How to properly use GHK-Cu

A year of bac water injections.... brutal

anyway it definitely works as intended but I suppose I'm not getting the gene (+/-) related benefits?
 
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A year of bac water injections.... brutal

anyway it definitely works as intended but I suppose I'm not getting the gene (+/-) related benefits?
Yes you injected GHK,, which still boosts collagen

1730781052447
,

but not GHK-Cu or in insufficient amounts (most of it was busy causing the bruising on the injection site)

Most of the benefits though are from GHK-Cu yes
 
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but not GHK-Cu or in insufficient amounts (most of it was busy causing the bruising on the injection site)
I never got brusing because I usually dilute it heavily with bac water.

How do I make PBS?
 
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Bro @NZb6Air remember how i told u i was planning on reconstituting it into a nasal . This is how i use my mt2 and it works fucking phenomenally . So ghk + bac ?
 
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I never got brusing because I usually dilute it heavily with bac water.

How do I make PBS?
I replied on dc
Bro @NZb6Air remember how i told u i was planning on reconstituting it into a nasal . This is how i use my mt2 and it works fucking phenomenally . So ghk + bac ?
ye ghk + bac injection for a lil or pbs + ghkcu injection for a lot , you could do pbs+ghkcu intranasally i guess? but you'd need insane doses in theory, look at hexarelin dosages for example https://sci-hub.se/10.1530/eje.0.1350407
 
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Coming in clutch after I'll have a nw7 hairline and not a single collagen protein on my face from permablasting TTM 15->25
 
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And ghkcu totally kills your IGF1 which is unfortunate
Tales. GHK-cu signals lowering of IGF-1 specifically in inflammatory responses in the body primarily of the expression in lung tissue to prevent bleomycin-induced pulmonary fibrosis. Stupid nigger. There's ZERO studies on any sort of systemic lowering of IGF-1. I've ran this peptide and encountered ZERO issues.
 
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Tales. GHK-cu signals lowering of IGF-1 specifically in inflammatory responses in the body primarily of the expression in lung tissue to prevent bleomycin-induced pulmonary fibrosis. Stupid nigger. There's ZERO studies on any sort of systemic lowering of IGF-1. I've ran this peptide and encountered ZERO issues.
it does

1730825398118
 
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There’s zero studies claiming as to what degree though so when you dig in further you see what I was talking about. I even used Ai to go through studies. Absolutely nothing. If I had to guess it’s to no noticeable degree. But when in regards to inflammation that’s beneficial. Inflammation influences cancer.
 
GHK-Cu, on paper, seems to be the single best looksmaxxing compound there is (perfect skin, anti-aging, surgery recovery, neurogenesis,¹¹ etc)¹, yet no one seems to be reaping any of its benefits. What’s even more bizarre is that injection-related pain is massively reported, more consistently so than its supposed benefits².

a white cat is sitting on a box with the words `` why '' written on it .'' written on it .




Quickly, Glycyl-L-histidyl-L-lysine (GHK) is a tripeptide isolated from human plasma³, which possesses a high affinity for copper (II) ions, with which it spontaneously forms a complex (GHK-Cu). GHK alone has been shown to be responsible for the collagen boost⁴ and no injection-related pain has been reported with it⁵, so go for it if you want but it's the added copper that is responsible for this myriad of blessings;

EXAMPLES :
The complex, unlike GHK alone, helps activate matrix metalloproteinases, which break down damaged tissue so it can be replaced with healthy tissue⁶.


View attachment 3275853

OR

Compositions of copper (II) containing compounds have been show to accelerate the healing of wounds in warm-blooded animals, and more specifically the systemic loading of copper (II) compounds to accelerate the rate of wound healing following injury or surgery to warm-blooded animals.⁷


View attachment 3275859


; ...and consequently what seems to cause tissue damage during injection.


If we look at the cayman chemicals reviews of the molecules in question :



View attachment 3275820View attachment 3275821


The main differences are the addition of a copper atom and a significantly worse solubility profile, which suggests a causal relationship between these two differences (meaning that the added copper induces the worse solubility).

In fact, we know that metals are gay

Gay meaning they have different bonding properties vs carbon, oxygen, etc (this whole field of gay metals is “inorganic chemistry”) The reason for this is that metals basically have up to ten relevant electrons , which have comically complex geometries, are much larger (radius) and less bound to the nucelus

View attachment 3275870

Simply, they like to move a lot, they're playful building blocks that like to change and mix :heart:, while other atoms are stronger and stick together better, 🥺 other atoms, like those in water or air, are like super strong blocks that hold together really well🍆. They don’t like to change as much, so they are usually more stable. :eek:




Seriously, metals require ions to stabilize in various contexts, primarily due to their tendency to lose electrons and form positive charges, the ions in PBS can neutralize the positive charge of the copper ions, reducing the likelihood of oxidation or other reactions that would destabilize the copper. PBS also maintains a stable pH, which is important for keeping copper in its soluble form. In bac water or regular saline, changes in pH can lead to precipitation or oxidation of copper ions. (5 to 7 for bac and reg saline vs 7.2 to 7.4 for Phosphate Buffered Saline)

Hence why GHK-Cu needs PBS (again because the ionic solution (has a lot of ions) stabilizes the added copper), otherwise the copper-nitrogen bonds are likely to break.
View attachment 3276079


The common folk seems to be dissolving GHK-Cu in Bac Water since that is the usual commercially available and promoted solvent for peptides².
However, Bac Water has a lower ion concentration than PBS (which makes the copper less likely to be stable), I hypothesize that the pain comes from the free copper atoms raping your tissues (not fully understood).


View attachment 3275864

Injection solutions :

Phosphate Buffered Saline :


View attachment 3275998


View attachment 3276003


View attachment 3276026


i.e. what is mostly used in studies and what is recommended by CaymanChem

Making PBS at home looks annoying af so just fucking buy it : https://www.sigmaaldrich.com/US/en/product/sigma/d8537

WORKS AND STABLE




Normale saline (0.9% saline) :


Direct on-site injection tho

View attachment 3276031


View attachment 3276018View attachment 3276019

WORKS BUT THEORETICALLY NOT STABLE, since the PH will be too low

You'd need to adjsut it yourself⁷


View attachment 3276064

BAC WATER :

DOESN'T WORK (YOU ONLY GET GHK AND INJECTION SITE DAMAGE)²





Topical :

Topical formulations of GHKcu have been developed and are effective

BetTar:

The best one (both low-cost, most effective and most stable) being Bet Tar which is a recent (2023) chinese developped ionic-liquid-based delivery vehicle composed of betaine and tartaric acid
https://external-content.duckduckgo.com/iu/?u=https%3A%2F%2Fmedia1.tenor.com%2Fm%2F_ylM1V0OJlIAAAAC%2Fdonald-trump-talking.gif&f=1&nofb=1&ipt=7ecca7ab4663a5d1e0dd8f1ccd4a7e406e04e4a17d62f94524b064be533188e0&ipo=images


View attachment 3276085

View attachment 3276086
View attachment 3276090

Stable as fuck :
View attachment 3276087

PDI is dispersity

I will be attaching the papers⁸ in case President Xi takes them down


Hyaluronic acid-based hydrogel embedded with GHK peptide nanofibers : ( :lul: )

Works af for direct wound healing


View attachment 3276113

YES BRO I WILL TOTALLY MAKE THIS jfl

View attachment 3276114


THERE ARE 8 BILLION GOOD TOPICAL ways but WE CAN NOT MAKE/BUY any of these

This was good for wound closure but go figure how they made it

View attachment 3276058View attachment 3276117

Concering DMSO :

IT DOESN'T WORK (IT'S EVEN LESS STABLE THAN BAC WATER since it has less ions (water (bac water) has H+ and OH- (water)))

These niggas tried to study its structure while dissolving it in DMSO¹⁰ : https://sci-hub.ru/10.1016/0162-0134(89)84011-5, which gave them completly retarded results "under their conditions"

Metals in general seem to be struggling in DMSO

View attachment 3276159

Goodluck rabbitholemaxxing this :lul:








TLDR :



- RECONSTITUTE GHK-Cu IN PBS or DPBS (which you can buy) and inject it daily

- BUY and RECONSTITUTE GHK IN BAC WATER IF YOU ONLY WANT COLLAGEN BOOST AND EASE OF USE and inject it daily

- TRY TO MAKE a [Bet] [Tar] GHK-Cu Topical Liquid Solution, if you want, for when you can't inject or as first-aid





@Hexmask @N1666 @Clavicular @NorwoodAscender @meathead



¹Pickart, L., & Margolina, A. (2018). Regenerative and protective actions of the GHK-Cu peptide in the light of the new gene data. International Journal of Molecular Sciences, 19(7), https://pmc.ncbi.nlm.nih.gov/articles/PMC6073405/pdf/ijms-19-01987.pdf
²
³Pickart L, Thaler MM. Tripeptide in human serum which prolongs survival of normal liver cells and stimulates growth in neoplastic liver. Nat New Biol. 1973 May 16;243(124):85-7. PMID: 4349963.
⁴Maquart, F.-X., Pickart, L., Laurent, M., Gillery, P., Monboisse, J.-C., & Borel, J.-P. (1988). Stimulation of collagen synthesis in fibroblast cultures by the tripeptide-copper complex glycyl-L-histidyl-L-lysine-Cu²⁺. Biochemical and Biophysical Research Communications, 238(2), 343-346. https://sci-hub.ru/10.1016/0014-5793(88)80509-X
⁵ Google it and add Reddit and various forums to the key-words
⁶Siméon, A., Emonard, H., Hornebeck, W., & Maquart, F.-X. (2000). The tripeptide-copper complex glycyl-L-histidyl-L-lysine-Cu²⁺ stimulates matrix metalloproteinase-2 expression by fibroblast cultures. Life Sciences, 67(20), 2257-2265. https://sci-hub.ru/10.1016/S0024-3205(00)00803-1
⁷ Pickart, L. R. (1992). Method of using copper(II) containing compounds to accelerate wound healing (U.S. Patent No. 5164367). U.S. Patent and Trademark Office. https://patentimages.storage.googleapis.com/28/c1/c7/6fb3bbcf61becb/US5164367.pdf
⁸Liu, T., Hu, L., Lu, B., Bo, Y., Liao, Y., Zhan, J., Pei, Y., Sun, H., Wang, Z., Guo, C., & Zhang, J. (2023). A novel delivery vehicle for copper peptides. New Journal of Chemistry https://pubs.rsc.org/en/content/articlelanding/2023/nj/d2nj04282g#fn1
⁹L. Pickart, in BioIogy of Copper Complexes, J. R. C. Sorenson, Ed., Human Press, Clifton, NJ, 1987, pp. 273-285 (https://library.lol/main/04A4B0236383B58B30E1AF569542355F), neurogenesis also mentionned here¹⁰
¹⁰Antholine, W. E., Petering, D. H., & Pickart, L. (1989). ESR studies of the interaction of copper(II) GHK, histidine, and Ehrlich cells. Journal of Inorganic Biochemistry, 35(2), U-224
¹¹Pickart, L., Vasquez-Soltero, J. M., & Margolina, A. (2012). The human tripeptide GHK-Cu in prevention of oxidative stress and degenerative conditions of aging: Implications for cognitive health. Oxidative Medicine and Cellular Longevity, 2012, Article ID 324832. https://doi.org/10.1155/2012/324832

Holy what a book
 
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amazing thread, bump
 
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what are the negative effects of ghk-cu lowering igf-1 in fully grown manlets like myself?
 
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is this any good or naur :unsure:

CAS: 49557-75-7
Formula : C14H22N6O4Cu·C4H4O2
g/mol : 485.986
 
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4sd g
 
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https://hvyresearch.com/product/bacteriostatic-water-with-pbs/
Pretty sure the risk of infection would be pretty high with a typical distilled water PBS solution. Wouldn't be able to keep it for more than a couple days once opened (without re-sterilizing). IDK though. + mayb the bac water would fucc the benefits of PBS.
 
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Phosphate Buffered Saline :
1732844480414


Anyone tried PBS from Tracy?

I think they've had quality issues with BAC, and I'm not sure if that will carry over to PBS, too.

@NZb6Air
@halloweed
@Dyorotic.

Phosphate-buffered saline 15ml, pH= 6,96 // 10.
How do I make PBS?
 
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View attachment 3322380

Anyone tried PBS from Tracy?

I think they've had quality issues with BAC, and I'm not sure if that will carry over to PBS, too.

@NZb6Air
@halloweed
@Dyorotic.

Phosphate-buffered saline 15ml, pH= 6,96 // 10.
Yea I'm aware, in transit
 
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Reactions: halloweed and DORIAN
View attachment 3322380

Anyone tried PBS from Tracy?

I think they've had quality issues with BAC, and I'm not sure if that will carry over to PBS, too.

@NZb6Air
@halloweed
@Dyorotic.

Phosphate-buffered saline 15ml, pH= 6,96 // 10.
yeah we've all switched to PBS. Painless even when barely diluted
 
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Reactions: NZb6Air and DORIAN
GHK-Cu, on paper, seems to be the single best looksmaxxing compound there is (perfect skin, anti-aging, surgery recovery, neurogenesis,¹¹ etc)¹, yet no one seems to be reaping any of its benefits. What’s even more bizarre is that injection-related pain is massively reported, more consistently so than its supposed benefits².

a white cat is sitting on a box with the words `` why '' written on it .'' written on it .




Quickly, Glycyl-L-histidyl-L-lysine (GHK) is a tripeptide isolated from human plasma³, which possesses a high affinity for copper (II) ions, with which it spontaneously forms a complex (GHK-Cu). GHK alone has been shown to be responsible for the collagen boost⁴ and no injection-related pain has been reported with it⁵, so go for it if you want but it's the added copper that is responsible for this myriad of blessings;

EXAMPLES :
The complex, unlike GHK alone, helps activate matrix metalloproteinases, which break down damaged tissue so it can be replaced with healthy tissue⁶.


View attachment 3275853

OR

Compositions of copper (II) containing compounds have been show to accelerate the healing of wounds in warm-blooded animals, and more specifically the systemic loading of copper (II) compounds to accelerate the rate of wound healing following injury or surgery to warm-blooded animals.⁷


View attachment 3275859


; ...and consequently what seems to cause tissue damage during injection.


If we look at the cayman chemicals reviews of the molecules in question :



View attachment 3275820View attachment 3275821


The main differences are the addition of a copper atom and a significantly worse solubility profile, which suggests a causal relationship between these two differences (meaning that the added copper induces the worse solubility).

In fact, we know that metals are gay

Gay meaning they have different bonding properties vs carbon, oxygen, etc (this whole field of gay metals is “inorganic chemistry”) The reason for this is that metals basically have up to ten relevant electrons , which have comically complex geometries, are much larger (radius) and less bound to the nucelus

View attachment 3275870

Simply, they like to move a lot, they're playful building blocks that like to change and mix :heart:, while other atoms are stronger and stick together better, 🥺 other atoms, like those in water or air, are like super strong blocks that hold together really well🍆. They don’t like to change as much, so they are usually more stable. :eek:




Seriously, metals require ions to stabilize in various contexts, primarily due to their tendency to lose electrons and form positive charges, the ions in PBS can neutralize the positive charge of the copper ions, reducing the likelihood of oxidation or other reactions that would destabilize the copper. PBS also maintains a stable pH, which is important for keeping copper in its soluble form. In bac water or regular saline, changes in pH can lead to precipitation or oxidation of copper ions. (5 to 7 for bac and reg saline vs 7.2 to 7.4 for Phosphate Buffered Saline)

Hence why GHK-Cu needs PBS (again because the ionic solution (has a lot of ions) stabilizes the added copper), otherwise the copper-nitrogen bonds are likely to break.
View attachment 3276079


The common folk seems to be dissolving GHK-Cu in Bac Water since that is the usual commercially available and promoted solvent for peptides².
However, Bac Water has a lower ion concentration than PBS (which makes the copper less likely to be stable), I hypothesize that the pain comes from the free copper atoms raping your tissues (not fully understood).


View attachment 3275864

Injection solutions :

Phosphate Buffered Saline :


View attachment 3275998


View attachment 3276003


View attachment 3276026


i.e. what is mostly used in studies and what is recommended by CaymanChem

Making PBS at home looks annoying af so just fucking buy it : https://www.sigmaaldrich.com/US/en/product/sigma/d8537

WORKS AND STABLE




Normale saline (0.9% saline) :


Direct on-site injection tho

View attachment 3276031


View attachment 3276018View attachment 3276019

WORKS BUT THEORETICALLY NOT STABLE, since the PH will be too low

You'd need to adjsut it yourself⁷


View attachment 3276064

BAC WATER :

DOESN'T WORK (YOU ONLY GET GHK AND INJECTION SITE DAMAGE)²





Topical :

Topical formulations of GHKcu have been developed and are effective

BetTar:

The best one (both low-cost, most effective and most stable) being Bet Tar which is a recent (2023) chinese developped ionic-liquid-based delivery vehicle composed of betaine and tartaric acid
https://external-content.duckduckgo.com/iu/?u=https%3A%2F%2Fmedia1.tenor.com%2Fm%2F_ylM1V0OJlIAAAAC%2Fdonald-trump-talking.gif&f=1&nofb=1&ipt=7ecca7ab4663a5d1e0dd8f1ccd4a7e406e04e4a17d62f94524b064be533188e0&ipo=images


View attachment 3276085

View attachment 3276086
View attachment 3276090

Stable as fuck :
View attachment 3276087

PDI is dispersity

I will be attaching the papers⁸ in case President Xi takes them down


Hyaluronic acid-based hydrogel embedded with GHK peptide nanofibers : ( :lul: )

Works af for direct wound healing


View attachment 3276113

YES BRO I WILL TOTALLY MAKE THIS jfl

View attachment 3276114


THERE ARE 8 BILLION GOOD TOPICAL ways but WE CAN NOT MAKE/BUY any of these

This was good for wound closure but go figure how they made it

View attachment 3276058View attachment 3276117

Concering DMSO :

IT DOESN'T WORK (IT'S EVEN LESS STABLE THAN BAC WATER since it has less ions (water (bac water) has H+ and OH- (water)))

These niggas tried to study its structure while dissolving it in DMSO¹⁰ : https://sci-hub.ru/10.1016/0162-0134(89)84011-5, which gave them completly retarded results "under their conditions"

Metals in general seem to be struggling in DMSO

View attachment 3276159

Goodluck rabbitholemaxxing this :lul:








TLDR :



- RECONSTITUTE GHK-Cu IN PBS or DPBS (which you can buy) and inject it daily

- BUY and RECONSTITUTE GHK IN BAC WATER IF YOU ONLY WANT COLLAGEN BOOST AND EASE OF USE and inject it daily

- TRY TO MAKE a [Bet] [Tar] GHK-Cu Topical Liquid Solution, if you want, for when you can't inject or as first-aid





@Hexmask @N1666 @Clavicular @NorwoodAscender @meathead



¹Pickart, L., & Margolina, A. (2018). Regenerative and protective actions of the GHK-Cu peptide in the light of the new gene data. International Journal of Molecular Sciences, 19(7), https://pmc.ncbi.nlm.nih.gov/articles/PMC6073405/pdf/ijms-19-01987.pdf
²
³Pickart L, Thaler MM. Tripeptide in human serum which prolongs survival of normal liver cells and stimulates growth in neoplastic liver. Nat New Biol. 1973 May 16;243(124):85-7. PMID: 4349963.
⁴Maquart, F.-X., Pickart, L., Laurent, M., Gillery, P., Monboisse, J.-C., & Borel, J.-P. (1988). Stimulation of collagen synthesis in fibroblast cultures by the tripeptide-copper complex glycyl-L-histidyl-L-lysine-Cu²⁺. Biochemical and Biophysical Research Communications, 238(2), 343-346. https://sci-hub.ru/10.1016/0014-5793(88)80509-X
⁵ Google it and add Reddit and various forums to the key-words
⁶Siméon, A., Emonard, H., Hornebeck, W., & Maquart, F.-X. (2000). The tripeptide-copper complex glycyl-L-histidyl-L-lysine-Cu²⁺ stimulates matrix metalloproteinase-2 expression by fibroblast cultures. Life Sciences, 67(20), 2257-2265. https://sci-hub.ru/10.1016/S0024-3205(00)00803-1
⁷ Pickart, L. R. (1992). Method of using copper(II) containing compounds to accelerate wound healing (U.S. Patent No. 5164367). U.S. Patent and Trademark Office. https://patentimages.storage.googleapis.com/28/c1/c7/6fb3bbcf61becb/US5164367.pdf
⁸Liu, T., Hu, L., Lu, B., Bo, Y., Liao, Y., Zhan, J., Pei, Y., Sun, H., Wang, Z., Guo, C., & Zhang, J. (2023). A novel delivery vehicle for copper peptides. New Journal of Chemistry https://pubs.rsc.org/en/content/articlelanding/2023/nj/d2nj04282g#fn1
⁹L. Pickart, in BioIogy of Copper Complexes, J. R. C. Sorenson, Ed., Human Press, Clifton, NJ, 1987, pp. 273-285 (https://library.lol/main/04A4B0236383B58B30E1AF569542355F), neurogenesis also mentionned here¹⁰
¹⁰Antholine, W. E., Petering, D. H., & Pickart, L. (1989). ESR studies of the interaction of copper(II) GHK, histidine, and Ehrlich cells. Journal of Inorganic Biochemistry, 35(2), U-224
¹¹Pickart, L., Vasquez-Soltero, J. M., & Margolina, A. (2012). The human tripeptide GHK-Cu in prevention of oxidative stress and degenerative conditions of aging: Implications for cognitive health. Oxidative Medicine and Cellular Longevity, 2012, Article ID 324832. https://doi.org/10.1155/2012/324832

so all those products online claiming to be selling ghk cu peptides are just full of shit?
 

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