How to properly use GHK-Cu

[Deleted member 4633]

but not GHK-Cu or in insufficient amounts (most of it was busy causing the bruising on the injection site)

I never got brusing because I usually dilute it heavily with bac water.

How do I make PBS?

 

[Deleted member 19325]

Bro @NZb6Air remember how i told u i was planning on reconstituting it into a nasal . This is how i use my mt2 and it works fucking phenomenally . So ghk + bac ?

 

[NZb6Air]

I never got brusing because I usually dilute it heavily with bac water.

How do I make PBS?

I replied on dc

Bro @NZb6Air remember how i told u i was planning on reconstituting it into a nasal . This is how i use my mt2 and it works fucking phenomenally . So ghk + bac ?

ye ghk + bac injection for a lil or pbs + ghkcu injection for a lot , you could do pbs+ghkcu intranasally i guess? but you'd need insane doses in theory, look at hexarelin dosages for example https://sci-hub.se/10.1530/eje.0.1350407

 

[MA_ascender]

Coming in clutch after I'll have a nw7 hairline and not a single collagen protein on my face from permablasting TTM 15->25

 

[chincel11]

Can you post the link which one do you use

I'm on topical rn but i'm going to swirch over to the injectable

 

[Hexmask]

this stuff gives you cancer

Nigga just sayin shit. Good gatekeep

 

[NZb6Air]

Nigga just sayin shit. Good gatekeep

that dude is completely retarded, it oozes through all his posts

 

[Hexmask]

And ghkcu totally kills your IGF1 which is unfortunate

Tales. GHK-cu signals lowering of IGF-1 specifically in inflammatory responses in the body primarily of the expression in lung tissue to prevent bleomycin-induced pulmonary fibrosis. Stupid nigger. There's ZERO studies on any sort of systemic lowering of IGF-1. I've ran this peptide and encountered ZERO issues.

 

[NZb6Air]

Tales. GHK-cu signals lowering of IGF-1 specifically in inflammatory responses in the body primarily of the expression in lung tissue to prevent bleomycin-induced pulmonary fibrosis. Stupid nigger. There's ZERO studies on any sort of systemic lowering of IGF-1. I've ran this peptide and encountered ZERO issues.

it does

 

[Hexmask]

it does

View attachment 3279753

There’s zero studies claiming as to what degree though so when you dig in further you see what I was talking about. I even used Ai to go through studies. Absolutely nothing. If I had to guess it’s to no noticeable degree. But when in regards to inflammation that’s beneficial. Inflammation influences cancer.

 

[Deleted member 71543]

GHK-Cu, on paper, seems to be the single best looksmaxxing compound there is (perfect skin, anti-aging, surgery recovery, neurogenesis⁹,¹¹ etc)¹, yet no one seems to be reaping any of its benefits. What’s even more bizarre is that injection-related pain is massively reported, more consistently so than its supposed benefits².

Quickly, Glycyl-L-histidyl-L-lysine (GHK) is a tripeptide isolated from human plasma³, which possesses a high affinity for copper (II) ions, with which it spontaneously forms a complex (GHK-Cu). GHK alone has been shown to be responsible for the collagen boost⁴ and no injection-related pain has been reported with it⁵, so go for it if you want but it's the added copper that is responsible for this myriad of blessings;

EXAMPLES :
The complex, unlike GHK alone, helps activate matrix metalloproteinases, which break down damaged tissue so it can be replaced with healthy tissue⁶.

View attachment 3275853

OR

Compositions of copper (II) containing compounds have been show to accelerate the healing of wounds in warm-blooded animals, and more specifically the systemic loading of copper (II) compounds to accelerate the rate of wound healing following injury or surgery to warm-blooded animals.⁷

View attachment 3275859


; ...and consequently what seems to cause tissue damage during injection.


If we look at the cayman chemicals reviews of the molecules in question :


View attachment 3275820View attachment 3275821


The main differences are the addition of a copper atom and a significantly worse solubility profile, which suggests a causal relationship between these two differences (meaning that the added copper induces the worse solubility).

In fact, we know that metals are gay

Spoiler: Gay

Gay meaning they have different bonding properties vs carbon, oxygen, etc (this whole field of gay metals is “inorganic chemistry”) The reason for this is that metals basically have up to ten relevant electrons , which have comically complex geometries, are much larger (radius) and less bound to the nucelus

View attachment 3275870

Simply, they like to move a lot, they're playful building blocks that like to change and mix , while other atoms are stronger and stick together better, other atoms, like those in water or air, are like super strong blocks that hold together really well. They don’t like to change as much, so they are usually more stable.




Seriously, metals require ions to stabilize in various contexts, primarily due to their tendency to lose electrons and form positive charges, the ions in PBS can neutralize the positive charge of the copper ions, reducing the likelihood of oxidation or other reactions that would destabilize the copper. PBS also maintains a stable pH, which is important for keeping copper in its soluble form. In bac water or regular saline, changes in pH can lead to precipitation or oxidation of copper ions. (5 to 7 for bac and reg saline vs 7.2 to 7.4 for Phosphate Buffered Saline)

Hence why GHK-Cu needs PBS (again because the ionic solution (has a lot of ions) stabilizes the added copper), otherwise the copper-nitrogen bonds are likely to break.
View attachment 3276079


The common folk seems to be dissolving GHK-Cu in Bac Water since that is the usual commercially available and promoted solvent for peptides².
However, Bac Water has a lower ion concentration than PBS (which makes the copper less likely to be stable), I hypothesize that the pain comes from the free copper atoms raping your tissues (not fully understood).

View attachment 3275864

Injection solutions :

Phosphate Buffered Saline :

https://sci-hub.ru/10.1172/JCI116842

View attachment 3275998

https://onlinelibrary.wiley.com/doi/epdf/10.1002/jor.22831

View attachment 3276003

https://sci-hub.ru/10.1046/j.1523-1747.1999.00606.x

View attachment 3276026


i.e. what is mostly used in studies and what is recommended by CaymanChem

Making PBS at home looks annoying af so just fucking buy it : https://www.sigmaaldrich.com/US/en/product/sigma/d8537

WORKS AND STABLE



Normale saline (0.9% saline) :

https://d1wqtxts1xzle7.cloudfront.net/37163377/Massey_Patt_Aoust_The_Effect_of_GHK-Cu_on_the_Healing_of_Diabet_Ulcers_A_Pilot_Sutyd_WOUNDS_4_21-28_1992-libre.PDF?1427753974=&response-content-disposition=attachment%3B+filename%3DMassey_Patt_Aoust_The_Effect_of_GHK_Cu_o.pdf&Expires=1730648621&Signature=bEi6x2QXzs~nLvVNqdEnYmv7~ruaYJ3wGxGnHPUBnbcUmupFy9OMJu7w2M8FOsNd6ksfpzr4TNj-7184G5MT1rk224QmlJ37S6yYnjzsLTWhqjIZqtcp~Lsf6Qy~xxDEnhMbmh~DjIE891q1xoUH4l6V73w5kDgB3C-e~V06E1gnd9vJi0sdDLj5Eo0gGSVBWxV3fF2IF7cfHo9l36YXHJgRoFpOyyYKZjlF3rN-dWJ6C7VRRq8iAz8YgtCExeNyzDJA0l0HYIjE5xS65171U10rPPBRcZ5aOFzhieuFk7awwfSbKg5COwUvLmUzf9RyiTSZKci8BhSDDSbQYj6h-A__&Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA

Direct on-site injection tho

View attachment 3276031


View attachment 3276018View attachment 3276019

WORKS BUT THEORETICALLY NOT STABLE, since the PH will be too low

You'd need to adjsut it yourself⁷

View attachment 3276064

BAC WATER :

DOESN'T WORK (YOU ONLY GET GHK AND INJECTION SITE DAMAGE)²




Topical :

Topical formulations of GHKcu have been developed and are effective

BetTar:

The best one (both low-cost, most effective and most stable) being Bet Tar which is a recent (2023) chinese developped ionic-liquid-based delivery vehicle composed of betaine and tartaric acid

View attachment 3276085

View attachment 3276086
View attachment 3276090

Stable as fuck :
View attachment 3276087

PDI is dispersity

I will be attaching the papers⁸ in case President Xi takes them down

Hyaluronic acid-based hydrogel embedded with GHK peptide nanofibers : ( )

Works af for direct wound healing

In situ photo-crosslinkable hyaluronic acid-based hydrogel embedded with GHK peptide nanofibers for bioactive wound healing

A versatile hydrogel was developed for enhancing bioactive wound healing by introducing the amphiphilic GHK peptide (GHK-C16) into a photo-crosslinkab…

www.sciencedirect.com

View attachment 3276113

YES BRO I WILL TOTALLY MAKE THIS jfl

View attachment 3276114


THERE ARE 8 BILLION GOOD TOPICAL ways but WE CAN NOT MAKE/BUY any of these

This was good for wound closure but go figure how they made it

https://sci-hub.ru/10.1046/j.1524-475X.1994.20406.x

View attachment 3276058View attachment 3276117

Concering DMSO :

IT DOESN'T WORK (IT'S EVEN LESS STABLE THAN BAC WATER since it has less ions (water (bac water) has H+ and OH- (water)))

These niggas tried to study its structure while dissolving it in DMSO¹⁰ : https://sci-hub.ru/10.1016/0162-0134(89)84011-5, which gave them completly retarded results "under their conditions"

Metals in general seem to be struggling in DMSO
View attachment 3276159

Goodluck rabbitholemaxxing this

https://sci-hub.ru/10.1016/j.ccr.2004.02.005

TLDR :



- RECONSTITUTE GHK-Cu IN PBS or DPBS (which you can buy) and inject it daily

- BUY and RECONSTITUTE GHK IN BAC WATER IF YOU ONLY WANT COLLAGEN BOOST AND EASE OF USE and inject it daily

- TRY TO MAKE a [Bet] [Tar] GHK-Cu Topical Liquid Solution, if you want, for when you can't inject or as first-aid

Spoiler: Tags

@Hexmask @N1666 @Clavicular @NorwoodAscender @meathead

¹Pickart, L., & Margolina, A. (2018). Regenerative and protective actions of the GHK-Cu peptide in the light of the new gene data. International Journal of Molecular Sciences, 19(7), https://pmc.ncbi.nlm.nih.gov/articles/PMC6073405/pdf/ijms-19-01987.pdf
²
³Pickart L, Thaler MM. Tripeptide in human serum which prolongs survival of normal liver cells and stimulates growth in neoplastic liver. Nat New Biol. 1973 May 16;243(124):85-7. PMID: 4349963.
⁴Maquart, F.-X., Pickart, L., Laurent, M., Gillery, P., Monboisse, J.-C., & Borel, J.-P. (1988). Stimulation of collagen synthesis in fibroblast cultures by the tripeptide-copper complex glycyl-L-histidyl-L-lysine-Cu²⁺. Biochemical and Biophysical Research Communications, 238(2), 343-346. https://sci-hub.ru/10.1016/0014-5793(88)80509-X
⁵ Google it and add Reddit and various forums to the key-words
⁶Siméon, A., Emonard, H., Hornebeck, W., & Maquart, F.-X. (2000). The tripeptide-copper complex glycyl-L-histidyl-L-lysine-Cu²⁺ stimulates matrix metalloproteinase-2 expression by fibroblast cultures. Life Sciences, 67(20), 2257-2265. https://sci-hub.ru/10.1016/S0024-3205(00)00803-1
⁷ Pickart, L. R. (1992). Method of using copper(II) containing compounds to accelerate wound healing (U.S. Patent No. 5164367). U.S. Patent and Trademark Office. https://patentimages.storage.googleapis.com/28/c1/c7/6fb3bbcf61becb/US5164367.pdf
⁸Liu, T., Hu, L., Lu, B., Bo, Y., Liao, Y., Zhan, J., Pei, Y., Sun, H., Wang, Z., Guo, C., & Zhang, J. (2023). A novel delivery vehicle for copper peptides. New Journal of Chemistry https://pubs.rsc.org/en/content/articlelanding/2023/nj/d2nj04282g#fn1
⁹L. Pickart, in BioIogy of Copper Complexes, J. R. C. Sorenson, Ed., Human Press, Clifton, NJ, 1987, pp. 273-285 (https://library.lol/main/04A4B0236383B58B30E1AF569542355F), neurogenesis also mentionned here¹⁰
¹⁰Antholine, W. E., Petering, D. H., & Pickart, L. (1989). ESR studies of the interaction of copper(II) GHK, histidine, and Ehrlich cells. Journal of Inorganic Biochemistry, 35(2), U-224
¹¹Pickart, L., Vasquez-Soltero, J. M., & Margolina, A. (2012). The human tripeptide GHK-Cu in prevention of oxidative stress and degenerative conditions of aging: Implications for cognitive health. Oxidative Medicine and Cellular Longevity, 2012, Article ID 324832. https://doi.org/10.1155/2012/324832

Holy what a book

 

[NZb6Air]

zero studies claiming as to what degree though

We know exactly how it nukes it

 

[Hexmask]

View attachment 3279776View attachment 3279777

We know exactly how it nukes it

I never noticed the decrease. And I know what it feels like to nuke my IGF-1 cause of enclomiphene. Plus it increases life span. I think part of the reason the drug is considered an anti-aging drug is because of the effects of IGF-1 in inflammation.

 

[RAGNARAIKA]

amazing thread, bump

 

[Deleted member 71543]

what are the negative effects of ghk-cu lowering igf-1 in fully grown manlets like myself?

 

[Deleted member 71543]

Koper peptide Ghk-Cu

Bestel Koper Peptide GHK-Cu van hoge kwaliteit. Geschikt voor laboratoriumonderzoek. Snelle levering en scherpe prijs bij Laboratoriumdiscounter.

www.laboratoriumdiscounter.nl

is this any good or naur

CAS: 49557-75-7
Formula : C14H22N6O4Cu·C4H4O2
g/mol : 485.986

 

[NZb6Air]

Koper peptide Ghk-Cu

Bestel Koper Peptide GHK-Cu van hoge kwaliteit. Geschikt voor laboratoriumonderzoek. Snelle levering en scherpe prijs bij Laboratoriumdiscounter.

www.laboratoriumdiscounter.nl

is this any good or naur

CAS: 49557-75-7
Formula : C14H22N6O4Cu·C4H4O2
g/mol : 485.986

hell nawl

 

[Deleted member 71543]

hell nawl

Do you have a source for good shizzle ?

 

[aryan33]

Copper Peptide XL Edition

Entdecke die Kraft von COPPER PEPTIDE, die revolutionärste Formulierung mit der höchsten Konzentration an reinen Kupferpeptiden auf dem Markt. Sie garantiert maximale Wirksamkeit und bietet unvergleichliche Vorteile für eine straffe, glatte, strahlende und jünger aussehende Haut.

nichebeautylab.com

 

[HighClassMaxing]

4sd g

 

[Sangrit]

https://hvyresearch.com/product/bacteriostatic-water-with-pbs/
Pretty sure the risk of infection would be pretty high with a typical distilled water PBS solution. Wouldn't be able to keep it for more than a couple days once opened (without re-sterilizing). IDK though. + mayb the bac water would fucc the benefits of PBS.

 

[DORIAN]

Phosphate Buffered Saline :

Anyone tried PBS from Tracy?

I think they've had quality issues with BAC, and I'm not sure if that will carry over to PBS, too.

@NZb6Air
@halloweed
@Dyorotic.

Phosphate-buffered saline 15ml, pH= 6,96 // 10.

How do I make PBS?

 

[NZb6Air]

View attachment 3322380

Anyone tried PBS from Tracy?

I think they've had quality issues with BAC, and I'm not sure if that will carry over to PBS, too.

@NZb6Air
@halloweed
@Dyorotic.

Phosphate-buffered saline 15ml, pH= 6,96 // 10.

Yea I'm aware, in transit

 

[Deleted member 4633]

View attachment 3322380

Anyone tried PBS from Tracy?

I think they've had quality issues with BAC, and I'm not sure if that will carry over to PBS, too.

@NZb6Air
@halloweed
@Dyorotic.

Phosphate-buffered saline 15ml, pH= 6,96 // 10.

yeah we've all switched to PBS. Painless even when barely diluted

 

[ducksoover]

GHK-Cu, on paper, seems to be the single best looksmaxxing compound there is (perfect skin, anti-aging, surgery recovery, neurogenesis⁹,¹¹ etc)¹, yet no one seems to be reaping any of its benefits. What’s even more bizarre is that injection-related pain is massively reported, more consistently so than its supposed benefits².

Quickly, Glycyl-L-histidyl-L-lysine (GHK) is a tripeptide isolated from human plasma³, which possesses a high affinity for copper (II) ions, with which it spontaneously forms a complex (GHK-Cu). GHK alone has been shown to be responsible for the collagen boost⁴ and no injection-related pain has been reported with it⁵, so go for it if you want but it's the added copper that is responsible for this myriad of blessings;

EXAMPLES :
The complex, unlike GHK alone, helps activate matrix metalloproteinases, which break down damaged tissue so it can be replaced with healthy tissue⁶.

View attachment 3275853

OR

Compositions of copper (II) containing compounds have been show to accelerate the healing of wounds in warm-blooded animals, and more specifically the systemic loading of copper (II) compounds to accelerate the rate of wound healing following injury or surgery to warm-blooded animals.⁷

View attachment 3275859


; ...and consequently what seems to cause tissue damage during injection.


If we look at the cayman chemicals reviews of the molecules in question :


View attachment 3275820View attachment 3275821


The main differences are the addition of a copper atom and a significantly worse solubility profile, which suggests a causal relationship between these two differences (meaning that the added copper induces the worse solubility).

In fact, we know that metals are gay

Spoiler: Gay

Gay meaning they have different bonding properties vs carbon, oxygen, etc (this whole field of gay metals is “inorganic chemistry”) The reason for this is that metals basically have up to ten relevant electrons , which have comically complex geometries, are much larger (radius) and less bound to the nucelus

View attachment 3275870

Simply, they like to move a lot, they're playful building blocks that like to change and mix , while other atoms are stronger and stick together better, other atoms, like those in water or air, are like super strong blocks that hold together really well. They don’t like to change as much, so they are usually more stable.




Seriously, metals require ions to stabilize in various contexts, primarily due to their tendency to lose electrons and form positive charges, the ions in PBS can neutralize the positive charge of the copper ions, reducing the likelihood of oxidation or other reactions that would destabilize the copper. PBS also maintains a stable pH, which is important for keeping copper in its soluble form. In bac water or regular saline, changes in pH can lead to precipitation or oxidation of copper ions. (5 to 7 for bac and reg saline vs 7.2 to 7.4 for Phosphate Buffered Saline)

Hence why GHK-Cu needs PBS (again because the ionic solution (has a lot of ions) stabilizes the added copper), otherwise the copper-nitrogen bonds are likely to break.
View attachment 3276079


The common folk seems to be dissolving GHK-Cu in Bac Water since that is the usual commercially available and promoted solvent for peptides².
However, Bac Water has a lower ion concentration than PBS (which makes the copper less likely to be stable), I hypothesize that the pain comes from the free copper atoms raping your tissues (not fully understood).

View attachment 3275864

Injection solutions :

Phosphate Buffered Saline :

https://sci-hub.ru/10.1172/JCI116842

View attachment 3275998

https://onlinelibrary.wiley.com/doi/epdf/10.1002/jor.22831

View attachment 3276003

https://sci-hub.ru/10.1046/j.1523-1747.1999.00606.x

View attachment 3276026


i.e. what is mostly used in studies and what is recommended by CaymanChem

Making PBS at home looks annoying af so just fucking buy it : https://www.sigmaaldrich.com/US/en/product/sigma/d8537

WORKS AND STABLE



Normale saline (0.9% saline) :

https://d1wqtxts1xzle7.cloudfront.net/37163377/Massey_Patt_Aoust_The_Effect_of_GHK-Cu_on_the_Healing_of_Diabet_Ulcers_A_Pilot_Sutyd_WOUNDS_4_21-28_1992-libre.PDF?1427753974=&response-content-disposition=attachment%3B+filename%3DMassey_Patt_Aoust_The_Effect_of_GHK_Cu_o.pdf&Expires=1730648621&Signature=bEi6x2QXzs~nLvVNqdEnYmv7~ruaYJ3wGxGnHPUBnbcUmupFy9OMJu7w2M8FOsNd6ksfpzr4TNj-7184G5MT1rk224QmlJ37S6yYnjzsLTWhqjIZqtcp~Lsf6Qy~xxDEnhMbmh~DjIE891q1xoUH4l6V73w5kDgB3C-e~V06E1gnd9vJi0sdDLj5Eo0gGSVBWxV3fF2IF7cfHo9l36YXHJgRoFpOyyYKZjlF3rN-dWJ6C7VRRq8iAz8YgtCExeNyzDJA0l0HYIjE5xS65171U10rPPBRcZ5aOFzhieuFk7awwfSbKg5COwUvLmUzf9RyiTSZKci8BhSDDSbQYj6h-A__&Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA

Direct on-site injection tho

View attachment 3276031


View attachment 3276018View attachment 3276019

WORKS BUT THEORETICALLY NOT STABLE, since the PH will be too low

You'd need to adjsut it yourself⁷

View attachment 3276064

BAC WATER :

DOESN'T WORK (YOU ONLY GET GHK AND INJECTION SITE DAMAGE)²




Topical :

Topical formulations of GHKcu have been developed and are effective

BetTar:

The best one (both low-cost, most effective and most stable) being Bet Tar which is a recent (2023) chinese developped ionic-liquid-based delivery vehicle composed of betaine and tartaric acid

View attachment 3276085

View attachment 3276086
View attachment 3276090

Stable as fuck :
View attachment 3276087

PDI is dispersity

I will be attaching the papers⁸ in case President Xi takes them down

Hyaluronic acid-based hydrogel embedded with GHK peptide nanofibers : ( )

Works af for direct wound healing

In situ photo-crosslinkable hyaluronic acid-based hydrogel embedded with GHK peptide nanofibers for bioactive wound healing

A versatile hydrogel was developed for enhancing bioactive wound healing by introducing the amphiphilic GHK peptide (GHK-C16) into a photo-crosslinkab…

www.sciencedirect.com

View attachment 3276113

YES BRO I WILL TOTALLY MAKE THIS jfl

View attachment 3276114


THERE ARE 8 BILLION GOOD TOPICAL ways but WE CAN NOT MAKE/BUY any of these

This was good for wound closure but go figure how they made it

https://sci-hub.ru/10.1046/j.1524-475X.1994.20406.x

View attachment 3276058View attachment 3276117

Concering DMSO :

IT DOESN'T WORK (IT'S EVEN LESS STABLE THAN BAC WATER since it has less ions (water (bac water) has H+ and OH- (water)))

These niggas tried to study its structure while dissolving it in DMSO¹⁰ : https://sci-hub.ru/10.1016/0162-0134(89)84011-5, which gave them completly retarded results "under their conditions"

Metals in general seem to be struggling in DMSO
View attachment 3276159

Goodluck rabbitholemaxxing this

https://sci-hub.ru/10.1016/j.ccr.2004.02.005

TLDR :



- RECONSTITUTE GHK-Cu IN PBS or DPBS (which you can buy) and inject it daily

- BUY and RECONSTITUTE GHK IN BAC WATER IF YOU ONLY WANT COLLAGEN BOOST AND EASE OF USE and inject it daily

- TRY TO MAKE a [Bet] [Tar] GHK-Cu Topical Liquid Solution, if you want, for when you can't inject or as first-aid

Spoiler: Tags

@Hexmask @N1666 @Clavicular @NorwoodAscender @meathead

¹Pickart, L., & Margolina, A. (2018). Regenerative and protective actions of the GHK-Cu peptide in the light of the new gene data. International Journal of Molecular Sciences, 19(7), https://pmc.ncbi.nlm.nih.gov/articles/PMC6073405/pdf/ijms-19-01987.pdf
²
³Pickart L, Thaler MM. Tripeptide in human serum which prolongs survival of normal liver cells and stimulates growth in neoplastic liver. Nat New Biol. 1973 May 16;243(124):85-7. PMID: 4349963.
⁴Maquart, F.-X., Pickart, L., Laurent, M., Gillery, P., Monboisse, J.-C., & Borel, J.-P. (1988). Stimulation of collagen synthesis in fibroblast cultures by the tripeptide-copper complex glycyl-L-histidyl-L-lysine-Cu²⁺. Biochemical and Biophysical Research Communications, 238(2), 343-346. https://sci-hub.ru/10.1016/0014-5793(88)80509-X
⁵ Google it and add Reddit and various forums to the key-words
⁶Siméon, A., Emonard, H., Hornebeck, W., & Maquart, F.-X. (2000). The tripeptide-copper complex glycyl-L-histidyl-L-lysine-Cu²⁺ stimulates matrix metalloproteinase-2 expression by fibroblast cultures. Life Sciences, 67(20), 2257-2265. https://sci-hub.ru/10.1016/S0024-3205(00)00803-1
⁷ Pickart, L. R. (1992). Method of using copper(II) containing compounds to accelerate wound healing (U.S. Patent No. 5164367). U.S. Patent and Trademark Office. https://patentimages.storage.googleapis.com/28/c1/c7/6fb3bbcf61becb/US5164367.pdf
⁸Liu, T., Hu, L., Lu, B., Bo, Y., Liao, Y., Zhan, J., Pei, Y., Sun, H., Wang, Z., Guo, C., & Zhang, J. (2023). A novel delivery vehicle for copper peptides. New Journal of Chemistry https://pubs.rsc.org/en/content/articlelanding/2023/nj/d2nj04282g#fn1
⁹L. Pickart, in BioIogy of Copper Complexes, J. R. C. Sorenson, Ed., Human Press, Clifton, NJ, 1987, pp. 273-285 (https://library.lol/main/04A4B0236383B58B30E1AF569542355F), neurogenesis also mentionned here¹⁰
¹⁰Antholine, W. E., Petering, D. H., & Pickart, L. (1989). ESR studies of the interaction of copper(II) GHK, histidine, and Ehrlich cells. Journal of Inorganic Biochemistry, 35(2), U-224
¹¹Pickart, L., Vasquez-Soltero, J. M., & Margolina, A. (2012). The human tripeptide GHK-Cu in prevention of oxidative stress and degenerative conditions of aging: Implications for cognitive health. Oxidative Medicine and Cellular Longevity, 2012, Article ID 324832. https://doi.org/10.1155/2012/324832

so all those products online claiming to be selling ghk cu peptides are just full of shit?

 

[Pop23]

How the fuck I find the topical one in Germany?

 

[muragoat]

View attachment 3322380

Anyone tried PBS from Tracy?

I think they've had quality issues with BAC, and I'm not sure if that will carry over to PBS, too.

update?

 

[true_volcel]

Do you use just the PBS that you linked, or do you mix it with bateriostatic water at some ratio to form a perfect solution. I bought some GHK-Cu from QSC, but they are probably just using bac water, unless someone knows better. I wan the full benefits of GHK-Cu

 

[Deleted member 16636]

Do you use just the PBS that you linked, or do you mix it with bateriostatic water at some ratio to form a perfect solution. I bought some GHK-Cu from QSC, but they are probably just using bac water, unless someone knows better. I wan the full benefits of GHK-Cu

it comes lyophilized(as a powder), you just buy the pbs separate and reconstitute with it

 

[muragoat]

it comes lyophilized(as a powder), you just buy the pbs separate and reconstitute with it

where to buy PBS

 

[Deleted member 16636]

where to buy PBS

he gave source in the post

 

[true_volcel]

it comes lyophilized(as a powder), you just buy the pbs separate and reconstitute with it

He has a source that already has reconstituted PBS. I was just wondering if i can just use that and reconstitute GHK-Cu power, or if i need to mix the PBS water with Bac water and then mix that with GHK-Cu powder.

 

[Deleted member 16636]

He has a source that already has reconstituted PBS. I was just wondering if i can just use that and reconstitute GHK-Cu power, or if i need to mix the PBS water with Bac water and then mix that with GHK-Cu powder.

It’s simple. Buy the ghk cu and the pbs and only reconstitute with the pbs. Don’t mix it with bac

 

[muragoat]

It’s simple. Buy the ghk cu and the pbs and only reconstitute with the pbs. Don’t mix it with bac

25 € shipment to EU, any other option?

 

[true_volcel]

It’s simple. Buy the ghk cu and the pbs and only reconstitute with the pbs. Don’t mix it with bac

alright mate. Should I just keep pinning my QSC GHK-Cu and wait until the powder comes in. This thread is making it seem like its a waste of time to do that

 

[Deleted member 16636]

alright mate. Should I just keep pinning my QSC GHK-Cu and wait until the powder comes in. This thread is making it seem like its a waste of time to do that

I assume you reconstituted that with bac water, which is still fine as you'll get the collagen boost from it. I just kept pinning mine until I received more and then I used pbs.

 

[Deleted member 16636]

25 € shipment to EU, any other option?

just suck it up and pay the price, it was the cheapest one I found anyway.

 

[true_volcel]

I assume you reconstituted that with bac water, which is still fine as you'll get the collagen boost from it. I just kept pinning mine until I received more and then I used pbs.

I bought the premade vials from QSC, so I'm assuming they're using bac water rather than PBS.

 

[true_volcel]

25 € shipment to EU, any other option?

Even if you only bought 10 grams of it, that'd be enough for 2000 doses

 

[muragoat]

I bought the premade vials from QSC, so I'm assuming they're using bac water rather than PBS.

were ghk cu already diluited?

Even if you only bought 10 grams of it, that'd be enough for 2000 doses

doesn it expire?

 

[Deleted member 16636]

I bought the premade vials from QSC, so I'm assuming they're using bac water rather than PBS.

Never heard of them making premade vials, no source will ever ship out reconstituted vials and instead keep it as a freeze dried powder (which has been the same for my orders). They do this because peptides are fragile, you don't want them to get shaken up during transportation and mess with the potency. Qsc sell bac and pbs too, so it would make more sense for them to not send out "premade" vials so people buy what they need to reconstitute with from them.

 

[true_volcel]

were ghk cu already diluited?

Yes, QSC pre-diluted it. They probably have it in just plain Bac water, and now PBW water. But they also sell powder. I'll make a order for their powder soon.

 

[true_volcel]

Never heard of them making premade vials, no source will ever ship out reconstituted vials and instead keep it as a freeze dried powder (which has been the same for my orders). They do this because peptides are fragile, you don't want them to get shaken up during transportation and mess with the potency. Qsc sell bac and pbs too, so it would make more sense for them to not send out "premade" vials so people buy what they need to reconstitute with from them.

I'm in USA. Look at their most updated listings. In the USA warehouse, they have 10x75mg vials.

 

[muragoat]

Yes, QSC pre-diluted it. They probably have it in just plain Bac water, and now PBW water. But they also sell powder. I'll make a order for their powder soon.

seems strange? so it lost its effectiveness?

 

[true_volcel]

seems strange? so it lost its effectiveness?

i have no idea mate. According to the original post, it did lose its potency if its in bac water. It loses its ions or something like that. The PBS water keeps the copper atoms together.

 

[muragoat]

Yes, QSC pre-diluted it. They probably have it in just plain Bac water, and now PBW water. But they also sell powder. I'll make a order for their powder soon.

@20/04/2008 @Giorgio

 

[nuttheb]

GHK-Cu, on paper, seems to be the single best looksmaxxing compound there is (perfect skin, anti-aging, surgery recovery, neurogenesis⁹,¹¹ etc)¹, yet no one seems to be reaping any of its benefits. What’s even more bizarre is that injection-related pain is massively reported, more consistently so than its supposed benefits².

Quickly, Glycyl-L-histidyl-L-lysine (GHK) is a tripeptide isolated from human plasma³, which possesses a high affinity for copper (II) ions, with which it spontaneously forms a complex (GHK-Cu). GHK alone has been shown to be responsible for the collagen boost⁴ and no injection-related pain has been reported with it⁵, so go for it if you want but it's the added copper that is responsible for this myriad of blessings;

EXAMPLES :
The complex, unlike GHK alone, helps activate matrix metalloproteinases, which break down damaged tissue so it can be replaced with healthy tissue⁶.

View attachment 3275853

OR

Compositions of copper (II) containing compounds have been show to accelerate the healing of wounds in warm-blooded animals, and more specifically the systemic loading of copper (II) compounds to accelerate the rate of wound healing following injury or surgery to warm-blooded animals.⁷

View attachment 3275859


; ...and consequently what seems to cause tissue damage during injection.


If we look at the cayman chemicals reviews of the molecules in question :


View attachment 3275820View attachment 3275821


The main differences are the addition of a copper atom and a significantly worse solubility profile, which suggests a causal relationship between these two differences (meaning that the added copper induces the worse solubility).

In fact, we know that metals are gay

Spoiler: Gay

Gay meaning they have different bonding properties vs carbon, oxygen, etc (this whole field of gay metals is “inorganic chemistry”) The reason for this is that metals basically have up to ten relevant electrons , which have comically complex geometries, are much larger (radius) and less bound to the nucelus

View attachment 3275870

Simply, they like to move a lot, they're playful building blocks that like to change and mix , while other atoms are stronger and stick together better, other atoms, like those in water or air, are like super strong blocks that hold together really well. They don’t like to change as much, so they are usually more stable.




Seriously, metals require ions to stabilize in various contexts, primarily due to their tendency to lose electrons and form positive charges, the ions in PBS can neutralize the positive charge of the copper ions, reducing the likelihood of oxidation or other reactions that would destabilize the copper. PBS also maintains a stable pH, which is important for keeping copper in its soluble form. In bac water or regular saline, changes in pH can lead to precipitation or oxidation of copper ions. (5 to 7 for bac and reg saline vs 7.2 to 7.4 for Phosphate Buffered Saline)

Hence why GHK-Cu needs PBS (again because the ionic solution (has a lot of ions) stabilizes the added copper), otherwise the copper-nitrogen bonds are likely to break.
View attachment 3276079


The common folk seems to be dissolving GHK-Cu in Bac Water since that is the usual commercially available and promoted solvent for peptides².
However, Bac Water has a lower ion concentration than PBS (which makes the copper less likely to be stable), I hypothesize that the pain comes from the free copper atoms raping your tissues (not fully understood).

View attachment 3275864

Injection solutions :

Phosphate Buffered Saline :

https://sci-hub.ru/10.1172/JCI116842

View attachment 3275998

https://onlinelibrary.wiley.com/doi/epdf/10.1002/jor.22831

View attachment 3276003

https://sci-hub.ru/10.1046/j.1523-1747.1999.00606.x

View attachment 3276026


i.e. what is mostly used in studies and what is recommended by CaymanChem

Making PBS at home looks annoying af so just fucking buy it : https://www.sigmaaldrich.com/US/en/product/sigma/d8537

WORKS AND STABLE



Normale saline (0.9% saline) :

https://d1wqtxts1xzle7.cloudfront.net/37163377/Massey_Patt_Aoust_The_Effect_of_GHK-Cu_on_the_Healing_of_Diabet_Ulcers_A_Pilot_Sutyd_WOUNDS_4_21-28_1992-libre.PDF?1427753974=&response-content-disposition=attachment%3B+filename%3DMassey_Patt_Aoust_The_Effect_of_GHK_Cu_o.pdf&Expires=1730648621&Signature=bEi6x2QXzs~nLvVNqdEnYmv7~ruaYJ3wGxGnHPUBnbcUmupFy9OMJu7w2M8FOsNd6ksfpzr4TNj-7184G5MT1rk224QmlJ37S6yYnjzsLTWhqjIZqtcp~Lsf6Qy~xxDEnhMbmh~DjIE891q1xoUH4l6V73w5kDgB3C-e~V06E1gnd9vJi0sdDLj5Eo0gGSVBWxV3fF2IF7cfHo9l36YXHJgRoFpOyyYKZjlF3rN-dWJ6C7VRRq8iAz8YgtCExeNyzDJA0l0HYIjE5xS65171U10rPPBRcZ5aOFzhieuFk7awwfSbKg5COwUvLmUzf9RyiTSZKci8BhSDDSbQYj6h-A__&Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA

Direct on-site injection tho

View attachment 3276031


View attachment 3276018View attachment 3276019

WORKS BUT THEORETICALLY NOT STABLE, since the PH will be too low

You'd need to adjsut it yourself⁷

View attachment 3276064

BAC WATER :

DOESN'T WORK (YOU ONLY GET GHK AND INJECTION SITE DAMAGE)²




Topical :

Topical formulations of GHKcu have been developed and are effective

BetTar:

The best one (both low-cost, most effective and most stable) being Bet Tar which is a recent (2023) chinese developped ionic-liquid-based delivery vehicle composed of betaine and tartaric acid

View attachment 3276085

View attachment 3276086
View attachment 3276090

Stable as fuck :
View attachment 3276087

PDI is dispersity

I will be attaching the papers⁸ in case President Xi takes them down

Hyaluronic acid-based hydrogel embedded with GHK peptide nanofibers : ( )

Works af for direct wound healing

In situ photo-crosslinkable hyaluronic acid-based hydrogel embedded with GHK peptide nanofibers for bioactive wound healing

A versatile hydrogel was developed for enhancing bioactive wound healing by introducing the amphiphilic GHK peptide (GHK-C16) into a photo-crosslinkab…

www.sciencedirect.com

View attachment 3276113

YES BRO I WILL TOTALLY MAKE THIS jfl

View attachment 3276114


THERE ARE 8 BILLION GOOD TOPICAL ways but WE CAN NOT MAKE/BUY any of these

This was good for wound closure but go figure how they made it

https://sci-hub.ru/10.1046/j.1524-475X.1994.20406.x

View attachment 3276058View attachment 3276117

Concering DMSO :

IT DOESN'T WORK (IT'S EVEN LESS STABLE THAN BAC WATER since it has less ions (water (bac water) has H+ and OH- (water)))

These niggas tried to study its structure while dissolving it in DMSO¹⁰ : https://sci-hub.ru/10.1016/0162-0134(89)84011-5, which gave them completly retarded results "under their conditions"

Metals in general seem to be struggling in DMSO
View attachment 3276159

Goodluck rabbitholemaxxing this

https://sci-hub.ru/10.1016/j.ccr.2004.02.005

TLDR :



- RECONSTITUTE GHK-Cu IN PBS or DPBS (which you can buy) and inject it daily

- BUY and RECONSTITUTE GHK IN BAC WATER IF YOU ONLY WANT COLLAGEN BOOST AND EASE OF USE and inject it daily

- TRY TO MAKE a [Bet] [Tar] GHK-Cu Topical Liquid Solution, if you want, for when you can't inject or as first-aid

Spoiler: Tags

@Hexmask @N1666 @Clavicular @NorwoodAscender @meathead

¹Pickart, L., & Margolina, A. (2018). Regenerative and protective actions of the GHK-Cu peptide in the light of the new gene data. International Journal of Molecular Sciences, 19(7), https://pmc.ncbi.nlm.nih.gov/articles/PMC6073405/pdf/ijms-19-01987.pdf
²
³Pickart L, Thaler MM. Tripeptide in human serum which prolongs survival of normal liver cells and stimulates growth in neoplastic liver. Nat New Biol. 1973 May 16;243(124):85-7. PMID: 4349963.
⁴Maquart, F.-X., Pickart, L., Laurent, M., Gillery, P., Monboisse, J.-C., & Borel, J.-P. (1988). Stimulation of collagen synthesis in fibroblast cultures by the tripeptide-copper complex glycyl-L-histidyl-L-lysine-Cu²⁺. Biochemical and Biophysical Research Communications, 238(2), 343-346. https://sci-hub.ru/10.1016/0014-5793(88)80509-X
⁵ Google it and add Reddit and various forums to the key-words
⁶Siméon, A., Emonard, H., Hornebeck, W., & Maquart, F.-X. (2000). The tripeptide-copper complex glycyl-L-histidyl-L-lysine-Cu²⁺ stimulates matrix metalloproteinase-2 expression by fibroblast cultures. Life Sciences, 67(20), 2257-2265. https://sci-hub.ru/10.1016/S0024-3205(00)00803-1
⁷ Pickart, L. R. (1992). Method of using copper(II) containing compounds to accelerate wound healing (U.S. Patent No. 5164367). U.S. Patent and Trademark Office. https://patentimages.storage.googleapis.com/28/c1/c7/6fb3bbcf61becb/US5164367.pdf
⁸Liu, T., Hu, L., Lu, B., Bo, Y., Liao, Y., Zhan, J., Pei, Y., Sun, H., Wang, Z., Guo, C., & Zhang, J. (2023). A novel delivery vehicle for copper peptides. New Journal of Chemistry https://pubs.rsc.org/en/content/articlelanding/2023/nj/d2nj04282g#fn1
⁹L. Pickart, in BioIogy of Copper Complexes, J. R. C. Sorenson, Ed., Human Press, Clifton, NJ, 1987, pp. 273-285 (https://library.lol/main/04A4B0236383B58B30E1AF569542355F), neurogenesis also mentionned here¹⁰
¹⁰Antholine, W. E., Petering, D. H., & Pickart, L. (1989). ESR studies of the interaction of copper(II) GHK, histidine, and Ehrlich cells. Journal of Inorganic Biochemistry, 35(2), U-224
¹¹Pickart, L., Vasquez-Soltero, J. M., & Margolina, A. (2012). The human tripeptide GHK-Cu in prevention of oxidative stress and degenerative conditions of aging: Implications for cognitive health. Oxidative Medicine and Cellular Longevity, 2012, Article ID 324832. https://doi.org/10.1155/2012/324832

thank you for the subway surfers i almost stoped reading

 

[Deleted member 19325]

yall niggas dumb if you can’t understand this.

thank fuck ghk-cu injectable will never be widely used. improved my skin quality within days of using and got comments on it having improved so ik it’s not placebo. the other benefits are nice too

How much do u inject daily?

 

[tyronmaxxing]

Cool and awesome thread. Now just where to find Betaine-Tartaric Acid (BetTar) based GHK-Cu solution.

 

[Pop23]

Gonna add tiny amount to my minoxidil to reap the benefits faster.