it. Be sure to avoid any air bubbles.
- Check the Amount: Double-check that you’ve got the right amount in the syringe. Accurate dosing is crucial.
- Example Dosages: If you’re injecting 100 µL per site, ensure your mix has the correct amount of each ingredient.
3. Prepping the Injection Spot
Now, let’s get the injection site ready:
View attachment 3134721
- Keep It Clean: Perform this step in a very clean environment to avoid any contamination.
- Find the Secondary Ossification Center: While it’s best to use imaging tools like X-rays, if that’s not an option, you can locate the general area based on anatomical landmarks. The secondary ossification center is typically located near the ends of long bones, such as the femur, close to the joint. You might feel for the softer areas at the ends of bones where growth plates are located.
- Mark It: Once you’ve identified the area near the joint where the secondary ossification center is likely located, mark the spot carefully to ensure accurate injection.
4. Doing the Injection
Here’s how you do the injection:
- Clean the Area: Wipe down the marked spot with antiseptic to keep it clean.
- Stick the Needle In: Carefully insert the needle into the marked spot, aiming for the softer, slightly indented area near the joint, which is indicative of the secondary ossification center.
- Inject the Solution: Slowly inject the solution into the area. Do it steadily to ensure it goes in smoothly.
- How Much to Use: Typically, 100 µL per secondary ossification center should be sufficient, as suggested by the study. (Since you're a human and not a rat, the dosages might not be sufficient. This is all hypothetical, though, lol)
Well, this was fun to make. I doubt anyone will actually do this it's all hypothetical and costs a shit ton of money. Plus, it hasn't been tested in human trials yet, so the dosages might be off depending on your weight and other factors. I'm just going by the study, but yeah. I probably messed up a few times because I'm not a scientist, but let me know your thoughts do you think this would work on humans?
