How to properly use GHK-Cu

[russiancelreturns]

BAC stomach,almost no pip at all

this is how ive been doing it for a year but see no difference with the exception of slight eyelashes growth, i consider switching to pbs

 

[iblamewill]

Pbs is only good for 24 hours no? What do I do about the 50mg vials when I only want 2.5 throw out the rest

 

[UrFavNarcis]

it does

View attachment 3279753

I got a question if ghk kills igf1 should i take it while being on hgh cuz hgh boosts igf1 after all? Or do I stop it while i cycle hgh

 

[Valerium]

I've used ghk-cu for around 2 months now and I've noticed a huge different in how my skin looks. It used to look very "dead" now it shines like crazy, I might consider taking mt-2 or mt-1 because I do need a tan. It is very hard to tan where I live since the sun almost never shows over here in Sweden.

 

[foidslayer8888]

Bookmarked

 

[BreezyVII]

Yes you injected GHK,, which still boosts collagen

View attachment 3279082,

but not GHK-Cu or in insufficient amounts (most of it was busy causing the bruising on the injection site)

Most of the benefits though are from GHK-Cu yes

How do you store this tho? Won’t it go bad after like a day as it has no preservatives, you might of said something and I missed it or I’m just wrong idk

GHK-Cu, on paper, seems to be the single best looksmaxxing compound there is (perfect skin, anti-aging, surgery recovery, neurogenesis⁹,¹¹ etc)¹, yet no one seems to be reaping any of its benefits. What’s even more bizarre is that injection-related pain is massively reported, more consistently so than its supposed benefits².

Quickly, Glycyl-L-histidyl-L-lysine (GHK) is a tripeptide isolated from human plasma³, which possesses a high affinity for copper (II) ions, with which it spontaneously forms a complex (GHK-Cu). GHK alone has been shown to be responsible for the collagen boost⁴ and no injection-related pain has been reported with it⁵, so go for it if you want but it's the added copper that is responsible for this myriad of blessings;

EXAMPLES :
The complex, unlike GHK alone, helps activate matrix metalloproteinases, which break down damaged tissue so it can be replaced with healthy tissue⁶.

View attachment 3275853

OR

Compositions of copper (II) containing compounds have been show to accelerate the healing of wounds in warm-blooded animals, and more specifically the systemic loading of copper (II) compounds to accelerate the rate of wound healing following injury or surgery to warm-blooded animals.⁷

View attachment 3275859


; ...and consequently what seems to cause tissue damage during injection.


If we look at the cayman chemicals reviews of the molecules in question :


View attachment 3275820View attachment 3275821


The main differences are the addition of a copper atom and a significantly worse solubility profile, which suggests a causal relationship between these two differences (meaning that the added copper induces the worse solubility).

In fact, we know that metals are gay

Spoiler: Gay

Gay meaning they have different bonding properties vs carbon, oxygen, etc (this whole field of gay metals is “inorganic chemistry”) The reason for this is that metals basically have up to ten relevant electrons , which have comically complex geometries, are much larger (radius) and less bound to the nucelus

View attachment 3275870

Simply, they like to move a lot, they're playful building blocks that like to change and mix , while other atoms are stronger and stick together better, other atoms, like those in water or air, are like super strong blocks that hold together really well. They don’t like to change as much, so they are usually more stable.




Seriously, metals require ions to stabilize in various contexts, primarily due to their tendency to lose electrons and form positive charges, the ions in PBS can neutralize the positive charge of the copper ions, reducing the likelihood of oxidation or other reactions that would destabilize the copper. PBS also maintains a stable pH, which is important for keeping copper in its soluble form. In bac water or regular saline, changes in pH can lead to precipitation or oxidation of copper ions. (5 to 7 for bac and reg saline vs 7.2 to 7.4 for Phosphate Buffered Saline)

Hence why GHK-Cu needs PBS (again because the ionic solution (has a lot of ions) stabilizes the added copper), otherwise the copper-nitrogen bonds are likely to break.
View attachment 3276079


The common folk seems to be dissolving GHK-Cu in Bac Water since that is the usual commercially available and promoted solvent for peptides².
However, Bac Water has a lower ion concentration than PBS (which makes the copper less likely to be stable), I hypothesize that the pain comes from the free copper atoms raping your tissues (not fully understood).

View attachment 3275864

Injection solutions :

Phosphate Buffered Saline :

https://sci-hub.ru/10.1172/JCI116842

View attachment 3275998

https://onlinelibrary.wiley.com/doi/epdf/10.1002/jor.22831

View attachment 3276003

https://sci-hub.ru/10.1046/j.1523-1747.1999.00606.x

View attachment 3276026


i.e. what is mostly used in studies and what is recommended by CaymanChem

Making PBS at home looks annoying af so just fucking buy it : https://www.sigmaaldrich.com/US/en/product/sigma/d8537

WORKS AND STABLE



Normale saline (0.9% saline) :

https://d1wqtxts1xzle7.cloudfront.net/37163377/Massey_Patt_Aoust_The_Effect_of_GHK-Cu_on_the_Healing_of_Diabet_Ulcers_A_Pilot_Sutyd_WOUNDS_4_21-28_1992-libre.PDF?1427753974=&response-content-disposition=attachment%3B+filename%3DMassey_Patt_Aoust_The_Effect_of_GHK_Cu_o.pdf&Expires=1730648621&Signature=bEi6x2QXzs~nLvVNqdEnYmv7~ruaYJ3wGxGnHPUBnbcUmupFy9OMJu7w2M8FOsNd6ksfpzr4TNj-7184G5MT1rk224QmlJ37S6yYnjzsLTWhqjIZqtcp~Lsf6Qy~xxDEnhMbmh~DjIE891q1xoUH4l6V73w5kDgB3C-e~V06E1gnd9vJi0sdDLj5Eo0gGSVBWxV3fF2IF7cfHo9l36YXHJgRoFpOyyYKZjlF3rN-dWJ6C7VRRq8iAz8YgtCExeNyzDJA0l0HYIjE5xS65171U10rPPBRcZ5aOFzhieuFk7awwfSbKg5COwUvLmUzf9RyiTSZKci8BhSDDSbQYj6h-A__&Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA

Direct on-site injection tho

View attachment 3276031


View attachment 3276018View attachment 3276019

WORKS BUT THEORETICALLY NOT STABLE, since the PH will be too low

You'd need to adjsut it yourself⁷

View attachment 3276064

BAC WATER :

DOESN'T WORK (YOU ONLY GET GHK AND INJECTION SITE DAMAGE)²




Topical :

Topical formulations of GHKcu have been developed and are effective

BetTar:

The best one (both low-cost, most effective and most stable) being Bet Tar which is a recent (2023) chinese developped ionic-liquid-based delivery vehicle composed of betaine and tartaric acid

View attachment 3276085

View attachment 3276086
View attachment 3276090

Stable as fuck :
View attachment 3276087

PDI is dispersity

I will be attaching the papers⁸ in case President Xi takes them down

Hyaluronic acid-based hydrogel embedded with GHK peptide nanofibers : ( )

Works af for direct wound healing

In situ photo-crosslinkable hyaluronic acid-based hydrogel embedded with GHK peptide nanofibers for bioactive wound healing

A versatile hydrogel was developed for enhancing bioactive wound healing by introducing the amphiphilic GHK peptide (GHK-C16) into a photo-crosslinkab…

www.sciencedirect.com

View attachment 3276113

YES BRO I WILL TOTALLY MAKE THIS jfl

View attachment 3276114


THERE ARE 8 BILLION GOOD TOPICAL ways but WE CAN NOT MAKE/BUY any of these

This was good for wound closure but go figure how they made it

https://sci-hub.ru/10.1046/j.1524-475X.1994.20406.x

View attachment 3276058View attachment 3276117

Concering DMSO :

IT DOESN'T WORK (IT'S EVEN LESS STABLE THAN BAC WATER since it has less ions (water (bac water) has H+ and OH- (water)))

These niggas tried to study its structure while dissolving it in DMSO¹⁰ : https://sci-hub.ru/10.1016/0162-0134(89)84011-5, which gave them completly retarded results "under their conditions"

Metals in general seem to be struggling in DMSO
View attachment 3276159

Goodluck rabbitholemaxxing this

https://sci-hub.ru/10.1016/j.ccr.2004.02.005

TLDR :



- RECONSTITUTE GHK-Cu IN PBS or DPBS (which you can buy) and inject it daily

- BUY and RECONSTITUTE GHK IN BAC WATER IF YOU ONLY WANT COLLAGEN BOOST AND EASE OF USE and inject it daily

- TRY TO MAKE a [Bet] [Tar] GHK-Cu Topical Liquid Solution, if you want, for when you can't inject or as first-aid

Spoiler: Tags

@Hexmask @N1666 @Clavicular @NorwoodAscender @meathead

¹Pickart, L., & Margolina, A. (2018). Regenerative and protective actions of the GHK-Cu peptide in the light of the new gene data. International Journal of Molecular Sciences, 19(7), https://pmc.ncbi.nlm.nih.gov/articles/PMC6073405/pdf/ijms-19-01987.pdf
²
³Pickart L, Thaler MM. Tripeptide in human serum which prolongs survival of normal liver cells and stimulates growth in neoplastic liver. Nat New Biol. 1973 May 16;243(124):85-7. PMID: 4349963.
⁴Maquart, F.-X., Pickart, L., Laurent, M., Gillery, P., Monboisse, J.-C., & Borel, J.-P. (1988). Stimulation of collagen synthesis in fibroblast cultures by the tripeptide-copper complex glycyl-L-histidyl-L-lysine-Cu²⁺. Biochemical and Biophysical Research Communications, 238(2), 343-346. https://sci-hub.ru/10.1016/0014-5793(88)80509-X
⁵ Google it and add Reddit and various forums to the key-words
⁶Siméon, A., Emonard, H., Hornebeck, W., & Maquart, F.-X. (2000). The tripeptide-copper complex glycyl-L-histidyl-L-lysine-Cu²⁺ stimulates matrix metalloproteinase-2 expression by fibroblast cultures. Life Sciences, 67(20), 2257-2265. https://sci-hub.ru/10.1016/S0024-3205(00)00803-1
⁷ Pickart, L. R. (1992). Method of using copper(II) containing compounds to accelerate wound healing (U.S. Patent No. 5164367). U.S. Patent and Trademark Office. https://patentimages.storage.googleapis.com/28/c1/c7/6fb3bbcf61becb/US5164367.pdf
⁸Liu, T., Hu, L., Lu, B., Bo, Y., Liao, Y., Zhan, J., Pei, Y., Sun, H., Wang, Z., Guo, C., & Zhang, J. (2023). A novel delivery vehicle for copper peptides. New Journal of Chemistry https://pubs.rsc.org/en/content/articlelanding/2023/nj/d2nj04282g#fn1
⁹L. Pickart, in BioIogy of Copper Complexes, J. R. C. Sorenson, Ed., Human Press, Clifton, NJ, 1987, pp. 273-285 (https://library.lol/main/04A4B0236383B58B30E1AF569542355F), neurogenesis also mentionned here¹⁰
¹⁰Antholine, W. E., Petering, D. H., & Pickart, L. (1989). ESR studies of the interaction of copper(II) GHK, histidine, and Ehrlich cells. Journal of Inorganic Biochemistry, 35(2), U-224
¹¹Pickart, L., Vasquez-Soltero, J. M., & Margolina, A. (2012). The human tripeptide GHK-Cu in prevention of oxidative stress and degenerative conditions of aging: Implications for cognitive health. Oxidative Medicine and Cellular Longevity, 2012, Article ID 324832. https://doi.org/10.1155/2012/324832

 

[plzgodhelpme]

Topical :

Topical formulations of GHKcu have been developed and are effective

BetTar:

The best one (both low-cost, most effective and most stable) being Bet Tar which is a recent (2023) chinese developped ionic-liquid-based delivery vehicle composed of betaine and tartaric acid

View attachment 3276085

View attachment 3276086
View attachment 3276090

Stable as fuck :
View attachment 3276087

where can i find such topical soultions ?

 

[russocel]

GHK-Cu, on paper, seems to be the single best looksmaxxing compound there is (perfect skin, anti-aging, surgery recovery, neurogenesis⁹,¹¹ etc)¹, yet no one seems to be reaping any of its benefits. What’s even more bizarre is that injection-related pain is massively reported, more consistently so than its supposed benefits².

Quickly, Glycyl-L-histidyl-L-lysine (GHK) is a tripeptide isolated from human plasma³, which possesses a high affinity for copper (II) ions, with which it spontaneously forms a complex (GHK-Cu). GHK alone has been shown to be responsible for the collagen boost⁴ and no injection-related pain has been reported with it⁵, so go for it if you want but it's the added copper that is responsible for this myriad of blessings;

EXAMPLES :
The complex, unlike GHK alone, helps activate matrix metalloproteinases, which break down damaged tissue so it can be replaced with healthy tissue⁶.

View attachment 3275853

OR

Compositions of copper (II) containing compounds have been show to accelerate the healing of wounds in warm-blooded animals, and more specifically the systemic loading of copper (II) compounds to accelerate the rate of wound healing following injury or surgery to warm-blooded animals.⁷

View attachment 3275859


; ...and consequently what seems to cause tissue damage during injection.


If we look at the cayman chemicals reviews of the molecules in question :


View attachment 3275820View attachment 3275821


The main differences are the addition of a copper atom and a significantly worse solubility profile, which suggests a causal relationship between these two differences (meaning that the added copper induces the worse solubility).

In fact, we know that metals are gay

Spoiler: Gay

Gay meaning they have different bonding properties vs carbon, oxygen, etc (this whole field of gay metals is “inorganic chemistry”) The reason for this is that metals basically have up to ten relevant electrons , which have comically complex geometries, are much larger (radius) and less bound to the nucelus

View attachment 3275870

Simply, they like to move a lot, they're playful building blocks that like to change and mix , while other atoms are stronger and stick together better, other atoms, like those in water or air, are like super strong blocks that hold together really well. They don’t like to change as much, so they are usually more stable.




Seriously, metals require ions to stabilize in various contexts, primarily due to their tendency to lose electrons and form positive charges, the ions in PBS can neutralize the positive charge of the copper ions, reducing the likelihood of oxidation or other reactions that would destabilize the copper. PBS also maintains a stable pH, which is important for keeping copper in its soluble form. In bac water or regular saline, changes in pH can lead to precipitation or oxidation of copper ions. (5 to 7 for bac and reg saline vs 7.2 to 7.4 for Phosphate Buffered Saline)

Hence why GHK-Cu needs PBS (again because the ionic solution (has a lot of ions) stabilizes the added copper), otherwise the copper-nitrogen bonds are likely to break.
View attachment 3276079


The common folk seems to be dissolving GHK-Cu in Bac Water since that is the usual commercially available and promoted solvent for peptides².
However, Bac Water has a lower ion concentration than PBS (which makes the copper less likely to be stable), I hypothesize that the pain comes from the free copper atoms raping your tissues (not fully understood).

View attachment 3275864

Injection solutions :

Phosphate Buffered Saline :

https://sci-hub.ru/10.1172/JCI116842

View attachment 3275998

https://onlinelibrary.wiley.com/doi/epdf/10.1002/jor.22831

View attachment 3276003

https://sci-hub.ru/10.1046/j.1523-1747.1999.00606.x

View attachment 3276026


i.e. what is mostly used in studies and what is recommended by CaymanChem

Making PBS at home looks annoying af so just fucking buy it : https://www.sigmaaldrich.com/US/en/product/sigma/d8537

WORKS AND STABLE



Normale saline (0.9% saline) :

https://d1wqtxts1xzle7.cloudfront.net/37163377/Massey_Patt_Aoust_The_Effect_of_GHK-Cu_on_the_Healing_of_Diabet_Ulcers_A_Pilot_Sutyd_WOUNDS_4_21-28_1992-libre.PDF?1427753974=&response-content-disposition=attachment%3B+filename%3DMassey_Patt_Aoust_The_Effect_of_GHK_Cu_o.pdf&Expires=1730648621&Signature=bEi6x2QXzs~nLvVNqdEnYmv7~ruaYJ3wGxGnHPUBnbcUmupFy9OMJu7w2M8FOsNd6ksfpzr4TNj-7184G5MT1rk224QmlJ37S6yYnjzsLTWhqjIZqtcp~Lsf6Qy~xxDEnhMbmh~DjIE891q1xoUH4l6V73w5kDgB3C-e~V06E1gnd9vJi0sdDLj5Eo0gGSVBWxV3fF2IF7cfHo9l36YXHJgRoFpOyyYKZjlF3rN-dWJ6C7VRRq8iAz8YgtCExeNyzDJA0l0HYIjE5xS65171U10rPPBRcZ5aOFzhieuFk7awwfSbKg5COwUvLmUzf9RyiTSZKci8BhSDDSbQYj6h-A__&Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA

Direct on-site injection tho

View attachment 3276031


View attachment 3276018View attachment 3276019

WORKS BUT THEORETICALLY NOT STABLE, since the PH will be too low

You'd need to adjsut it yourself⁷

View attachment 3276064

BAC WATER :

DOESN'T WORK (YOU ONLY GET GHK AND INJECTION SITE DAMAGE)²




Topical :

Topical formulations of GHKcu have been developed and are effective

BetTar:

The best one (both low-cost, most effective and most stable) being Bet Tar which is a recent (2023) chinese developped ionic-liquid-based delivery vehicle composed of betaine and tartaric acid

View attachment 3276085

View attachment 3276086
View attachment 3276090

Stable as fuck :
View attachment 3276087

PDI is dispersity

I will be attaching the papers⁸ in case President Xi takes them down

Hyaluronic acid-based hydrogel embedded with GHK peptide nanofibers : ( )

Works af for direct wound healing

In situ photo-crosslinkable hyaluronic acid-based hydrogel embedded with GHK peptide nanofibers for bioactive wound healing

A versatile hydrogel was developed for enhancing bioactive wound healing by introducing the amphiphilic GHK peptide (GHK-C16) into a photo-crosslinkab…

www.sciencedirect.com

View attachment 3276113

YES BRO I WILL TOTALLY MAKE THIS jfl

View attachment 3276114


THERE ARE 8 BILLION GOOD TOPICAL ways but WE CAN NOT MAKE/BUY any of these

This was good for wound closure but go figure how they made it

https://sci-hub.ru/10.1046/j.1524-475X.1994.20406.x

View attachment 3276058View attachment 3276117

Concering DMSO :

IT DOESN'T WORK (IT'S EVEN LESS STABLE THAN BAC WATER since it has less ions (water (bac water) has H+ and OH- (water)))

These niggas tried to study its structure while dissolving it in DMSO¹⁰ : https://sci-hub.ru/10.1016/0162-0134(89)84011-5, which gave them completly retarded results "under their conditions"

Metals in general seem to be struggling in DMSO
View attachment 3276159

Goodluck rabbitholemaxxing this

https://sci-hub.ru/10.1016/j.ccr.2004.02.005

TLDR :



- RECONSTITUTE GHK-Cu IN PBS or DPBS (which you can buy) and inject it daily

- BUY and RECONSTITUTE GHK IN BAC WATER IF YOU ONLY WANT COLLAGEN BOOST AND EASE OF USE and inject it daily

- TRY TO MAKE a [Bet] [Tar] GHK-Cu Topical Liquid Solution, if you want, for when you can't inject or as first-aid

Spoiler: Tags

@Hexmask @N1666 @Clavicular @NorwoodAscender @meathead

¹Pickart, L., & Margolina, A. (2018). Regenerative and protective actions of the GHK-Cu peptide in the light of the new gene data. International Journal of Molecular Sciences, 19(7), https://pmc.ncbi.nlm.nih.gov/articles/PMC6073405/pdf/ijms-19-01987.pdf
²
³Pickart L, Thaler MM. Tripeptide in human serum which prolongs survival of normal liver cells and stimulates growth in neoplastic liver. Nat New Biol. 1973 May 16;243(124):85-7. PMID: 4349963.
⁴Maquart, F.-X., Pickart, L., Laurent, M., Gillery, P., Monboisse, J.-C., & Borel, J.-P. (1988). Stimulation of collagen synthesis in fibroblast cultures by the tripeptide-copper complex glycyl-L-histidyl-L-lysine-Cu²⁺. Biochemical and Biophysical Research Communications, 238(2), 343-346. https://sci-hub.ru/10.1016/0014-5793(88)80509-X
⁵ Google it and add Reddit and various forums to the key-words
⁶Siméon, A., Emonard, H., Hornebeck, W., & Maquart, F.-X. (2000). The tripeptide-copper complex glycyl-L-histidyl-L-lysine-Cu²⁺ stimulates matrix metalloproteinase-2 expression by fibroblast cultures. Life Sciences, 67(20), 2257-2265. https://sci-hub.ru/10.1016/S0024-3205(00)00803-1
⁷ Pickart, L. R. (1992). Method of using copper(II) containing compounds to accelerate wound healing (U.S. Patent No. 5164367). U.S. Patent and Trademark Office. https://patentimages.storage.googleapis.com/28/c1/c7/6fb3bbcf61becb/US5164367.pdf
⁸Liu, T., Hu, L., Lu, B., Bo, Y., Liao, Y., Zhan, J., Pei, Y., Sun, H., Wang, Z., Guo, C., & Zhang, J. (2023). A novel delivery vehicle for copper peptides. New Journal of Chemistry https://pubs.rsc.org/en/content/articlelanding/2023/nj/d2nj04282g#fn1
⁹L. Pickart, in BioIogy of Copper Complexes, J. R. C. Sorenson, Ed., Human Press, Clifton, NJ, 1987, pp. 273-285 (https://library.lol/main/04A4B0236383B58B30E1AF569542355F), neurogenesis also mentionned here¹⁰
¹⁰Antholine, W. E., Petering, D. H., & Pickart, L. (1989). ESR studies of the interaction of copper(II) GHK, histidine, and Ehrlich cells. Journal of Inorganic Biochemistry, 35(2), U-224
¹¹Pickart, L., Vasquez-Soltero, J. M., & Margolina, A. (2012). The human tripeptide GHK-Cu in prevention of oxidative stress and degenerative conditions of aging: Implications for cognitive health. Oxidative Medicine and Cellular Longevity, 2012, Article ID 324832. https://doi.org/10.1155/2012/324832

appreciate the post, hopping on ghk tomorrow for 3 months.

will update if you ask.

 

[russocel]

where can i find such topical soultions ?

Try not to use GHK-CU topically. If you're afraid of possible side effects, just know that they are minimal with the only noticeable systemic side effect being the possibility of sensitive skin. Lot's of topical GHK supplements don't even contain GHK ironically, but only accelerants which essentially point your body towards the same effects of GHK. Pinning GHK is not that hard and boasts immense benefits, hence why I recommend it.

If you need any questions regarding GHK just let me know and I'll answer to the best of my abilities.

 

[russocel]

How do you store this tho? Won’t it go bad after like a day as it has no preservatives, you might of said something and I missed it or I’m just wrong idk

To store GHK-CU it's best to only use one 50mg vial at a time after adding your bacteriostatic water because the shelf-life in GHK when stored in your typical refrigerator (2C - 8C) is about a month before quality starts to diminish.

 

[Kojo]

GHK-Cu, on paper, seems to be the single best looksmaxxing compound there is (perfect skin, anti-aging, surgery recovery, neurogenesis⁹,¹¹ etc)¹, yet no one seems to be reaping any of its benefits. What’s even more bizarre is that injection-related pain is massively reported, more consistently so than its supposed benefits².

Quickly, Glycyl-L-histidyl-L-lysine (GHK) is a tripeptide isolated from human plasma³, which possesses a high affinity for copper (II) ions, with which it spontaneously forms a complex (GHK-Cu). GHK alone has been shown to be responsible for the collagen boost⁴ and no injection-related pain has been reported with it⁵, so go for it if you want but it's the added copper that is responsible for this myriad of blessings;

EXAMPLES :
The complex, unlike GHK alone, helps activate matrix metalloproteinases, which break down damaged tissue so it can be replaced with healthy tissue⁶.

View attachment 3275853

OR

Compositions of copper (II) containing compounds have been show to accelerate the healing of wounds in warm-blooded animals, and more specifically the systemic loading of copper (II) compounds to accelerate the rate of wound healing following injury or surgery to warm-blooded animals.⁷

View attachment 3275859


; ...and consequently what seems to cause tissue damage during injection.


If we look at the cayman chemicals reviews of the molecules in question :


View attachment 3275820View attachment 3275821


The main differences are the addition of a copper atom and a significantly worse solubility profile, which suggests a causal relationship between these two differences (meaning that the added copper induces the worse solubility).

In fact, we know that metals are gay

Spoiler: Gay

Gay meaning they have different bonding properties vs carbon, oxygen, etc (this whole field of gay metals is “inorganic chemistry”) The reason for this is that metals basically have up to ten relevant electrons , which have comically complex geometries, are much larger (radius) and less bound to the nucelus

View attachment 3275870

Simply, they like to move a lot, they're playful building blocks that like to change and mix , while other atoms are stronger and stick together better, other atoms, like those in water or air, are like super strong blocks that hold together really well. They don’t like to change as much, so they are usually more stable.




Seriously, metals require ions to stabilize in various contexts, primarily due to their tendency to lose electrons and form positive charges, the ions in PBS can neutralize the positive charge of the copper ions, reducing the likelihood of oxidation or other reactions that would destabilize the copper. PBS also maintains a stable pH, which is important for keeping copper in its soluble form. In bac water or regular saline, changes in pH can lead to precipitation or oxidation of copper ions. (5 to 7 for bac and reg saline vs 7.2 to 7.4 for Phosphate Buffered Saline)

Hence why GHK-Cu needs PBS (again because the ionic solution (has a lot of ions) stabilizes the added copper), otherwise the copper-nitrogen bonds are likely to break.
View attachment 3276079


The common folk seems to be dissolving GHK-Cu in Bac Water since that is the usual commercially available and promoted solvent for peptides².
However, Bac Water has a lower ion concentration than PBS (which makes the copper less likely to be stable), I hypothesize that the pain comes from the free copper atoms raping your tissues (not fully understood).

View attachment 3275864

Injection solutions :

Phosphate Buffered Saline :

https://sci-hub.ru/10.1172/JCI116842

View attachment 3275998

https://onlinelibrary.wiley.com/doi/epdf/10.1002/jor.22831

View attachment 3276003

https://sci-hub.ru/10.1046/j.1523-1747.1999.00606.x

View attachment 3276026


i.e. what is mostly used in studies and what is recommended by CaymanChem

Making PBS at home looks annoying af so just fucking buy it : https://www.sigmaaldrich.com/US/en/product/sigma/d8537

WORKS AND STABLE



Normale saline (0.9% saline) :

https://d1wqtxts1xzle7.cloudfront.net/37163377/Massey_Patt_Aoust_The_Effect_of_GHK-Cu_on_the_Healing_of_Diabet_Ulcers_A_Pilot_Sutyd_WOUNDS_4_21-28_1992-libre.PDF?1427753974=&response-content-disposition=attachment%3B+filename%3DMassey_Patt_Aoust_The_Effect_of_GHK_Cu_o.pdf&Expires=1730648621&Signature=bEi6x2QXzs~nLvVNqdEnYmv7~ruaYJ3wGxGnHPUBnbcUmupFy9OMJu7w2M8FOsNd6ksfpzr4TNj-7184G5MT1rk224QmlJ37S6yYnjzsLTWhqjIZqtcp~Lsf6Qy~xxDEnhMbmh~DjIE891q1xoUH4l6V73w5kDgB3C-e~V06E1gnd9vJi0sdDLj5Eo0gGSVBWxV3fF2IF7cfHo9l36YXHJgRoFpOyyYKZjlF3rN-dWJ6C7VRRq8iAz8YgtCExeNyzDJA0l0HYIjE5xS65171U10rPPBRcZ5aOFzhieuFk7awwfSbKg5COwUvLmUzf9RyiTSZKci8BhSDDSbQYj6h-A__&Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA

Direct on-site injection tho

View attachment 3276031


View attachment 3276018View attachment 3276019

WORKS BUT THEORETICALLY NOT STABLE, since the PH will be too low

You'd need to adjsut it yourself⁷

View attachment 3276064

BAC WATER :

DOESN'T WORK (YOU ONLY GET GHK AND INJECTION SITE DAMAGE)²




Topical :

Topical formulations of GHKcu have been developed and are effective

BetTar:

The best one (both low-cost, most effective and most stable) being Bet Tar which is a recent (2023) chinese developped ionic-liquid-based delivery vehicle composed of betaine and tartaric acid

View attachment 3276085

View attachment 3276086
View attachment 3276090

Stable as fuck :
View attachment 3276087

PDI is dispersity

I will be attaching the papers⁸ in case President Xi takes them down

Hyaluronic acid-based hydrogel embedded with GHK peptide nanofibers : ( )

Works af for direct wound healing

In situ photo-crosslinkable hyaluronic acid-based hydrogel embedded with GHK peptide nanofibers for bioactive wound healing

A versatile hydrogel was developed for enhancing bioactive wound healing by introducing the amphiphilic GHK peptide (GHK-C16) into a photo-crosslinkab…

www.sciencedirect.com

View attachment 3276113

YES BRO I WILL TOTALLY MAKE THIS jfl

View attachment 3276114


THERE ARE 8 BILLION GOOD TOPICAL ways but WE CAN NOT MAKE/BUY any of these

This was good for wound closure but go figure how they made it

https://sci-hub.ru/10.1046/j.1524-475X.1994.20406.x

View attachment 3276058View attachment 3276117

Concering DMSO :

IT DOESN'T WORK (IT'S EVEN LESS STABLE THAN BAC WATER since it has less ions (water (bac water) has H+ and OH- (water)))

These niggas tried to study its structure while dissolving it in DMSO¹⁰ : https://sci-hub.ru/10.1016/0162-0134(89)84011-5, which gave them completly retarded results "under their conditions"

Metals in general seem to be struggling in DMSO
View attachment 3276159

Goodluck rabbitholemaxxing this

https://sci-hub.ru/10.1016/j.ccr.2004.02.005

TLDR :



- RECONSTITUTE GHK-Cu IN PBS or DPBS (which you can buy) and inject it daily

- BUY and RECONSTITUTE GHK IN BAC WATER IF YOU ONLY WANT COLLAGEN BOOST AND EASE OF USE and inject it daily

- TRY TO MAKE a [Bet] [Tar] GHK-Cu Topical Liquid Solution, if you want, for when you can't inject or as first-aid

Spoiler: Tags

@Hexmask @N1666 @Clavicular @NorwoodAscender @meathead

¹Pickart, L., & Margolina, A. (2018). Regenerative and protective actions of the GHK-Cu peptide in the light of the new gene data. International Journal of Molecular Sciences, 19(7), https://pmc.ncbi.nlm.nih.gov/articles/PMC6073405/pdf/ijms-19-01987.pdf
²
³Pickart L, Thaler MM. Tripeptide in human serum which prolongs survival of normal liver cells and stimulates growth in neoplastic liver. Nat New Biol. 1973 May 16;243(124):85-7. PMID: 4349963.
⁴Maquart, F.-X., Pickart, L., Laurent, M., Gillery, P., Monboisse, J.-C., & Borel, J.-P. (1988). Stimulation of collagen synthesis in fibroblast cultures by the tripeptide-copper complex glycyl-L-histidyl-L-lysine-Cu²⁺. Biochemical and Biophysical Research Communications, 238(2), 343-346. https://sci-hub.ru/10.1016/0014-5793(88)80509-X
⁵ Google it and add Reddit and various forums to the key-words
⁶Siméon, A., Emonard, H., Hornebeck, W., & Maquart, F.-X. (2000). The tripeptide-copper complex glycyl-L-histidyl-L-lysine-Cu²⁺ stimulates matrix metalloproteinase-2 expression by fibroblast cultures. Life Sciences, 67(20), 2257-2265. https://sci-hub.ru/10.1016/S0024-3205(00)00803-1
⁷ Pickart, L. R. (1992). Method of using copper(II) containing compounds to accelerate wound healing (U.S. Patent No. 5164367). U.S. Patent and Trademark Office. https://patentimages.storage.googleapis.com/28/c1/c7/6fb3bbcf61becb/US5164367.pdf
⁸Liu, T., Hu, L., Lu, B., Bo, Y., Liao, Y., Zhan, J., Pei, Y., Sun, H., Wang, Z., Guo, C., & Zhang, J. (2023). A novel delivery vehicle for copper peptides. New Journal of Chemistry https://pubs.rsc.org/en/content/articlelanding/2023/nj/d2nj04282g#fn1
⁹L. Pickart, in BioIogy of Copper Complexes, J. R. C. Sorenson, Ed., Human Press, Clifton, NJ, 1987, pp. 273-285 (https://library.lol/main/04A4B0236383B58B30E1AF569542355F), neurogenesis also mentionned here¹⁰
¹⁰Antholine, W. E., Petering, D. H., & Pickart, L. (1989). ESR studies of the interaction of copper(II) GHK, histidine, and Ehrlich cells. Journal of Inorganic Biochemistry, 35(2), U-224
¹¹Pickart, L., Vasquez-Soltero, J. M., & Margolina, A. (2012). The human tripeptide GHK-Cu in prevention of oxidative stress and degenerative conditions of aging: Implications for cognitive health. Oxidative Medicine and Cellular Longevity, 2012, Article ID 324832. https://doi.org/10.1155/2012/324832

Not a molecule

 

[acsended]

Whats your source? I am looking for one in EU that is lab tested...

get some chinese one

 

[pslgod15]

ghk-cu topic the best

 

[acsended]

dnr

 

[JGoat]

GHK-Cu, on paper, seems to be the single best looksmaxxing compound there is (perfect skin, anti-aging, surgery recovery, neurogenesis⁹,¹¹ etc)¹, yet no one seems to be reaping any of its benefits. What’s even more bizarre is that injection-related pain is massively reported, more consistently so than its supposed benefits².

Quickly, Glycyl-L-histidyl-L-lysine (GHK) is a tripeptide isolated from human plasma³, which possesses a high affinity for copper (II) ions, with which it spontaneously forms a complex (GHK-Cu). GHK alone has been shown to be responsible for the collagen boost⁴ and no injection-related pain has been reported with it⁵, so go for it if you want but it's the added copper that is responsible for this myriad of blessings;

EXAMPLES :
The complex, unlike GHK alone, helps activate matrix metalloproteinases, which break down damaged tissue so it can be replaced with healthy tissue⁶.

View attachment 3275853

OR

Compositions of copper (II) containing compounds have been show to accelerate the healing of wounds in warm-blooded animals, and more specifically the systemic loading of copper (II) compounds to accelerate the rate of wound healing following injury or surgery to warm-blooded animals.⁷

View attachment 3275859


; ...and consequently what seems to cause tissue damage during injection.


If we look at the cayman chemicals reviews of the molecules in question :


View attachment 3275820View attachment 3275821


The main differences are the addition of a copper atom and a significantly worse solubility profile, which suggests a causal relationship between these two differences (meaning that the added copper induces the worse solubility).

In fact, we know that metals are gay

Spoiler: Gay

Gay meaning they have different bonding properties vs carbon, oxygen, etc (this whole field of gay metals is “inorganic chemistry”) The reason for this is that metals basically have up to ten relevant electrons , which have comically complex geometries, are much larger (radius) and less bound to the nucelus

View attachment 3275870

Simply, they like to move a lot, they're playful building blocks that like to change and mix , while other atoms are stronger and stick together better, other atoms, like those in water or air, are like super strong blocks that hold together really well. They don’t like to change as much, so they are usually more stable.




Seriously, metals require ions to stabilize in various contexts, primarily due to their tendency to lose electrons and form positive charges, the ions in PBS can neutralize the positive charge of the copper ions, reducing the likelihood of oxidation or other reactions that would destabilize the copper. PBS also maintains a stable pH, which is important for keeping copper in its soluble form. In bac water or regular saline, changes in pH can lead to precipitation or oxidation of copper ions. (5 to 7 for bac and reg saline vs 7.2 to 7.4 for Phosphate Buffered Saline)

Hence why GHK-Cu needs PBS (again because the ionic solution (has a lot of ions) stabilizes the added copper), otherwise the copper-nitrogen bonds are likely to break.
View attachment 3276079


The common folk seems to be dissolving GHK-Cu in Bac Water since that is the usual commercially available and promoted solvent for peptides².
However, Bac Water has a lower ion concentration than PBS (which makes the copper less likely to be stable), I hypothesize that the pain comes from the free copper atoms raping your tissues (not fully understood).

View attachment 3275864

Injection solutions :

Phosphate Buffered Saline :

https://sci-hub.ru/10.1172/JCI116842

View attachment 3275998

https://onlinelibrary.wiley.com/doi/epdf/10.1002/jor.22831

View attachment 3276003

https://sci-hub.ru/10.1046/j.1523-1747.1999.00606.x

View attachment 3276026


i.e. what is mostly used in studies and what is recommended by CaymanChem

Making PBS at home looks annoying af so just fucking buy it : https://www.sigmaaldrich.com/US/en/product/sigma/d8537

WORKS AND STABLE



Normale saline (0.9% saline) :

https://d1wqtxts1xzle7.cloudfront.net/37163377/Massey_Patt_Aoust_The_Effect_of_GHK-Cu_on_the_Healing_of_Diabet_Ulcers_A_Pilot_Sutyd_WOUNDS_4_21-28_1992-libre.PDF?1427753974=&response-content-disposition=attachment%3B+filename%3DMassey_Patt_Aoust_The_Effect_of_GHK_Cu_o.pdf&Expires=1730648621&Signature=bEi6x2QXzs~nLvVNqdEnYmv7~ruaYJ3wGxGnHPUBnbcUmupFy9OMJu7w2M8FOsNd6ksfpzr4TNj-7184G5MT1rk224QmlJ37S6yYnjzsLTWhqjIZqtcp~Lsf6Qy~xxDEnhMbmh~DjIE891q1xoUH4l6V73w5kDgB3C-e~V06E1gnd9vJi0sdDLj5Eo0gGSVBWxV3fF2IF7cfHo9l36YXHJgRoFpOyyYKZjlF3rN-dWJ6C7VRRq8iAz8YgtCExeNyzDJA0l0HYIjE5xS65171U10rPPBRcZ5aOFzhieuFk7awwfSbKg5COwUvLmUzf9RyiTSZKci8BhSDDSbQYj6h-A__&Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA

Direct on-site injection tho

View attachment 3276031


View attachment 3276018View attachment 3276019

WORKS BUT THEORETICALLY NOT STABLE, since the PH will be too low

You'd need to adjsut it yourself⁷

View attachment 3276064

BAC WATER :

DOESN'T WORK (YOU ONLY GET GHK AND INJECTION SITE DAMAGE)²




Topical :

Topical formulations of GHKcu have been developed and are effective

BetTar:

The best one (both low-cost, most effective and most stable) being Bet Tar which is a recent (2023) chinese developped ionic-liquid-based delivery vehicle composed of betaine and tartaric acid

View attachment 3276085

View attachment 3276086
View attachment 3276090

Stable as fuck :
View attachment 3276087

PDI is dispersity

I will be attaching the papers⁸ in case President Xi takes them down

Hyaluronic acid-based hydrogel embedded with GHK peptide nanofibers : ( )

Works af for direct wound healing

In situ photo-crosslinkable hyaluronic acid-based hydrogel embedded with GHK peptide nanofibers for bioactive wound healing

A versatile hydrogel was developed for enhancing bioactive wound healing by introducing the amphiphilic GHK peptide (GHK-C16) into a photo-crosslinkab…

www.sciencedirect.com

View attachment 3276113

YES BRO I WILL TOTALLY MAKE THIS jfl

View attachment 3276114


THERE ARE 8 BILLION GOOD TOPICAL ways but WE CAN NOT MAKE/BUY any of these

This was good for wound closure but go figure how they made it

https://sci-hub.ru/10.1046/j.1524-475X.1994.20406.x

View attachment 3276058View attachment 3276117

Concering DMSO :

IT DOESN'T WORK (IT'S EVEN LESS STABLE THAN BAC WATER since it has less ions (water (bac water) has H+ and OH- (water)))

These niggas tried to study its structure while dissolving it in DMSO¹⁰ : https://sci-hub.ru/10.1016/0162-0134(89)84011-5, which gave them completly retarded results "under their conditions"

Metals in general seem to be struggling in DMSO
View attachment 3276159

Goodluck rabbitholemaxxing this

https://sci-hub.ru/10.1016/j.ccr.2004.02.005

TLDR :



- RECONSTITUTE GHK-Cu IN PBS or DPBS (which you can buy) and inject it daily

- BUY and RECONSTITUTE GHK IN BAC WATER IF YOU ONLY WANT COLLAGEN BOOST AND EASE OF USE and inject it daily

- TRY TO MAKE a [Bet] [Tar] GHK-Cu Topical Liquid Solution, if you want, for when you can't inject or as first-aid

Spoiler: Tags

@Hexmask @N1666 @Clavicular @NorwoodAscender @meathead

¹Pickart, L., & Margolina, A. (2018). Regenerative and protective actions of the GHK-Cu peptide in the light of the new gene data. International Journal of Molecular Sciences, 19(7), https://pmc.ncbi.nlm.nih.gov/articles/PMC6073405/pdf/ijms-19-01987.pdf
²
³Pickart L, Thaler MM. Tripeptide in human serum which prolongs survival of normal liver cells and stimulates growth in neoplastic liver. Nat New Biol. 1973 May 16;243(124):85-7. PMID: 4349963.
⁴Maquart, F.-X., Pickart, L., Laurent, M., Gillery, P., Monboisse, J.-C., & Borel, J.-P. (1988). Stimulation of collagen synthesis in fibroblast cultures by the tripeptide-copper complex glycyl-L-histidyl-L-lysine-Cu²⁺. Biochemical and Biophysical Research Communications, 238(2), 343-346. https://sci-hub.ru/10.1016/0014-5793(88)80509-X
⁵ Google it and add Reddit and various forums to the key-words
⁶Siméon, A., Emonard, H., Hornebeck, W., & Maquart, F.-X. (2000). The tripeptide-copper complex glycyl-L-histidyl-L-lysine-Cu²⁺ stimulates matrix metalloproteinase-2 expression by fibroblast cultures. Life Sciences, 67(20), 2257-2265. https://sci-hub.ru/10.1016/S0024-3205(00)00803-1
⁷ Pickart, L. R. (1992). Method of using copper(II) containing compounds to accelerate wound healing (U.S. Patent No. 5164367). U.S. Patent and Trademark Office. https://patentimages.storage.googleapis.com/28/c1/c7/6fb3bbcf61becb/US5164367.pdf
⁸Liu, T., Hu, L., Lu, B., Bo, Y., Liao, Y., Zhan, J., Pei, Y., Sun, H., Wang, Z., Guo, C., & Zhang, J. (2023). A novel delivery vehicle for copper peptides. New Journal of Chemistry https://pubs.rsc.org/en/content/articlelanding/2023/nj/d2nj04282g#fn1
⁹L. Pickart, in BioIogy of Copper Complexes, J. R. C. Sorenson, Ed., Human Press, Clifton, NJ, 1987, pp. 273-285 (https://library.lol/main/04A4B0236383B58B30E1AF569542355F), neurogenesis also mentionned here¹⁰
¹⁰Antholine, W. E., Petering, D. H., & Pickart, L. (1989). ESR studies of the interaction of copper(II) GHK, histidine, and Ehrlich cells. Journal of Inorganic Biochemistry, 35(2), U-224
¹¹Pickart, L., Vasquez-Soltero, J. M., & Margolina, A. (2012). The human tripeptide GHK-Cu in prevention of oxidative stress and degenerative conditions of aging: Implications for cognitive health. Oxidative Medicine and Cellular Longevity, 2012, Article ID 324832. https://doi.org/10.1155/2012/324832

basically dont use bac water with ghk-cu use BetTar

 

[CloudyCuck]

I read all. Does it help with acne scars? Any long term health effects?

 

[norwoodingmanlet]

I read all. Does it help with acne scars? Any long term health effects?

eh

acne scars go for

co2 laser
chemical peels
microneedling

this guide is okay but do not forget pbs is NOT stable or sterile after the peptide is added so youll want to ideally use the vial quick

my theory would be just somehow use your 50 mg vials in a week

 

[supernova..]

Mirin , great thread will read every word later

 

[User28823]

holy holy holy

but what about this

 

[User28823]

SON

THIS IS EXACTLY WHAT I WAS LOOKING FOR

HIGH IQ ASF

WE ARE ALL SPAMMIN GHK-CU

@Saint Casanova @Tyler1 @xevuxia

 

[iphone]

good shit solid advice here

 

[notagrey]

GHK-Cu, on paper, seems to be the single best looksmaxxing compound there is (perfect skin, anti-aging, surgery recovery, neurogenesis⁹,¹¹ etc)¹, yet no one seems to be reaping any of its benefits. What’s even more bizarre is that injection-related pain is massively reported, more consistently so than its supposed benefits².

Quickly, Glycyl-L-histidyl-L-lysine (GHK) is a tripeptide isolated from human plasma³, which possesses a high affinity for copper (II) ions, with which it spontaneously forms a complex (GHK-Cu). GHK alone has been shown to be responsible for the collagen boost⁴ and no injection-related pain has been reported with it⁵, so go for it if you want but it's the added copper that is responsible for this myriad of blessings;

EXAMPLES :
The complex, unlike GHK alone, helps activate matrix metalloproteinases, which break down damaged tissue so it can be replaced with healthy tissue⁶.

View attachment 3275853

OR

Compositions of copper (II) containing compounds have been show to accelerate the healing of wounds in warm-blooded animals, and more specifically the systemic loading of copper (II) compounds to accelerate the rate of wound healing following injury or surgery to warm-blooded animals.⁷

View attachment 3275859


; ...and consequently what seems to cause tissue damage during injection.


If we look at the cayman chemicals reviews of the molecules in question :


View attachment 3275820View attachment 3275821


The main differences are the addition of a copper atom and a significantly worse solubility profile, which suggests a causal relationship between these two differences (meaning that the added copper induces the worse solubility).

In fact, we know that metals are gay

Spoiler: Gay

Gay meaning they have different bonding properties vs carbon, oxygen, etc (this whole field of gay metals is “inorganic chemistry”) The reason for this is that metals basically have up to ten relevant electrons , which have comically complex geometries, are much larger (radius) and less bound to the nucelus

View attachment 3275870

Simply, they like to move a lot, they're playful building blocks that like to change and mix , while other atoms are stronger and stick together better, other atoms, like those in water or air, are like super strong blocks that hold together really well. They don’t like to change as much, so they are usually more stable.




Seriously, metals require ions to stabilize in various contexts, primarily due to their tendency to lose electrons and form positive charges, the ions in PBS can neutralize the positive charge of the copper ions, reducing the likelihood of oxidation or other reactions that would destabilize the copper. PBS also maintains a stable pH, which is important for keeping copper in its soluble form. In bac water or regular saline, changes in pH can lead to precipitation or oxidation of copper ions. (5 to 7 for bac and reg saline vs 7.2 to 7.4 for Phosphate Buffered Saline)

Hence why GHK-Cu needs PBS (again because the ionic solution (has a lot of ions) stabilizes the added copper), otherwise the copper-nitrogen bonds are likely to break.
View attachment 3276079


The common folk seems to be dissolving GHK-Cu in Bac Water since that is the usual commercially available and promoted solvent for peptides².
However, Bac Water has a lower ion concentration than PBS (which makes the copper less likely to be stable), I hypothesize that the pain comes from the free copper atoms raping your tissues (not fully understood).

View attachment 3275864

Injection solutions :

Phosphate Buffered Saline :

https://sci-hub.ru/10.1172/JCI116842

View attachment 3275998

https://onlinelibrary.wiley.com/doi/epdf/10.1002/jor.22831

View attachment 3276003

https://sci-hub.ru/10.1046/j.1523-1747.1999.00606.x

View attachment 3276026


i.e. what is mostly used in studies and what is recommended by CaymanChem

Making PBS at home looks annoying af so just fucking buy it : https://www.sigmaaldrich.com/US/en/product/sigma/d8537

WORKS AND STABLE



Normale saline (0.9% saline) :

https://d1wqtxts1xzle7.cloudfront.net/37163377/Massey_Patt_Aoust_The_Effect_of_GHK-Cu_on_the_Healing_of_Diabet_Ulcers_A_Pilot_Sutyd_WOUNDS_4_21-28_1992-libre.PDF?1427753974=&response-content-disposition=attachment%3B+filename%3DMassey_Patt_Aoust_The_Effect_of_GHK_Cu_o.pdf&Expires=1730648621&Signature=bEi6x2QXzs~nLvVNqdEnYmv7~ruaYJ3wGxGnHPUBnbcUmupFy9OMJu7w2M8FOsNd6ksfpzr4TNj-7184G5MT1rk224QmlJ37S6yYnjzsLTWhqjIZqtcp~Lsf6Qy~xxDEnhMbmh~DjIE891q1xoUH4l6V73w5kDgB3C-e~V06E1gnd9vJi0sdDLj5Eo0gGSVBWxV3fF2IF7cfHo9l36YXHJgRoFpOyyYKZjlF3rN-dWJ6C7VRRq8iAz8YgtCExeNyzDJA0l0HYIjE5xS65171U10rPPBRcZ5aOFzhieuFk7awwfSbKg5COwUvLmUzf9RyiTSZKci8BhSDDSbQYj6h-A__&Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA

Direct on-site injection tho

View attachment 3276031


View attachment 3276018View attachment 3276019

WORKS BUT THEORETICALLY NOT STABLE, since the PH will be too low

You'd need to adjsut it yourself⁷

View attachment 3276064

BAC WATER :

DOESN'T WORK (YOU ONLY GET GHK AND INJECTION SITE DAMAGE)²




Topical :

Topical formulations of GHKcu have been developed and are effective

BetTar:

The best one (both low-cost, most effective and most stable) being Bet Tar which is a recent (2023) chinese developped ionic-liquid-based delivery vehicle composed of betaine and tartaric acid

View attachment 3276085

View attachment 3276086
View attachment 3276090

Stable as fuck :
View attachment 3276087

PDI is dispersity

I will be attaching the papers⁸ in case President Xi takes them down

Hyaluronic acid-based hydrogel embedded with GHK peptide nanofibers : ( )

Works af for direct wound healing

In situ photo-crosslinkable hyaluronic acid-based hydrogel embedded with GHK peptide nanofibers for bioactive wound healing

A versatile hydrogel was developed for enhancing bioactive wound healing by introducing the amphiphilic GHK peptide (GHK-C16) into a photo-crosslinkab…

www.sciencedirect.com

View attachment 3276113

YES BRO I WILL TOTALLY MAKE THIS jfl

View attachment 3276114


THERE ARE 8 BILLION GOOD TOPICAL ways but WE CAN NOT MAKE/BUY any of these

This was good for wound closure but go figure how they made it

https://sci-hub.ru/10.1046/j.1524-475X.1994.20406.x

View attachment 3276058View attachment 3276117

Concering DMSO :

IT DOESN'T WORK (IT'S EVEN LESS STABLE THAN BAC WATER since it has less ions (water (bac water) has H+ and OH- (water)))

These niggas tried to study its structure while dissolving it in DMSO¹⁰ : https://sci-hub.ru/10.1016/0162-0134(89)84011-5, which gave them completly retarded results "under their conditions"

Metals in general seem to be struggling in DMSO
View attachment 3276159

Goodluck rabbitholemaxxing this

https://sci-hub.ru/10.1016/j.ccr.2004.02.005

TLDR :



- RECONSTITUTE GHK-Cu IN PBS or DPBS (which you can buy) and inject it daily

- BUY and RECONSTITUTE GHK IN BAC WATER IF YOU ONLY WANT COLLAGEN BOOST AND EASE OF USE and inject it daily

- TRY TO MAKE a [Bet] [Tar] GHK-Cu Topical Liquid Solution, if you want, for when you can't inject or as first-aid

Spoiler: Tags

@Hexmask @N1666 @Clavicular @NorwoodAscender @meathead

¹Pickart, L., & Margolina, A. (2018). Regenerative and protective actions of the GHK-Cu peptide in the light of the new gene data. International Journal of Molecular Sciences, 19(7), https://pmc.ncbi.nlm.nih.gov/articles/PMC6073405/pdf/ijms-19-01987.pdf
²
³Pickart L, Thaler MM. Tripeptide in human serum which prolongs survival of normal liver cells and stimulates growth in neoplastic liver. Nat New Biol. 1973 May 16;243(124):85-7. PMID: 4349963.
⁴Maquart, F.-X., Pickart, L., Laurent, M., Gillery, P., Monboisse, J.-C., & Borel, J.-P. (1988). Stimulation of collagen synthesis in fibroblast cultures by the tripeptide-copper complex glycyl-L-histidyl-L-lysine-Cu²⁺. Biochemical and Biophysical Research Communications, 238(2), 343-346. https://sci-hub.ru/10.1016/0014-5793(88)80509-X
⁵ Google it and add Reddit and various forums to the key-words
⁶Siméon, A., Emonard, H., Hornebeck, W., & Maquart, F.-X. (2000). The tripeptide-copper complex glycyl-L-histidyl-L-lysine-Cu²⁺ stimulates matrix metalloproteinase-2 expression by fibroblast cultures. Life Sciences, 67(20), 2257-2265. https://sci-hub.ru/10.1016/S0024-3205(00)00803-1
⁷ Pickart, L. R. (1992). Method of using copper(II) containing compounds to accelerate wound healing (U.S. Patent No. 5164367). U.S. Patent and Trademark Office. https://patentimages.storage.googleapis.com/28/c1/c7/6fb3bbcf61becb/US5164367.pdf
⁸Liu, T., Hu, L., Lu, B., Bo, Y., Liao, Y., Zhan, J., Pei, Y., Sun, H., Wang, Z., Guo, C., & Zhang, J. (2023). A novel delivery vehicle for copper peptides. New Journal of Chemistry https://pubs.rsc.org/en/content/articlelanding/2023/nj/d2nj04282g#fn1
⁹L. Pickart, in BioIogy of Copper Complexes, J. R. C. Sorenson, Ed., Human Press, Clifton, NJ, 1987, pp. 273-285 (https://library.lol/main/04A4B0236383B58B30E1AF569542355F), neurogenesis also mentionned here¹⁰
¹⁰Antholine, W. E., Petering, D. H., & Pickart, L. (1989). ESR studies of the interaction of copper(II) GHK, histidine, and Ehrlich cells. Journal of Inorganic Biochemistry, 35(2), U-224
¹¹Pickart, L., Vasquez-Soltero, J. M., & Margolina, A. (2012). The human tripeptide GHK-Cu in prevention of oxidative stress and degenerative conditions of aging: Implications for cognitive health. Oxidative Medicine and Cellular Longevity, 2012, Article ID 324832. https://doi.org/10.1155/2012/324832

I dont plan on injecting, how would one make a topical serum

 

[dino125]

ghk is cope buddy boyos

 

[TheAnimal]

Another way to buy without verification: https://www.ebay.com/itm/387005608544

thank you, exactly what i was looking for, guy linked a website where you have to be part of an org to buy it

 

[aimbotuser3123]

Guys what should I use GHK-CU or Tretinoin