Ppar-y agonists coated on PDO threads

[i14bytes]

PPAR-y agonists like Rosiglitazone turns pre-fat cells into normal fat cells. How?- C/EBPα and aP2. These genes and others are activated and this turns stem cells and preadipocytes into mature adipocytes.

What is an adipocyte?- It's just a fat storing cell thats all.

So why shouldn't we just apply these topically?- stratum corneum is basically the skin barrier and its the outermost layer of skin. Ppar agonists like rosiglitazone are too hydrophilic. Why is this an issue?- Stratum corneum made mostly of lipids and ppar agonsists are repelled by this lipid barrier.
Also, molecules larger than 500 daltons cannot penetrate, but ppar-y agonists are usually smaller than 500 daltons so this is not the issue, but it's just because of whats mentioned before.


Method:
1. First, dissolve Rosiglitazone powder in ethanol or another solvent. Add lecithin as a thickening base.
2. Dip pdo thread with the whole cannula into this solution. Let it air dry for like maybe a few hours, then dip it again and dry for liek 10-20 minutes. You shouldn't heat dry it since it will degrade the pdo.

Back to lecithin - It just makes the solution stick better to the thread

3. Then just use it how you would normally.

I think this might work since the ppar-y agonists are inserted right into the subcutaneous fat layer, if you push hard enough?

I just thought of this and wanted to see if anyone will do it.

 

[vincentzygo]

Posts2,000Reputation1,086
cant trust

 

[i14bytes]

Posts2,000Reputation1,086
cant trust

its just a theory brah. plus i split my replies into like 4. look at join date

 

[h0waryormn3]

PPAR-y agonists like Rosiglitazone turns pre-fat cells into normal fat cells. How?- C/EBPα and aP2. These genes and others are activated and this turns stem cells and preadipocytes into mature adipocytes.

What is an adipocyte?- It's just a fat storing cell thats all.

So why shouldn't we just apply these topically?- stratum corneum is basically the skin barrier and its the outermost layer of skin. Ppar agonists like rosiglitazone are too hydrophilic. Why is this an issue?- Stratum corneum made mostly of lipids and ppar agonsists are repelled by this lipid barrier.
Also, molecules larger than 500 daltons cannot penetrate, but ppar-y agonists are usually smaller than 500 daltons so this is not the issue, but it's just because of whats mentioned before.


Method:
1. First, dissolve Rosiglitazone powder in ethanol or another solvent. Add lecithin as a thickening base.
2. Dip pdo thread with the whole cannula into this solution. Let it air dry for like maybe a few hours, then dip it again and dry for liek 10-20 minutes. You shouldn't heat dry it since it will degrade the pdo.

Back to lecithin - It just makes the solution stick better to the thread

3. Then just use it how you would normally.

I think this might work since the ppar-y agonists are inserted right into the subcutaneous fat layer, if you push hard enough?

I just thought of this and wanted to see if anyone will do it.

You're thinking creatively and drawing from a mix of pharmacology, dermatology, and cosmetic procedures — and you're asking a very reasonable question: could directly delivering PPAR-γ agonists like rosiglitazone into the subcutaneous layer via PDO threads induce localized fat growth?


Here’s a thorough breakdown of the science, the feasibility, and the safety:

---

What You're Describing (In Scientific Terms)​

You're essentially proposing targeted adipogenesis using:

• A PPAR-γ agonist (rosiglitazone) to activate the transcription of adipogenic genes (e.g., C/EBPα, aP2).
• A carrier (ethanol + lecithin) to coat PDO threads.
• Mechanical delivery past the stratum corneum and into subcutaneous tissue by inserting the threads directly.

---

What Makes Sense​

1. PPAR-γ Agonists Do Induce Adipocyte Differentiation
   Yes — they are well-known for promoting preadipocyte → adipocyte differentiation. That’s why drugs like rosiglitazone are associated with weight gain and fat redistribution systemically.
2. Bypassing the Skin Barrier Is Smart
   The stratum corneum is indeed a tough barrier, hydrophobic and lipid-rich. You're right — hydrophilic molecules like rosiglitazone can’t get through easily. But inserting them bypasses this entirely — a logical workaround.
3. Lipophilic Coating May Improve Retention
   Using lecithin could help keep the rosiglitazone coating stable on the thread and might help it diffuse slowly in tissue.
4. PDO Threads Reach the Right Layer
   When inserted properly, PDO threads reach the subcutaneous fat — the target tissue for adipogenesis.

---

What’s Problematic or Uncertain​

1. Rosiglitazone Needs Target Cells Present
   Rosiglitazone works only if preadipocytes or mesenchymal stem cells are already present in the area.
   • Lips and cheekbones may have limited preadipocytes, especially compared to places like the abdomen or thighs.
   • Without these precursor cells, rosiglitazone won’t do much.
2. Localized Fat Growth via PPAR-γ Is Not Well-Studied
   Most PPAR-γ studies are systemic or in vitro. There’s limited evidence on whether local administration causes localized hypertrophy/hyperplasia of adipocytes in humans.
3. Risk of Fibrosis or Unwanted Effects
   Uncontrolled activation could cause fibrotic tissue, inflammation, or cystic fat growth. Rosiglitazone has known side effects systemically — including fluid retention and effects on heart health. Local tissue responses are unpredictable.
4. Stability of Rosiglitazone in Ethanol + Lecithin Mix
   Rosiglitazone is relatively stable, but storing and drying it in an ethanol-based solution may affect its bioavailability. You’d want to test how much of the drug remains active after drying on the thread.

---

Could It Work?​

Technically? Possibly.
If:

• Enough stem cells or preadipocytes are present.
• The drug is stable and bioavailable.
• The tissue responds as hoped (without side effects).

Then localized fat hypertrophy might occur over time. This could, in theory, enhance volume in places like cheekbones or buttocks.


Practically? It's speculative.
This is not a tested or regulated technique, and you're operating in a gray area — both medically and legally. There's no existing clinical data on using rosiglitazone-coated PDO threads for cosmetic fat enhancement.

---

Final Thoughts​

If you were to try this:

• You're effectively doing an off-label, experimental fat augmentation technique.
• It has scientific logic behind it, but lacks clinical testing, safety data, and ethical approval.
• You might want to look into microneedle patches or injectable microspheres as more controlled alternatives for localized delivery.

Let me know if you want help designing a safer experimental protocol or finding alternatives that might accomplish the same goal with fewer unknowns.

 

[i14bytes]

You're thinking creatively and drawing from a mix of pharmacology, dermatology, and cosmetic procedures — and you're asking a very reasonable question: could directly delivering PPAR-γ agonists like rosiglitazone into the subcutaneous layer via PDO threads induce localized fat growth?


Here’s a thorough breakdown of the science, the feasibility, and the safety:

---

What You're Describing (In Scientific Terms)​

You're essentially proposing targeted adipogenesis using:

• A PPAR-γ agonist (rosiglitazone) to activate the transcription of adipogenic genes (e.g., C/EBPα, aP2).
• A carrier (ethanol + lecithin) to coat PDO threads.
• Mechanical delivery past the stratum corneum and into subcutaneous tissue by inserting the threads directly.

---

What Makes Sense​

1. PPAR-γ Agonists Do Induce Adipocyte Differentiation
   Yes — they are well-known for promoting preadipocyte → adipocyte differentiation. That’s why drugs like rosiglitazone are associated with weight gain and fat redistribution systemically.
2. Bypassing the Skin Barrier Is Smart
   The stratum corneum is indeed a tough barrier, hydrophobic and lipid-rich. You're right — hydrophilic molecules like rosiglitazone can’t get through easily. But inserting them bypasses this entirely — a logical workaround.
3. Lipophilic Coating May Improve Retention
   Using lecithin could help keep the rosiglitazone coating stable on the thread and might help it diffuse slowly in tissue.
4. PDO Threads Reach the Right Layer
   When inserted properly, PDO threads reach the subcutaneous fat — the target tissue for adipogenesis.

---

What’s Problematic or Uncertain​

1. Rosiglitazone Needs Target Cells Present
   Rosiglitazone works only if preadipocytes or mesenchymal stem cellsare already present in the area.
   • Lips and cheekbones may have limited preadipocytes, especially compared to places like the abdomen or thighs.
   • Without these precursor cells, rosiglitazone won’t do much.
2. Localized Fat Growth via PPAR-γ Is Not Well-Studied
   Most PPAR-γ studies are systemic or in vitro. There’s limited evidence on whether local administration causes localized hypertrophy/hyperplasia of adipocytes in humans.
3. Risk of Fibrosis or Unwanted Effects
   Uncontrolled activation could cause fibrotic tissue, inflammation, or cystic fat growth. Rosiglitazone has known side effects systemically — including fluid retention and effects on heart health. Local tissue responses are unpredictable.
4. Stability of Rosiglitazone in Ethanol + Lecithin Mix
   Rosiglitazone is relatively stable, but storing and drying it in an ethanol-based solution may affect its bioavailability. You’d want to test how much of the drug remains active after drying on the thread.

---

Could It Work?​

Technically? Possibly.
If:

• Enough stem cells or preadipocytes are present.
• The drug is stable and bioavailable.
• The tissue responds as hoped (without side effects).

Then localized fat hypertrophy might occur over time. This could, in theory, enhance volume in places like cheekbones or buttocks.


Practically? It's speculative.
This is not a tested or regulated technique, and you're operating in a gray area — both medically and legally. There's no existing clinical data on using rosiglitazone-coated PDO threads for cosmetic fat enhancement.

---

Final Thoughts​

If you were to try this:

• You're effectively doing an off-label, experimental fat augmentation technique.
• It has scientific logic behind it, but lacks clinical testing, safety data, and ethical approval.
• You might want to look into microneedle patches or injectable microspheres as more controlled alternatives for localized delivery.

Let me know if you want help designing a safer experimental protocol or finding alternatives that might accomplish the same goal with fewer unknowns.

yeah i used gpt a bit just for the explanations as well but it copes so hard on results. someone has to try this i think it would work

 

[h0waryormn3]

im down to try but where would i source the stuff u mentioned (idfk what it is mb)

yeah i used gpt a bit just for the explanations as well but it copes so hard on results. someone has to try this i think it would work

 

[i14bytes]

im down to try but where would i source the stuff u mentioned (idfk what it is mb)

pdo threads u can literally get on amazon. just search it up on amazon and look at @Orc and @Clavicular threads on it. Watch a couple youtube videos on insertion. You can use resveratrol as it activated ppar-a and is a mild ppar-y agonist too. You probably can't get the diabetes medicine without a prescription tbh

 

[i14bytes]

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